嗜水气单胞菌RecA和Gap-2作为内参蛋白的评价

为确定嗜水气单胞菌中可用于Western Blot的适宜内参蛋白,考察了重组酶RecA和3-磷酸甘油醛脱氢酶Gap-2在该菌不同培养阶段及培养条件下的表达稳定性.以嗜水气单胞菌基因组为模板扩增recA和gap-2基因,并对蛋白进行异源表达.利用亲和层析和凝胶过滤层析技术纯化蛋白,制备多克隆抗体.通过Western Blot技术研究了RecA和Gap-2蛋白在嗜水气单胞菌不同培养时间、不同培养温度和培养环境中铁离子浓度差异条件下的表达情况.结果显示,重组表达载体pET-28a-recA和pET-28a-gap-2构建成功,可与His标签融合表达.纯化后得到了纯度较高的RecA和Gap-2,以二者为抗原制备的多克隆抗体效价均达到1:512000.Western Blot结果表明,RecA在细菌生长稳定期表达情况与前期有所差异,而Gap-2蛋白在该菌不同培养时期、温度及铁离子浓度差异的环境中表达较为恒定.研究表明,Gap-2更适合作为嗜水气单胞菌Western Blot检测用内参蛋白.     

一类随机蚊虫种群模型及其渐近性态

基于沃尔巴克氏菌影响下的确定性蚊虫种群模型,考虑受感染的蚊虫出生率受到环境中随机噪声的影响,建立了一类新的随机蚊虫种群模型,并研究了该随机模型的适定性和长时间渐近性态.首先,通过伊藤公式并选用适当的李雅普诺夫函数,证明了全局正解的存在唯一性;其次,定义此模型的基本再生数R0,证明了当R0<1时,受感染的蚊虫数将趋于灭绝;接着,给出模型随机平均持久的充分条件;最后,通过一个数值例子说明了定理的应用.     

云制造环境下新能源汽车供应链协同制造模式

为了解决新能源汽车制造企业生产能力资源非均衡低匹配的问题,提出云制造环境下新能源汽车供应链协同制造模式,构建了这一模式下的体系架构,并交代了云制造环境下新能源汽车供应链协同制造模式的运作流程,且分析了这一制造模式下时间、成本和价格等方面的优势,也更进一步表明这一制造模式实现的必要性,为新能源汽车的生产制造提供了一种新的途径.     

2＋2×0.32ST超高强度钢丝帘线在半钢子午线轮胎带束层中的应用

研究2+2×0.32ST钢丝帘线替代2+2×0.35HT钢丝帘线在半钢子午线轮胎带束层中的应用。结果表明:与2+2×0.35HT钢丝帘线相比,2+2×0.32ST钢丝帘线的强度更高、直径更小、耐疲劳性能更好;在轮胎带束层中用2+2×0.32ST钢丝帘线替代2+2×0.35HT钢丝帘线后,工艺性能良好,成品轮胎的充气外缘尺寸变化不大,强度性能、高速性能、耐久性能和耐水压性能均能满足设计和国家标准要求,滚动阻力降低,同时有效减小了轮胎中的钢丝帘线和胶料用量,降低了生产成本。     

粗粒料三轴试验橡皮膜嵌入量数值模拟

在粗粒料三轴试验中,橡皮膜嵌入量可造成显著的体积变形量测误差。本文针对颗粒材料的接触特点,利用Nagata Patch方法重建颗粒表面,基于重建曲面进行接触状态的判断和接触几何信息的计算,开发了高效的颗粒接触算法。该法采用dual mortar有限元方法处理颗粒与橡皮膜间的接触模拟,针对橡皮膜变形较大的特点,采用更新坐标的大变形计算格式,并根据重建的颗粒表面对颗粒-橡皮膜的距离进行几何修正,实现了颗粒-橡皮膜接触的精细化模拟,可较好地模拟计算“柔性”橡皮膜嵌入颗粒孔隙的过程。进行了Kramer钢球试验以及粗颗粒料和标准粗砂三轴试验橡皮膜嵌入过程的模拟计算,计算结果符合一般规律,与相应的试验结果吻合较好,验证了本文方法对于粗粒料膜嵌入问题的适用性。     

Technology for recycling and regenerating graphite from spent lithium-ion batteries

With the annual increase in the amount of lithium-ion batteries (LIBs), the development of spent LIBs recycling technology has gradually attracted attention. Graphite is one of the most critical materials for LIBs, which is listed as a key energy source by many developed countries. However, it was neglected in spent LIBs recycling, leading to pollution of the environment and waste of resources. In this paper, the latest research progress for recycling of graphite from spent LIBs was summarized. Especially, the processes of pretreatment, graphite enrichment and purification, and materials regeneration for graphite recovery are introduced in details. Finally, the problems and opportunities of graphite recycling are raised.     

关于高压电气设备带电检测信息化管理的研究

随着电力系统的发展,对发电、输电、供电和用电的可靠性要求越来越高,高压电气设备状态检测也尤显重要.建立了一种智慧仪器仪表在线检测智能运检平台,制定统一的数据传输规范,实现检测数据终端分析、检测结果线上管理,全面提升电力设备带电检测试验工作水平,支撑泛在电力物联网重要战略部署.     

基于5G基站节能技术的智慧型断路器研究与设计

研究分析了5 G基站节能站点增强型节能的技术方案.主要采用AAU设备关断的方法,实现设备关闭期间断路器保持分闸状态,减少AAU能耗的效果.设计开发了一套基于物联网技术的智能型微型断路器,包括断路器和网关模块,实现设备端时段分合闸控制.最后通过实际案例数据,论证该方法在实际基站节能降耗的效果.     

Preparation of High Strength and Toughness Aramid Fiber by Introducing Flexible Asymmetric Monomer to Construct Misplaced-Nunchaku Structure

High performance fibers with high strength and toughness have great potential in composites, but contradiction between tensile strength and elongation at break makes the preparation to become a current challenge. Herein, an asymmetric structure of more flexible diamine, 3,4′-diaminodiphenyl ether (3,4′-ODA), is introduced into heterocyclic aramid (PBIA) fibers to replace rigid symmetric p-phenylenediamine (PDA). By studying the properties of copolymer (mPEBA) fibers with different ratios of diamine, it is found that the mPEBA fiber reached the optimal mechanical properties with the 30% content of 3,4′-ODA. Compared with homopolymerized heterocyclic aramid fibers, the tensile strength and elongation at break of mPEBA fiber are improved by 26.2% and 38.7%, respectively. Results of X-ray diffraction show that the introduction of 3,4′-ODA structure can increase stretchability of mPEBA fibers, improving the orientation degree during hot-drawing. Molecular dynamics simulations confirm that 3,4′-ODA structure undergoes a conformation transformation to form a straightened chain during hot-drawing, while symmetrical 4,4′-diaminodiphenyl ether (4,4′-ODA) cannot form the same conformation. The misplaced-nunchaku structure is formed based on the special meta-para position of 3,4′-ODA, achieving the synergy of high strength and high toughness.     

A Dual Physical Cross-Linking Strategy to Construct Tough Hydrogels with High Strength,Excellent Fatigue Resistance,and Stretching-Induced Strengthening Effect

Hydrogels with excellent stiffness, toughness, anti-fatigue, and self-recovery properties are regarded as promising water-containing materials. In this work, a dual physically cross-linked (DPC) sodium alginate (SA)/poly[acrylamide (AAm)-acrylic acid (AAc)-octadecyl methacrylate (OMA)]-Fe³⁺ hydrogel is reported, which is constructed by hydrophobic association (HA) and ionic coordination (IC). The optimal DPC hydrogel demonstrates excellent mechanical performance: tensile modulus of 0.65 MPa, tensile strength of 3.31 MPa, elongation at break of 1547%, and toughness of 27.8 MJ m^–3. SA/P(AAm-AAc-OMA)-Fe³⁺ DPC hydrogels also exhibit prominent anti-fatigue and self-recovery performance (99.1–109.7% modulus recovery and 90.4–108.9% dissipated energy recovery after resting for 5 min without additional stimuli at ambient temperature) through the reconstruction of reversible physical cross-linking. Some of the SA/P(AAm-AAc-OMA)-Fe³⁺ DPC hydrogels even exhibit a stretching-induced strengthening effect, which is similar to the performance of muscle—“the more training, the more strength.” Hence, the combination of HA and IC will provide an effective approach to design DPC hydrogels with desirable mechanical performances and a longer service life for wider applications of soft materials.