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71.
Transglutaminase (TGase) was separated from the culture broth of an isolated strain of Streptoverticillium mobaraense. The crude enzyme was prepared by centrifugation, ultrafiltration, precipitation by alcohol, centrifugation and freeze‐drying. The yield after these processes was 65–70%. Then the enzyme was purified to homogeneity by chromatography on CM‐cellulose and Sephadex G‐75 on which the yields were about 70% and 80%, respectively; the purified folds reached 2.5–4.7 and 1.08–2.06, respectively. The molecular weight of this TGase was 39,500–40,100 Da by gel filtration chromatography. Optimum enzyme activity was observed in the pH range of 5.0–7.0, and it was maintained stable at 20–40C. The optimal temperature and pH was 52C and 6.0, respectively. At 1 mM and 5 mM metal ion or inhibitors concentration, TGase activity was strongly inhibited by Zn2+ and NEM, and not affected obviously by Ba2+, Ca2+, Co2+, Cu2+, Fe2+, Fe3+, Mg2+, Mn2+, Na+ as well as PMSF and EDTA. The effects of these additions on this TGase were compared with those of other microbial TGases. 相似文献
72.
A high-density gas jet supersonic nozzle is reported in this paper. The jitter and actuation time of the nozzle is determined by the pin discharge and laser spark radiation respectively. The jitter time of the nozzle is within 10μs with the backing pressure as high as 25 bar. With a nanosecond laser pulse focused on the gas jet about 1 mm below the nozzle, the actuation time is calculated to be about 15 ms by detecting the laser produced spark radiation, which reveals the existence of the gas jet and the relative gas density evolving with time. Consequently the gas density is estimated to be well above 10^19 cm^-3, compared with theoretical simulations from the nozzle parameters. 相似文献
73.
74.
JIAHong-bo CHENBao-xue ZHOUJian-zhong ZHAODe-xir LUHong-liang YUANYi-fang ISOMamoru 《半导体光子学与技术》2004,10(1):21-27
Based on the measurement of dispersion characteristic and birefractive index of the fluorinated polyimide film,a statistical optimum design method is proposed and used to realize the design of 32-and 36-wavelengths optical waveguide wavelength-interleave coupler (i,e.,interleaver) with the optimization of polarization fluctuation and wavelength interval of 0.8nm at 1550nm. The largest cross coupling ratios of the two interleavers are respectively less than 1.8% and 3.5%, while the least through coupling ratios are respectively greater than 98. 2% and 96. 5%. The output differences due to polarization fluctuation are less than 1.7% and 3.2% 相似文献
75.
76.
LILin LIUWen-li LUBin JUGuo-xian ZHANGYong-ming HAOYong-qin SUWei ZHONGJing-chang 《半导体光子学与技术》2004,10(3):179-181,193
Spectral and structural characteristics of distributed Bragg reflector (DBR) in vertical-cavity surface-emitting lasers were studied with photoluminescence and double- crystal X- ray diffraction measurement. The expected high quality epitaxial DBR structure was verified. In the X- ray double- crystal rocking curves of DBR the zeroth- order peak, the first and second order satellite peaks were measured. Splitting of diffraction peak appeared in the rocking curves was analyzed. The effects of introduced deep energy levels on the structural perfection and optical properties were discussed. 相似文献
77.
CHENSheng-ping LUKe-cheng LIYi-gang LIJia-fang ZHUJian-ping FENGMing LIJing 《半导体光子学与技术》2004,10(2):122-126
The effects of the variations in fiber length, fiber mirror reflectance on efficiency and output power are experimentally investigated for erbium-doped double pass backward superfluorescent fiber sources (SFSs). The influence of fiber length on mean wavelength stability (MWS) has also been demonstrated. By incorporating a short section of un-pumped erbium-doped fiber (EDF) at the output port, the pump power dependent on MWS becomes independent of pumped EDF length. This is a novel phenomenon that hasn‘t been reported up to now,and should be helpful to SFS fabrication and theory analysis. By using a fiber Michelson interferometer as spectrum slicing component,a multi-wavelength fiber source (MWFS) with ~20 channels (from 1542nm to 1559nm) is got. The MWFS has a channel spacing of ~0. 8 nm which satisfies ITUstandard. The intensity fluctuation among channels is less than 0.5dB,and the extinction ratio of all channels is above 14dB. This kind of MWFS should be useful to wavelength division multiplexing systems. 相似文献
78.
79.
采用柠檬酸-硝酸盐法合成了碱土金属双掺杂的Pr0.6CaxSr0.4-xFeO3-δ(X=0.0-0.4)系列复合氧化物粉体,用TG-DTA,XRD,FT-IR,SEM等手段对产物中钙钛矿物相的形成及微结构进行了表征,结果表明,样品在800℃焙烧2h后显现钙钛矿结构的主要衍射峰;随热处理温度的升高,XRD谱峰强度逐渐增大,1000℃煅烧2h即得到正交钙钛矿结构的单相固溶体;系列样品结晶化程度和晶粒尺寸随样品中Ca含量的增大而减小;电导率测试结果表明,Ca^2+、Sr^2+双掺杂样品的电导率随Sr^2+含量的增大而增大,低温段(T≤650℃)烧结陶瓷体的导电行为遵循小极化子导电机制。 相似文献
80.
端电压比较法直接寻找无刷直流电动机换相点及其斩波调速 总被引:4,自引:0,他引:4
提供了一种利用端电压比较结合软件算法直接寻找直流无刷无位置传感器电动机换相点的方案,解决了直流无刷无位置传感器电动机控制的关键问题-转子位置检测,并以单片机PIC16C72为核心构成先进的数字控制系统,实现了软件斩波调速。 相似文献