Research on the impact of service provider cooperative relationship on cloud manufacturing platform

As an emerging service-oriented manufacturing paradigm, Cloud Manufacturing (CMfg) is expected to provide smart networked platforms that enable efficient global collaborations among cloud service providers in a product lifecycle. The service provider cooperative relationship (SPCR) plays a crucial part in cloud service scheduling and thus potentially influences the operational performance of a CMfg platform. The guaranteed SPCR could ensure service quality and reduce the interaction costs among service providers in the process of collaborative manufacturing tasks, thereby having a positive impact on the operational efficiency of a CMfg platform. The cost reduction and quality assurance, however, usually make the cost-effective services more attractive for an increasing number of manufacturing tasks and lead to load imbalance in a CMfg platform, thus having a negative impact on the operational efficiency. The impact mechanism of SPCR on a CMfg platform is still unclear from an evolutionary perspective. In this paper, we propose a CMfg platform evolution model (CPEM) to investigate the impacts of SPCR on the operational performance of a CMfg platform. Three quantitative metrics, from the perspective of cloud consumer, cloud operator, and cloud service provider, respectively, are established to support the performance evaluation. The results of contrast experiments reveal the impacts of SPCR on a CMfg platform.     

Accessible regions of tool orientations in multi-axis milling of blisks with a ball-end mill

Noninterference tool orientations are hard to be determined in multi-axis milling of blisks, because the integrated structure of blisks introduces more geometrical constraints. To address this problem, an original approach without interference detection is proposed to solve accessible regions of tool orientations in milling of blisks. Based on the visibility of checking surfaces, only critical points on surface profiles are searched and processed to construct the accessible regions. In this approach, the start point of each profile is sought on boundaries of the main surface with a constant step length. From this point, a new critical point is first searched along a specified direction and then adjusted iteratively until it locates on a profile. When all critical points on profiles are searched like this, the searched points on each checking surface are reordered to form one or more than one closed curve to present the accessible region related to this checking surface. These curves are then mapped onto a unit sphere and divided into nonintersecting segments. After that, a concise approach is proposed to combine accessible regions of all checking surfaces into simply connected regions. This algorithm is finally verified with two kinds of blisks and compared with a referenced method. The results show that the proposed method can efficiently solve accessible regions in multi-axis milling of blisks with an expected accuracy.     

Regulation Mechanism of Novel Thermomechanical Treatment on Microstructure and Properties in Al-Zn-Mg-Cu Alloy

Scanning electron microscopy, transmission electron microscopy, tensile test, exfoliation corrosion test, and slow strain rate tensile test were applied to investigate the properties and microstructure of Al-Zn-Mg-Cu alloy processed by final thermomechanical treatment, retrogression reaging, and novel thermomechanical treatment (a combination of retrogression reaging with cold or warm rolling). The results indicate that in comparison with conventional heat treatment, the novel thermomechanical treatment reduces the stress corrosion susceptibility. A good combination of mechanical properties, stress corrosion resistance, and exfoliation corrosion resistance can be obtained by combining retrogression reaging with warm rolling. The mechanism of the novel thermomechanical treatment is the synergistic effect of composite microstructure such as grain morphology, dislocation substructures, as well as the morphology and distribution of primary phases and precipitations.     

冗余捷联惯组故障诊断的奇异值分解新方法*

冗余捷联惯组故障诊断奇异值分解法由于缺少单位化、阈值偏大和隔离策略有缺陷,导致漏警率偏高、误隔离率偏高且无法隔离负向故障。在分析Shim法原理的基础上,采用改进的解耦矩阵、新的故障隔离函数包含单位化和取模的运算,并且将故障检测和隔离分步实施。对改进方法进行蒙特卡洛数字仿真,仿真试验结果表明:新方法克服了Shim法的缺点,具有更高的正检率、更低的误隔离率。尤其对于发生负向故障的器件,改进方法能得到约99%的隔离率。新方法为冗余捷联惯组故障诊断提供一种新思路。     

Porous polyurethane foam for use as a particle collection substrate in a nanoparticle respiratory deposition sampler

Porous polyurethane foam was evaluated to replace the eight nylon meshes used as a substrate to collect nanoparticles in the Nanoparticle Respiratory Deposition (NRD) sampler. Cylindrical (25 mm diameter by 40 mm deep) foam with 100 pores per inch was housed in a 25-mm-diameter conductive polypropylene cassette cowl compatible with the NRD sampler. Pristine foam and nylon meshes were evaluated for metals content via elemental analysis. The size-selective collection efficiency of the foam was evaluated using salt (NaCl) and metal fume aerosols in independent tests. Collection efficiencies were compared to the nanoparticulate matter (NPM) criterion and a semi-empirical model for foam. Changes in collection efficiency and pressure drop of the foam and nylon meshes were measured after loading with metal fume particles as measures of substrate performance. Substantially less titanium was found in the foam (0.173 µg sampler^?1) compared to the nylon mesh (125 µg sampler^?1), improving the detection capabilities of the NRD sampler for titanium dioxide particles. The foam collection efficiency was similar to that of the nylon meshes and the NPM criterion (R² = 0.98, for NaCl), although the semi-empirical model underestimated the experimental efficiency (R² = 0.38). The pressure drop across the foam was 8% that of the nylon meshes when pristine and changed minimally with metal fume loading (～19 mg). In contrast, the pores of the nylon meshes clogged after loading with ～1 mg metal fume. These results indicate that foam is a suitable substrate to collect metal (except for cadmium) nanoparticles in the NRD sampler.

Copyright © 2016 American Association for Aerosol Research     

Alisertib Induces Cell Cycle Arrest,Apoptosis, Autophagy and Suppresses EMT in HT29 and Caco-2 Cells

Colorectal cancer (CRC) is one of the most common malignancies worldwide with substantial mortality and morbidity. Alisertib (ALS) is a selective Aurora kinase A (AURKA) inhibitor with unclear effect and molecular interactome on CRC. This study aimed to evaluate the molecular interactome and anticancer effect of ALS and explore the underlying mechanisms in HT29 and Caco-2 cells. ALS markedly arrested cells in G₂/M phase in both cell lines, accompanied by remarkable alterations in the expression level of key cell cycle regulators. ALS induced apoptosis in HT29 and Caco-2 cells through mitochondrial and death receptor pathways. ALS also induced autophagy in HT29 and Caco-2 cells, with the suppression of phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR), but activation of 5′ AMP-activated protein kinase (AMPK) signaling pathways. There was a differential modulating effect of ALS on p38 MAPK signaling pathway in both cell lines. Moreover, induction or inhibition of autophagy modulated basal and ALS-induced apoptosis in both cell lines. ALS potently suppressed epithelial to mesenchymal transition (EMT) in HT29 and Caco-2 cells. Collectively, it suggests that induction of cell cycle arrest, promotion of apoptosis and autophagy, and suppression of EMT involving mitochondrial, death receptor, PI3K/Akt/mTOR, p38 MAPK, and AMPK signaling pathways contribute to the cancer cell killing effect of ALS on CRC cells.     

Genipin cross-linked chitosan hydrogel for the controlled release of tetracycline with controlled release property,lower cytotoxicity,and long-term bioactivity

To realize the controlled release property, lower cytotoxicity, and long-term bioactivity of tetracycline, the chitosan hydrogel had been prepared using genipin as the cross-linker and the tetracycline was in situ encapsulated. The formation process and strength of hydrogel were studied by rheological analysis, and the microtopography was observed by scanning electron microscope. It was found that the amount of genipin could modulate the physical property of the hydrogel. For in vitro release and antibacterial assays, the hydrogel could controllably release tetracycline and keep its bioactivity for a long time. The tetracycline-loaded chitosan hydrogel showed good antibacterial effect even under alkaline environment, which displayed better stability compared with free tetracycline. Moreover, the tetracycline-loaded chitosan hydrogel exhibited lower cytotoxicity than did tetracycline alone, suggesting that this tetracycline-loaded chitosan hydrogel could be a more useful dosage form than separate doses of tetracycline. The novel aspects of this study include the cytotoxicity study and the in vitro and in vivo assays, which might be useful for other researchers in this field.

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Graphical abstract To realize the controlled release property, lower cytotoxicity, and long-term bioactivity of tetracycline, genipin cross-linked chitosan hydrogel was used as the carrier of tetracycline. The hydrogel could controllably release tetracycline with bioactivity. The hydrogel showed good antibacterial effect even under alkaline environment. The hydrogel exhibited lower cytotoxicity than did tetracycline alone.

     

考虑外滚道缺陷的滚动轴承刚度时变特性分析

滚动轴承的主要失效形式之一是在滚道或滚动体表面产生缺陷,形成疲劳点蚀,对轴承性能产生很大影响。针对深沟球轴承,建立外滚道存在方形缺陷时的动力学模型,获取缺陷对载荷分布的影响规律,建立轴承时变刚度模型,分析缺陷尺寸演化和位置对轴承刚度时变特性的影响规律。结果表明:当缺陷位于非承载区时对轴承平均刚度无影响;当缺陷位于承载区时,轴承的平均刚度与缺陷长度和深度负相关;缺陷位置由底端向非承载区变化时,轴承的平均刚度先减小后增大。     

温度对WC-WB-CoCr涂层高温摩擦磨损性能的影响

采用超音速火焰喷涂(HVOF)制备了WC-WB-CoCr涂层,研究了温度对WC-WB-CoCr涂层高温摩擦磨损性能的影响。通过SEM、XRD和显微硬度仪对涂层的微观组织、相结构和力学性能进行表征。通过摩擦磨损试验机和拉曼光谱仪研究了WC-WB-CoCr涂层的高温摩擦学性能和氧化产物,采用台阶仪扫描磨痕形貌并计算WC-WB-CoCr涂层的磨损率。结果表明：WC-WB-Co-Cr涂层主要由WC和CoW₂B₂组成,涂层结构致密,与基体结合紧密;随着磨损试验温度升高,涂层的摩擦系数从0.66降低到0.57,涂层的磨损率随着温度的升高而升高,但是其磨损率增长程度随着温度的升高而降低。在高温磨损过程中,磨痕表面的氧化膜主要由WO₃和CoWO₄组成,且CoWO₄比WO₃表现出更好的耐高温磨损性能。涂层的主要磨损机制为氧化磨损、疲劳磨损和粘着磨损。     

熔盐电解法制备单一Sm2Fe17合金相的机理研究

选择在LiF-CaF₂-SmF₃熔盐体系中,采用熔盐电解法在1160℃下通过恒电流在Fe阴极表面获得了不同厚度(17.22～40.34μm)的Sm₂Fe₁₇合金层,同时通过循环伏安法与方波伏安法研究了Sm³⁺在W与Fe阴极上的电化学行为。结果表明,Sm³⁺无法单独沉积于W电极上,Sm³⁺在铁阴极上的还原为Sm⁰分为2步,首先在-0.33V(vs.Cr/Cr₂O₃)发生Sm³⁺还原为Sm²⁺的可溶-可溶型反应,然后当电极电势超过-0.78V(vs.Cr/Cr₂O₃)时Sm²⁺开始在阴极界面处上发生欠电势还原,在Fe电极表面生成Sm₂Fe₁₇合金,这是由于在Sm₂Fe₁₇合金中降...