Liquid–liquid extraction of a recombinant protein with reverse micelles

Recombinant cytochrome b₅ was extracted into the reversed micelle phase of an anionic surfactant (AOT) in octane and back-extracted to a final aqueous phase. The extraction of the protein was controlled by an electrostatic mechanism, since it was dependent on the global charge of the protein. This was directly demonstrated by experiments with native and mutant cytochromes obtained by site directed mutagenesis. The back-extraction of cytochrome b₅ to a fresh aqueous phase was decreased by factors that reduced the size of the water pool of the organic phase, such as high salt concentrations (1–2 mol dm^?3 NaCl) and low temperatures (4°C), probably because of an increase in a favourable interaction of this protein with the surfactant at closer distances.     

Change in lubricity of oils with detergent additives in the course of aging

Translated from Khimiya i Tekhnologiya Topliv i Masel, No. 10, pp. 13–16, October, 1992.     

Oscillations in a bounded volume of reacting gas suspensate

Sverdlovsk. Translated from Fizika Goreniya i Vzryva, Vol. 27, No. 1, pp. 82–88, January–February, 1991.     

Study of the polymerization of Mo(VI) in weakly acidic concentrated (10−2M) paramolybdate solutions using the Mo/Fe atomic ratio of the equilibrium iron molybdate precipitates

The polymerization of Mo(VI) is investigated using the Mo/Fe atomic ratio of the equilibrium precipitates of iron molybdates obtained with a small Fe(III) excess. The overall formation equation of isopolymolybdate ions is deduced which presents the state of molybdate ions as a function of pH and concentration of the Mo(VI) solution.     

FSK heterodyne transmission experiments at 560 Mbit/s and 1 Gbit/s

Optical frequency-shift-keying (FSK) signals are obtained from directly modulated distributed feedback (DFB) semiconductor lasers. Experimental studies of the direct frequency modulation (FM) characteristics of the DFB lasers show a nonuniform FM response due to the competing effects of thermal modulation of the laser active region and carrier density modulation. Equalization of the signal current to the laser is employed to produce a flat FM response from 30 kHz to 1 GHz. Optical FSK transmission and heterodyne detection experiments at 560-Mbit/s and 1-Gbit/s are conducted at a wavelength of 1497 nm. Receiver sensitivities of -39 dBm at 560 Mbit/s and -37 dBm at 1 Gbit/s are obtained. Transmission through 100 km of single-mode fiber at 1 Gbit/s is achieved with no degradation in receiver sensitivity.     

Survival of Yersinia enterocolitica and Escherichia coli in spring water

Survival of pure and mixed cultures of Yersinia enterocolitica and Escherichia coli in sterile spring water stored at 4 degrees C was evaluated. Y. enterocolitica increased two or three logs during the first 3 weeks of incubation, and at the end of 64 weeks viable cells in numbers corresponding to the initial inoculum level were still detectable. However, after 1 week of incubation, the number of E. coli in water started to decrease and in 13 weeks time no E. coli was detected. More rapid decrease of E. coli was observed in mixed cultures.     

Simulation of the capacitance-voltage characteristics of a ferroelectric material

Semiconductors - The parameters of a ferroelectric material without initial polarization are simulated. The ferroelectric material is doped with a shallow-level impurity. One of the contacts is...     

Personal spaces — ePerSpace

This paper describes the ePerSpace research project which aims to significantly increase user acceptance of networked audiovisual systems and applications at home and virtually anywhere by enabling innovative interoperable value-added personalised networked services and applications. Moreover, it aims to develop an open architecture and a framework that will significantly increase the speed of developing novel value-added personalised services by reusing well-defined system components with open APIs. The structure of the paper follows the methodology of the project. It starts with a vision of future personalised services from the users’ perspective by developing five scenarios. These scenarios are then used to extract the requirements of the personalisation framework which is then described in terms of its personalisation, service management, digital home environment management and content adaptation capabilities.