High-resolution analysis of T-cell receptor beta-chain repertoires using DNA heteroduplex tracking: generally stable, clonal CD8+ expansions in all healthy young adults

Harringtonine (HT), an anticancer drug with high chemotherapeutic efficiency to human chronic granulocytic/myelomonocytic leukemia, has been reported to rapidly induce apoptosis in HL-60 cells in a wide scope/range of dosage by investigators from our lab and others. In the present studies, by using video enhancement contrast (VEC) microscopy, we dynamically analyzed changes in intracellular calcium distribution in a single HL-60 cell over the period from the initiation of apoptosis to the obvious appearance of chromatin condensation. The results from this paper demonstrated the striking distinction of intracellular calcium distribution at different time points after treatment with HT. Before treatment in normal HL-60 cells the highest [Ca2+]i accumulation was observed in the peri-nuclear area and the lowest was observed in the nucleus; after treatment with 1 microg/ml HT for 30 min intracellular calcium diffused all over the cell compartments, while intranuclear calcium increased comparatively and significantly. The phenomenon of intranuclear calcium accumulation was further confirmed by using laser scanning confocal microscopy (LSCM). In addition, co-localization of the highest calcium region with condensed chromatin in apoptotic HL-60 cells was also observed by LSCM. Our results suggest that two sequential alterations of intracellular calcium distribution occurred in apoptotic HL-60 cells induced by HT, i.e. (a) accumulation of calcium in the nucleus and (b) regionalization in a specific nuclear region.     

An evaluation of ruminally degradable intake protein and metabolizable amino acid requirements of feedlot calves

Ruminally degradable intake protein (DIP) and metabolizable indispensable amino acid (MIAA) requirements of feedlot steers were evaluated. Dietary treatments consisted of isocaloric 80% concentrate steam-flaked corn-based diets containing either .8% urea, 1.5% fish meal (FM), 3.0% FM, 4.5% FM, or 4.5% soybean meal (SBM). Treatment effects on characteristics of ruminal and total tract digestion were evaluated using four Holstein steers (249 kg) with cannulas in the rumen and proximal duodenum. Ruminal digestibility of OM (RDOM; P < .05) and feed N (P < .01) and microbial N flow (MNF; P < .01) to the small intestine were greater with urea as the supplemental N source. The level of DIP was closely associated (R2 = .89) with MNF. Postruminal digestibility of OM was greater (P < .05) for FM than for urea-supplemented diets, compensating for lower RDOM. There were no treatment effects (P > .10) on DOM. As the level of FM was increased, MIAA increased linearly (P < .01). Intestinal MIAA were similar (P > .10) for urea- and SBM-supplemented diets. Treatment effects on 56-d growth performance were evaluated using 100 medium-framed crossbred steers (231 kg). Daily weight gain (linear effect; P < .01), DM intake (linear effect; P < .10), feed efficiency (linear effect; P < .05), and diet NE (linear effect; P < .05) increased with level of FM supplementation. Daily weight gain (P < .10) and DM intake (P < .05) were greater for urea- than for SBM-supplemented diets. Using bovine tissue as the reference protein, the biological value (based on chemical score) of the intestinal chyme protein averaged 73%; methionine was first-limiting. There was a close association (R2 = .99) between methionine supply to the small intestine and observed/expected dietary NE. The metabolizable methionine requirement (MMETR, g/d) of medium-framed feedlot steers can be reliably predicted from measures of BW and ADG (MMETR = 1.565 + .0234ADG[268 - (29.4 x .0557BW(.75)ADG(1.097))/ADG] + .0896BW(.75)). There was a very close association (R2 = .89) between DIP and MNF (MNF = 13.7DIP - .66DIP(2) + 25.9). At maximal observed synthesis, DIP accounted for 76% of the MNF. A minimum of 100 g DIP/kg of total tract digestible OM was required to maximize RDOM and MNF.     

测量高功率激光的体吸收能量计

叙述了体吸收激光能量计的特点，介绍了其标定方法，给出了其技术参数。体吸收激光地的推广应用取得了广泛的社会效益。     

碳酸盐岩油藏改善注水开发效果试验研究

双重孔隙介质的碳酸盐岩油藏在稳定注水采油后岩块系统还存在大量剩余油，而不稳定注水方法可在一定程度上提高水驱油效率。室内物理模型实验、数值模拟计算及现场试验结果一致表明：在碳酸盐岩油藏注水开发后期采用衰竭式降压开采，周期注水等不稳定注水方式，能够提高水驱油效率，改善油藏开发效果，获得良好的经济效益。     

新疆东昆仑地区金矿成矿地质条件及找矿方向初探

东昆仑地区是昆仑巨型成矿带中２个重要的成矿区之一，从大地构造背景、沉积建造、岩浆活动等成矿地质条件的分析入手，对东昆仑地区的求勉雷克成矿远景区、鸭子泉成矿远景区、库木巴彦成矿远景区和石雪尖成矿远景区的金矿成矿条件进行了初步的探讨，指出了找矿方向。     

Pre-bainitic Transformation in Fe-Ni Alloy

Ｔｈｅｐｒｅ－ｂａｉｎｉｔｉｃｐｈｅｎｏｍｅｎｏｎｉｓｏｎｅｏｆｔｈｅｉｍ－ｐｏｒｔａｎｔｓｕｂｊｅｃｔｓｏｆｂａｉｎｉｔｉｃｔｒａｎｓｆｏｒｍａｔｉｏｎｗｈｉｃｈｉｓｓｔｉｌｃｏｎｔｒｏｖｅｒｓｉａｌ．Ｏｎｅｖｉｅｗｉｓｔｈａｔｔｈｅｓｏｌｕｔｅａ...     

特种钢用结晶器铜管破裂原因分析

通过对某特钢厂破裂的结晶器铜管进行金相实验、扫描电镜和能谱分析,并结合结晶器的生产、使用及失效过程情况,综合受力分析,认为铜管破裂原因主要是环境介质硫渗入形成硫化物,在拉应力作用下,裂纹沿脆性质点扩展导致的破坏。     

羧酸酯稀土对聚氯乙烯的热老化稳定作用研究

用热烘法研究了羧酸酯稀土对聚氯乙烯的热老化稳定作用。利用紫外／可见光谱分析跟踪共轭双键在热老化过程中的变化，比较ＣＥＲＥＳ，有机锡及两者的复合稳定剂的热老化稳定性能，发现ＣＥＲＥＳ与有机锡的稳定性能相当，复合稳定剂具有协同效应。     

UniMAT PLC在塑料挤出机控制系统中的应用

针对塑料挤出机控制系统成本高、速度低、精度低的问题,提出采用UniMATPLC控制方式,实践证明效果良好。     

图像隐写算法评估体系的解释结构模型研究

隐写算法的评估是以隐写系统安全性角度出发,总结出了抗检测性、鲁棒性、抗容量探测性等多个性能指标,构建了评估指标集.并采用解释结构模型法对评估指标体系进行分析。重点讨论了方法的应用和结果的分析,给出了对图像隐写算法评估体系建立和分析的思路与方法。