Development of a disposable sensor for electrocatalytic detection of guanine and ss-DNA using a modified sol-gel screen-printed carbon electrode

The electrocatalytic oxidation of guanine and DNA is demonstrated on a sol-gel coated carbon screen printed electrode modified with {MeReO(edt)}₂ using cyclic and differential pulse voltammetric techniques. An oxidation peak at 370 mV was found, but no corresponding reduction peaks could be detected in the negative scan, which indicates that the oxidation of guanine is completely irreversible process. The oxidation peak potentials are shifted to more negative values with increasing pH. The utility of applying the sensor for determination of guanine and ss-DNA were investigated. The linear ranges were 0.19-10.8 and 0.45-7.8 μg ml⁻¹ for guanine and DNA, respectively. Detection limits of 0.1 and 0.32 μg ml⁻¹ were obtained for guanine and ss-DNA, respectively.     

Multiobjective shape optimization of speed humps

This paper presents a new approach to the shape optimization of road speed humps. The proposed approach is based on multiobjective genetic optimization of the hump profile while taking into account the separation phenomenon, which occurs when the front tires of the vehicle momentarily lose contact with the road surface. The optimization is carried out for speeds up to twice the authorized speed (throughout this article, the term authorized speed refers to speed limits enforced in speed reduction [bump] areas of the roads) rather than for illegally high speeds as adopted by many of the previous works. A 6-degree of freedom non-linear dynamic model is used to identify the speeds at which separation occurs, and hump profiles associated with these speeds are discarded as infeasible solutions. Three independent objective functions are selected for optimization. They include the maximum vertical acceleration experienced by the driver when crossing the hump below the authorized speed limit (to be minimized), the same vertical acceleration at speeds above the authorized speed (to be maximized), and the ascending ratio of the “speed—vertical acceleration” curve (to be maximized). These objective functions are evaluated for more than 10,000 humps of two popular profile types (sinusoidal and flat top with straight ramps) and optimum profiles for three speed limits of 20, 25, and 30km/h are determined using the multiobjective nondominated sorting genetic algorithm II. As a result, a Pareto front of at least ten optimal points is achieved for each of the two hump profile types. Furthermore, to incorporate the economical aspects of the real-world problem, Pareto optimal points for the two profile types were compared based on their lateral section areas (an indication of the manufacturing cost). The comparison shows that sinusoidal humps more often than not outdo their flat top rivals economically.     

A microprocessor-based adaptive power factor corrector fornonlinear loads

A microprocessor-based adaptive power factor corrector for poor power factor (linear or nonlinear) loads is introduced. The system power factor is measured by the microprocessor and compared with a predetermined reference value. Accordingly, the microprocessor adjusts the power factor to get the predetermined value. This is achieved by controlling the firing angle of a thyristorized static VAR (volt-ampere reactive) compensator through microcomputer software. The system power factor is measured by the microprocessor at every supply cycle, and the above sequence is repeated. The proposed scheme achieves both accurate measurement and adjustment of the system power factor     

Effects of amniotic fluid embolism-like plasma on isolated perfused rabbit lungs

Information on road injury with analyses on the epidemiological features and determinants of fatality rate with prevention and control of road injury was presented in this paper. From 1951 to 1994, road injury and motor vehicle deaths had a continuous increase by 43 and 78 times respectively. Since 1987 about 250,000 cases, 5000 deaths and 15,000 trauma cases caused by road crashes year after year. The fatality rate increased by an average of 12.9% increase per year from 1984 to 1994 (from 2.43 to 5.56 per 100,000 pop). Zhejiang, Guangdong, Liaoning, Sichuan and Jiangsu occupied the first five places in 30 provinces, accounted for over 45% of total road injuries and one thirds of road fatalities. Both incidence and death rate were lowest in Beijing City and highest in Zhejiang Province. Three-quarters of the fatality on road injury were occurred on smooth and straight pavement in sunny days. Over 60% of the motor vehicle deaths were young males (age 20-50 yrs). Classified by occupation, majority of the victims were peasants (55%) and workers (19%). Two thirds of the road fatalities were pedestrians, bicycle riders and passengers. Human factors including driver's irresponsibility, non-licensing, bicycle rider and pedestrian carelessness, etc. were notified that accounted for over 90% of the causes for road injury. Primary causes from the drivers were rules and regulations violation, absent-mindedness, driving after drinking alcohol. The risk factors of road injuries were the increase of motor vehicles and traffic congestion. It is also necessary to improve the traffic safety knowledge among residents.     

Putative mechanism for anticancer properties of Ag–PP (NPs) extract

One of the most important challenges in treating cancer is the invasion and the angiogenesis of cancer cells. The synthesis of green nanoparticles (NPs) and their use in therapeutic fields is one of the most effective methods with minimal side effects in cancer treatment. In this study, cytotoxic and anti‐angiogenic effects of silver NPs (AgNPs) coated with palm pollen extract [Ag–PP(NPs)] were evaluated. For this purpose, the cells were treated with NPs and then were subjected to trypan blue testing (48 h). Then, the cancer invasion was evaluated by the scratch procedure and the expressions of the vascular endothelial growth factor (VEGF) and its receptor (VEGF‐R) genes were estimated using real‐time PCR assay. Also, the angiogenesis effect of the NPs was investigated with chick chorioallantoic membrane (CAM) assay. The Ag–PP(NPs) induced cytotoxicity on MCF7 cells. The findings also showed that Ag–PP(NPs) inhibit invasive cancer cells and reduce the expression of VEGF and VEGF‐R and significantly reduced the number and vessels lengths and the lengths and weights of the embryos in CAM assay. Ag–PP(NPs) with the induction of cytotoxic effects, metastatic inhibition and anti‐angiogenesis properties should be considered as an appropriate option for treatment of cancerInspec keywords: nanomedicine, genetics, cellular biophysics, toxicology, patient treatment, silver, cancer, biochemistry, biomedical materials, nanoparticles, nanofabrication, membranesOther keywords: minimal side effects, cancer treatment, silver NPs, cancer invasion, vascular endothelial growth factor, receptor genes, VEGF‐R, real‐time polymerase chain reaction assay, angiogenesis effect, chick chorioallantoic membrane assay, MCF7 cells, invasive cancer cells, cytotoxic effects, putative mechanism, anticancer properties, antiangiogenic effects, antiangiogenesis properties, Ag–PP‐induced cytotoxicity, metastatic inhibition, palm pollen extraction, trypan blue testing, time 48.0 hour, Ag     

Physical and mechanical properties of the fully interconnected chitosan ice‐templated scaffolds

Porous chitosan scaffolds were prepared with a freeze‐casting technique with different concentrations, 1.5 and 3 wt %, and also different cooling rates, 1 and 4°C/min. The pore morphology, porosity, pore size, mechanical properties, and water absorption characteristics of the scaffolds were studied. Scanning electron microscopy images showed that the freeze‐cast scaffolds were fully interconnected because of the existence of pores on the chitosan walls in addition to many unidirectionally elongated pores. Increases in the chitosan concentration and freezing rate led to elevations in the thickness of the chitosan walls and reductions in the pores size, respectively. These two results led to the enhancement of the compressive strength from 34 to 110 kPa for the scaffolds that had 96–98% porosity. Also, augmentation of the chitosan concentration and decreases in the freezing rate led to the reduction of the number of pores on the chitosan walls. Furthermore, the volume of water absorption increased with a reduction in the chitosan concentration and cooling rate from 690 to 1020%. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41476.     

Optimal placement of steel plate shear walls for steel frames by bat algorithm

Layout optimization of steel frames with steel plate walls (SPWs) using a meta‐heuristic search algorithm is the main aim of the present study. SPWs are lateral load‐resisting systems, especially against earthquake excitation. These systems offer significant advantages in terms of cost, performance and ease of design compared with other systems. In this study, orthotropic membrane model is used to model the behaviour of steel plate shear walls. The newly developed bat algorithm, which is based on the echolocation behaviour of bats, is employed as the present study optimizer. Design variables of the optimization problem consist of the cross sections of beams and columns of the frame, the web plate thicknesses of SPWs and the placement of SPW in the frame. The bat algorithm performs suitable selection of sections from the AISC wide‐flange (W) shapes list. Strength constraints of the American Institute of Steel Construction Load and Resistance Factor Design and displacement constraints are checked during the optimization process. The results reveal the effectiveness of the proposed method for optimization of steel frames with SPWs. Copyright © 2014 John Wiley & Sons, Ltd.     

A survey of decorative materials on the energy consumption of mid-rise residential buildings in Mashad, Iran

Polyvinylchloride (PVC) panel is one of the most favorite decorative materials that has been popularly applied as finishing of ceiling in residential buildings. It is about five years that the people incline to redecorate the ceiling of old buildings with PVC panel in big cities of Iran, such as Mashad. In this study, the influence of ceiling PVC panel on the cooling and heating loads of studied apartment were determined by software DeST-h. In addition, the summer natural ventilation of the mentioned apartment is investigated by determining the wind speed into the apartment through the computational fluid dynamics (CFD) software. The evaluation of environment indoor wind velocity showed that most of the apartment space is a comfortable zone. The results of studied building analyses demonstrated that using PVC panel on the ceiling can decline the energy consumption of the penthouse (fifth level) of the investigated building, which is about 3.7% and 7% for studied methods of without and with air layer, respectively. In addition, although the existence of air layer can decline the cooling and heating loads, the increase in air layer thickness did not show significant decrease on building energy consumption. However, the PVC panel is expensive and is not suitable to be used for ceiling thermal insulation, but adding a thin layer of air between ceiling and PVC panel can be a good step toward sustainable building, when the people are inclined to utilize it as a decorative ceiling.     

An innovative approach to electro-oxidation of dopamine on titanium dioxide nanotubes electrode modified by gold particles

Au/TiO₂/Ti electrodes were prepared by galvanic deposition of gold particles from an acidic bath containing KAu(CN)₂ in the presence of a citrate buffer onto TiO₂ nanotubes layer on titanium substrates. Titanium oxide nanotubes were fabricated by anodizing titanium foil in a DMSO fluoride-containing electrolyte. The morphology and surface characteristics of Au/TiO₂/Ti electrodes were investigated using scanning electron microscopy and energy-dispersive X-ray, respectively. The results indicated that gold particles were homogeneously deposited on the surface of TiO₂ nanotubes. The nanotubular TiO₂ layers consist of individual tubes of about 40–80 nm diameters. The electro-catalytic behavior of Au/TiO₂/Ti electrodes for the dopamine electro-oxidation was studied by cyclic voltammetry and differential pulse voltammetry. The results showed that Au/TiO₂/Ti electrodes exhibit a considerably higher electro-catalytic activity toward the oxidation of dopamine. The catalytic oxidation peak current showed a linear dependence on dopamine concentration and a linear calibration curve was obtained in the concentration range of 0.5–2.5 mM of dopamine.