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991.
Bracho-Troconis C Colon P Bartout JD Bienvenu Y 《Journal of materials science. Materials in medicine》2000,11(1):1-9
The mercury content of dental amalgams is a controversial subject with regard to the biological properties of these materials. The object of this study is to optimize the thermal treatments performed on an experimental powder in order to obtain a low mercury ratio (41% by weight) while preserving the desirable clinical qualities of the material. Using atomized powder, two types of thermal treatments are performed: A1, to obtain a partially annealed structure and A2, to obtain a complete homogenization. The kinetics of the amalgamation reaction is mainly evaluated by X-ray diffraction to identify the newly formed phases as a function of setting time. Mechanical properties are evaluated according to the ISO norms at 37 °C. Metallographical examination of the amalgams shows a «Ag-Hg» phase which acts as a matrix incorporating the «Cu-Sn» and «Ag-Sn» compounds. The setting kinetics of the A1 amalgams is linear and slightly more rapid than that of the A2 amalgam. The mechanical properties of the amalgams are significantly improved regarding the higher mercury content commercial amalgams. A specific thermal treatment permits us to slow down the diffusion of mercury between the different intermetallic compounds into the powder. The final amalgam composition, thus, most approaches the stoechiometric ratio calculated from a quaternary diagram. 相似文献
992.
Moya KL Salès N Hässig R Créminon C Grassi J Di Giamberardino L 《Microscopy research and technique》2000,50(1):58-65
We examined the localization of PrP(c) in normal brain using free-floating section immunohistochemistry and monclonal antibody 3F4. In the mature hamster and baboon brain, PrP(c) is localized to the neuropil with a synaptic distribution and the PrP(c) immunoreactivity is denser in regions known for ongoing plasticity. Cell bodies and major fiber tracts have little or no PrP(c) immunoreactivity. At the electron microscopic level, PrP(c) immunoreactivity decorates synaptic profiles, both pre- and postsynaptically. Results obtained with two additional antibodies, 3B5 and Pri-304, showed similar patterns of PrP(c) bands on Western blots, although Pri-304 was less sensitive. On sections through the adult hamster hippocampus, 3B5 and Pri-304 both stained the synaptic neuropil while cell bodies in the pyramidal and dentate granule cell layers were not immunoreactive. Pri-304 differentiated between synaptic layers in the hippocampus and closely resembled the pattern of staining obtained with 3F4. Preliminary results of developing brain showed that PrP(c) is initially localized along fiber tracts in the neonate brain. These results show that PrP(c) has a synaptic distribution in the adult brain and suggest that there are important changes in its distribution during brain development. These results also characterize two additional reagents for studies of PrP(c) localization. 相似文献
993.
Why so many forms of acetylcholinesterase? 总被引:4,自引:0,他引:4
Legay C 《Microscopy research and technique》2000,49(1):56-72
Acetylcholinesterase is a key molecule in the control of cholinergic transmission. In the mammalian neuromuscular junction (NMJ), the efficiency of this phenomenon depends on the enzyme location, between the presynaptic site where acetylcholine is released and the postsynaptic membrane where the acetylcholine receptors are packed. Various molecular forms of the enzyme that possess the same catalytic activity are expressed. The relative amounts of these forms are tissue-specific. At the subcellular level, this panoply of forms allows the enzyme to be attached to the membrane or to the basal lamina. Analysis of the forms secreted and their position in the cytoarchitecture of the NMJ is essential to understand the functioning of this synapse. This review will consider the origin of the enzyme polymorphism and its physiological implication. 相似文献
994.
Ramírez EI Vázquez-Salinas C Rodas-Suárez OR Pedroche FF 《Journal of food protection》2000,63(4):542-544
A total of 160 meat product samples were collected from commercial outlets in Mexico City to investigate the presence of different species of Yersinia by the 4 degrees C enrichment method after 1, 3, 5, and 7 days of incubation using alkaline treatment and isolating in cefsulodin-Irgasan-novobiocin and MacConkey agars with Tween 80. Overall, Yersinia spp. were isolated from 27% of the samples analyzed, whereas 40% of the raw and only 13% of the precooked samples were contaminated. Although 2,970 colonies showed Yersinia characteristics, only 706 (24%) actually corresponded to this genus: 49% were Yersinia enterocolitica, 25% Yersinia kristensenii, 15% Yersinia intermedia, 9% Yersinia frederiksenii, and 2% Yersinia aldovae; 10% corresponded to biotype 2, 2% to biotype 3, and 4% to biotype 4. The presence of Yersinia in raw and cooked meat products represents a health risk for consumers in Mexico, where further clinical studies are needed to assess the epidemiological importance of this pathogen. 相似文献
995.
LC/NMR and LC/MS (the latter technique in the MSn mode) were used to characterize the organic constituents of industrial wastewater with emphasis on polar, nonvolatile compounds. In the effluent of a textile company, various compounds such as anthraquinone-type dyes and their byproducts, a fluorescent brightener, a byproduct from polyester production, and auxiliaries such as anionic and nonionic surfactants and their degradation products were identified. It is shown that the combined use of both hyphenated techniques provides complementary structural information. If applied under comparable chromatographic conditions, they are well-suited for the nontarget analysis. 相似文献
996.
A new algorithm for cloud detection over sea surface has been developed that makes use of autocorrelation and cross correlation between a real spectrum and either a synthetic or a laboratory spectrum. The scheme is intended for high-spectral-resolution satelliteborne infrared sensors that will measure the Earth's entire emission spectrum rather than the upwelling radiance in a few channels. A new index is defined with which one can determine quantitatively the degree of homogeneity of two spectra in the 800-900-cm(-1) (11.11-12.5 mum) window region. The index makes use of only the observed spectrum along with a reference synthetic spectrum and, therefore, may form the basis for an operational stand-alone cloud-detection algorithm for next-generation high-spectral-resolution infrared sensors. Application both to synthetic spectra obtained in simulation and to sea-surface real spectra recorded through the interferometric monitor for greenhouse gases is considered. 相似文献
997.
With the aim of reducing surface reflections and increasing the diffraction efficiency we investigated the superposition of subwavelength phase gratings onto blazed phase gratings. With direct-write electron-beam lithography bare blazed gratings and blazed gratings carrying subwavelength gratings were fabricated and their optical performances compared. For TE polarization the subwavelength-carrying gratings showed a maximum diffraction efficiency of 90.6%, whereas the corresponding maximum value for the bare grating was 86.3%. The experiment was simulated with rigorous diffraction theory. 相似文献
998.
Synthesis of gray-level computer-generated holograms allows for an increase of the information storage capability that is usually achieved with conventional binary filters. This is mainly because more degrees of freedom are available. We propose to profit from this feature by synthesizing complex filters formed by many superimposed holograms, each with a different carrier frequency. We apply these gray-level filters to perform multichannel correlation and in this way enhance the capability of optical correlators to process the information in parallel and simultaneously. First, we analyze the behavior of some performance criteria on the impulse response and on the correlation as a function of the number of holograms that are multiplexed. Then we show the results of two experiments: In the first a composed phase-only filter is used in a multiple-object recognition process. In the second a composed synthetic discriminant function filter is used to implement an object classification by means of a binary code. 相似文献
999.
A simple fabrication technique for nonlinear polymeric optical waveguide patterns is introduced based on the two-beam interference method. We determined that the second-order nonlinearity of poled polymer films is erased by single-pulse ultraviolet (UV) laser irradiation. The erasure mechanism for second-order nonlinearity is discussed. To form a periodic structure in an optical polymer waveguide, two types of optical configuration of two-beam interference were arranged, and a single-pulse UV laser was exposed directly onto poled films. We prove that this method provides a simple way to fabricate volume-type and ridge-type periodically poled structures, i.e., chi((2)) gratings, from the submicrometer to the millimeter range. 相似文献
1000.
We discuss the modulation in the spectrum generated by the windows of a Pockels cell. Although they are antireflection coated, the residual reflectivity of the highly parallel window faces can still have etalon effects that lead to pronounced spectrum modulation. This problem is easily solved by the use of slightly wedged windows. 相似文献