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151.
Interfacial hybridization kinetics of oligonucleotides immobilized onto fused silica surfaces 总被引:3,自引:0,他引:3
Jiang Zeng Amer Almadidy James Watterson Ulrich J. Krull 《Sensors and actuators. B, Chemical》2003,90(1-3):68-75
Fused silica optical fibers have been used in an intrinsic mode optical configuration as biosensors for fluorescence based detection of hybridization of nucleic acids. In this work, the kinetics of hybridization of single-stranded oligonucleotides that were covalently immobilized were studied. The probe DNA was dT20, and the target was Fluorescein-labeled non-complementary (dT20) or complementary (dA20) oligonucleotide. Chronofluorimetric monitoring of the adsorption and hybridization processes was used to investigate oligonucleotide films of different density, in different salt concentrations, at temperatures of 25 and 40 °C, with the concentration of the target DNA being 0.005–0.1 μM. Mathematical models based on first- and second-order Langmuir adsorption have been examined to describe both the adsorption and the hybridization processes. Experimental data were processed using the models, and the hybridization kinetics were calculated. Hybridization kinetics on these optical fiber DNA sensors was found to be up to three orders faster than results presented for a number of other experiments using different immobilization chemistries. 相似文献
152.
蒋光祖 《上海电机学院学报》2003,6(4):9-10
推导了变压器二次负载电压的计算公式,建议由此公式代替以往原理书中惯用的近似公式。利用此公式绘制和分析了不同功率因数额定负载时的相量轨迹图。 相似文献
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LILin LIUWen-li LUBin JUGuo-xian ZHANGYong-ming HAOYong-qin SUWei ZHONGJing-chang 《半导体光子学与技术》2004,10(3):179-181,193
Spectral and structural characteristics of distributed Bragg reflector (DBR) in vertical-cavity surface-emitting lasers were studied with photoluminescence and double- crystal X- ray diffraction measurement. The expected high quality epitaxial DBR structure was verified. In the X- ray double- crystal rocking curves of DBR the zeroth- order peak, the first and second order satellite peaks were measured. Splitting of diffraction peak appeared in the rocking curves was analyzed. The effects of introduced deep energy levels on the structural perfection and optical properties were discussed. 相似文献
155.
WANGYu-tian LIUZhan-wei HOUPei-guo SHANWei 《半导体光子学与技术》2004,10(3):199-202
The distributed optical fiber sensing technology is overviewed, which is based on Raman scattering light theory. Basic operation principle, structure, system characteristics and signal processing are discussed. This structure and method of the signal processing possess of certain spatial resolution, hence will ensure the practicability of system. 相似文献
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Kubat M. Hafez A. Raghavan V.V. Lekkala J.R. Wei Kian Chen 《Knowledge and Data Engineering, IEEE Transactions on》2003,15(6):1522-1534
Association mining techniques search for groups of frequently co-occurring items in a market-basket type of data and turn these groups into business-oriented rules. Previous research has focused predominantly on how to obtain exhaustive lists of such associations. However, users often prefer a quick response to targeted queries. For instance, they may want to learn about the buying habits of customers that frequently purchase cereals and fruits. To expedite the processing of such queries, we propose an approach that converts the market-basket database into an itemset tree. Experiments indicate that the targeted queries are answered in a time that is roughly linear in the number of market baskets, N. Also, the construction of the itemset tree has O(N) space and time requirements. Some useful theoretical properties are proven. 相似文献
160.