Isolation of a genetic locus associated with metronidazole resistance in Helicobacter pylori

We examined the molecular mechanism of metronidazole resistance by constructing a lambda-Zap II phagemid expression library with genomic DNA from a metronidazole-resistance strain of Helicobacter pylori. Twenty-two clones were found to have elevated MTZ resistances in XLOLR strain of E. coli. Phagemids belonging to the twenty two clones were extracted and then retransformed into the XLOLR strain of E. coli. After MTZ selection, five clones could confer metronidazole resistance consistently. According to Southern hybridization and DNA sequencing, the five clones contained a same locus, recA. In addition, transforming the five clones into BL21 strain of E. coli produced a higher resistance to MTZ. Interestingly, electroporation of one of the five phagemid clones into two MTZ sensitive H. pylori yielded MTZ resistant strains. Comparing amino acid sequence in MTZ resistant with sensitive isolates revealed two point mutations at this locus. Above results suggest that mutation in recA may be associated with metronidazole resistance of H. pylori.     

Prognostic factors of early sequelae and fatal outcome of Japanese encephalitis

A clinical case control study to identify prognostic factors present at hospital admission associated with early sequelae and fatal outcome of acute Japanese encephalitis (JE) was carried out in Gusi county, Henan Province, central China from June to September 1991. A total of 70 patients with laboratory-confirmed acute JE were studied, of whom 3 cases died and 33 cases had neurological or psychiatric sequelae at the end of three months follow-up. The results showed that acute JE at younger age, with higher body temperature, high white cell count in CSF, and deep coma present at hospital admission were markers for unfavorable outcomes (sequelae or fatal). A history of the vaccination was not correlated with the early sequelae and fatal outcome of the disease. The paper suggests that early diagnosis and treatment and universal JE vaccination for all susceptible populations are keys for decreasing incidence of sequelae and fatal outcome of acute JE.     

冶金炉渣氮化物容量研究的进展

本文总结了近年冶金炉渣氮酸盐容量的研究方法，氮在炉渣中存在形态与炉渣氧分压的关系，炉渣氮化物容量与温度及炉渣成分的关系，并给出设计钢液脱氮渣系的基本原则。     

不同工作制曲柄连杆式飞剪机的设计

通过对曲柄连杆式飞剪机工作制和工作特点的分析,介绍了不同工作制飞剪机的设计要点,给出了在设计中应遵循的原则,从而使其设计更加合理.     

中国公用电话业务发展值得借鉴的几个案例

随着技术和需求的不断发展,电信市场逐步走向开放,竞争更加激烈。如何抓住信息技术革命的新契机,充分掌握公话的发展方向,成为电信运营商和设备供应商的首要课题。从不同国家的公话案例得到的重要启示是:①无论是发达国家还是发展中国家,归根结底都是市场需求决定了公话的发展趋势;②作为一个运营商,首先要考虑如何优化运营成本,避免重复建设,降低总投资,缩短项目周期,同时还要引进和利用世界上最先进的技术,突破传统语音电路交换网络的限制,利用多种接入方式开展业务;③从全球来看,通信业务将从传统的话音业务逐渐向数据业务发展,运营商可以利用数字通信的优势,为广大公众用户提供个性化数字信息服务     

油砂地层中子-伽玛能谱Monte-Carlo模拟

本工作应用自行研制开发的中子 伽玛能谱测井的中子 伽玛射线随空间、能量、时间分布的蒙特卡罗模拟软件包计算了裸眼井、井眼里无下井仪器、油饱和砂岩地层、井眼注油条件下沿井轴和井壁的中子 伽玛射线随能量、时间分布及沿井轴和沿井壁的中子 伽玛射线能谱 ,研究了源距对中子 伽玛射线随能量、时间分布以及井轴与井壁中子 伽玛射线能谱的影响。     

面向对象中文窗口开发环境：C—VISION

Ｃ－ＶＩＳＩＯＮ中文窗口系统综合采用了面向对象与窗口环境设计的程序方法，彻底更新了在微机上进行中文窗口设计的编程思想，同时作为一个基础系统，为软件设计节约了大量的人力和时间，实现了微机程序的共享。