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排序方式: 共有237条查询结果,搜索用时 46 毫秒
81.
Amina Ben Abla Guilhem Boeuf Ahmed Elmarjou Cyrine Dridi Florence Poirier Sylvie Changotade Didier Lutomski Abdellatif Elmselmi 《International journal of molecular sciences》2021,22(14)
Engineering of biomimetic motives have emerged as promising approaches to improving cells’ binding properties of biomaterials for tissue engineering and regenerative medicine. In this study, a bio-adhesive ligand including cell-binding domains of human fibronectin (FN) was engineered using recombinant protein technology, a major extracellular matrix (ECM) protein that interacts with a variety of integrins cell-surface’s receptors and other ECM proteins through specific binding domains. 9th and 10th fibronectin type III repeat containing Arginine-Glycine-Aspartic acid (RGD) and Pro-His-Ser-Arg-Asn (PHSRN) synergic site (FNIII9-10) were expressed in fusion with a Colored Multi Affinity Tag (CMAT) to develop a simplified production and characterization process. A recombinant fragment was produced in the bacterial system using E. coli with high yield purified protein by double affinity chromatography. Bio-adhesive surfaces were developed by passive coating of produced fragment onto non adhesive surfaces model. The recombinant fusion protein (CMAT-FNIII9/10) demonstrated an accurate monitoring capability during expression purification and adsorption assay. Finally, biological activity of recombinant FNIII9/10 was validated by cellular adhesion assay. Binding to α5β1 integrins were successfully validated using a produced fragment as a ligand. These results are robust supports to the rational development of bioactivation strategies for biomedical and biotechnological applications. 相似文献
82.
Naouel Klibi Leila Ben Said Ahlem Jouini Karim Ben Slama Maria López Rym Ben Sallem Abdellatif Boudabous Carmen Torres 《Meat science》2013
Antimicrobial resistance and the mechanisms implicated were studied in 119 enterococci from 105 meat samples from Tunisian markets. Almost 24.5% of recovered enterococci showed resistance against four or more antimicrobial agents and these isolates were identified to the species level. Enterococcus faecalis was the most prevalent species (41%). High percentages of erythromycin and tetracycline resistances were found among our isolates, and lower percentages were identified to aminoglycosides, ciprofloxacin and chloramphenicol. All tetracycline-resistant isolates carried the tet(M) and/or tet(L) genes. The erm(B) gene was detected in 78.5% of erythromycin-resistant isolates, ant(6)-Ia gene in 58.8% of streptomycin-resistant isolates, and cat(A) gene in one chloramphenicol-resistant isolate. Forty-eight isolates carried the gelE gene and exhibited gelatinase activity. The hyl and esp genes were detected in one and three Enterococcus faecium isolates, respectively. Streptomycin-resistant isolates showed a high genetic diversity by PFGE and MLST. Meat might play a role in the spread through the food chain of enterococci with these virulence and resistance characteristics to humans. 相似文献
83.
David Afungchui Baddreddinne Kamoun Ali Helali Abdellatif Ben Djemaa 《Renewable Energy》2010,35(1):307-313
The aim of this paper is to numerically explore the non-linear two-dimensional unsteady potential flow over a Savonius rotor and to develop a code for predicting its aerodynamics performances. In the model developed, the rotor is represented in a median plane by two semicircles, displaced along their common diameter. The two semicircles can be considered to produce lifting effects. As a result, they are modelled by a collection of discrete vortices on their contours. The flow field is then governed by the Laplace equation. The versatile Neumann boundary condition, applied over the contour of the semicircles and the Kutta Joukowsky condition applied at the four extremities of the semicircles have been used in the modelling. The torque distribution of the stationary rotor and the unsteady pressure field on the blades of the rotating rotor, predicted by the code developed, have been compared and validated by some experimental data. 相似文献
84.
Giuseppe Marsala Marcello Pucci Gianpaolo Vitale Maurizio Cirrincione Abdellatif Miraoui 《Applied Energy》2009
After a brief introduction about fuel cell systems, and their modelling, this paper proposes a possible solution to emulate a proton exchange membrane fuel cell (PEM-FC) system by using a DC–DC buck converter. The fuel cell system, including all its auxiliaries and related control systems, is emulated by a buck converter realized experimentally and controlled in the DSPACE environment. The realization of the buck converter allows the behaviour of any fuel cells to be easily emulated since only the modification of the control law of the switch is necessary. The proposed emulator can be applied easily to other fuel cell systems if the polarization curve has the same current rate and maximum power. In this way it is possible to utilize the converter and perform the necessary tests to optimize a fuel cell system by avoiding the waste of hydrogen and the purchase of cells as well as any cell damage. With regard to current other types of emulators, the one presented here has the following characteristics: (1) all the auxiliaries of the system have been considered, each including its own control system, as in a real FCS, (2) the converter is a classical buck converter with a free-wheeling diode and is designed to have a high bandwidth and to be practically always in conduction mode (discontinuous mode appears only at very low currents) (3) the voltage control is made by a space-state controller, able to fix properly the closed loop poles of the system, thus guaranteeing the desired bandwidth of the control system and (4) it can be used in laboratory as a stand-alone low-cost system for design and experimental purposes. 相似文献
85.
86.
Najjari A Ouzari H Boudabous A Zagorec M 《International journal of food microbiology》2008,121(3):342-351
In Tunisia, several food products derived from meat or seafood are naturally processed, without any addition of bacterial starters. Such fermented, dried-cured, salted, or marinated products, as well as the raw meat or fish may thus provide a source to isolate the natural microflora colonizing such environments. We isolated lactic acid bacteria from a representative range of flesh-foods sold or manufactured in different parts of Tunisia, and selectively searched for Lactobacillus sakei, a lactic acid bacterium potentially useful as starter or protective culture. Eighty six (86) strains were isolated from various seafood (anchovy, sardine, sole, mullet, and octopus), or meat (pork, veal, beef, sheep, chicken, and turkey) products that were either fresh, or transformed by different traditional processes. Several methods were used in order to develop a rapid and reliable protocol for the direct identification of L. sakei. Amplified ribosomal DNA restriction analysis (ARDRA) classified the various isolates into 9 distinct groups. Search for the presence of the L. sakei specific katA gene indicated that all positive isolates were grouped in the same ARDRA group. Sequencing of 16S rDNA confirmed those isolates as L. sakei. Those 22 different L. sakei strains represent 25.6% of the total isolates, while other isolates found in the different ARDRA groups were tentatively ascribed to Lactobacillus plantarum, Lactococcus lactis/garviae, Enterococcus avium, Streptococcus parauberis, Hafnia alvei, Pediococcus pentosaceus, and Lactobacillus curvatus through 16S rDNA sequencing. A fast and reliable method to isolate and discriminate L. sakei from complex food environments is proposed. 相似文献
87.
Karim M. Abdellatif Roselyne Chotin-AvotAuthor VitaeHabib MehrezAuthor Vitae 《Microprocessors and Microsystems》2014
Recently, techniques have been invented to combine encryption and authentication into a single algorithm which is called Authenticated Encryption (AE). Combining these two security services in hardware produces smaller area compared to two separate algorithms. 相似文献
88.
89.
R Aboutaieb R Rabii A el Moussaoui A Joual I Sarf M el Mrini S Benjelloun 《Canadian Metallurgical Quarterly》1996,102(5-6):229-232
Kidney in ectopic position is dysplasic, and associated to other malformations. The advent of a lithiasis in these conditions rises questions about therapeutic options. We report on five observations of pelvic ectopic kidney with urinary lithiasis. Patients were aged from 16 to 42 years. Kidney was non functional in two cases, or with normal appearance sized 10 to 12 cm. We performed total nephrectomy in two cases, pyelolithotomy in the other cases. Surgical approach was subperitoneal via iliac route. A dismembered pyeloplasty was associated in one case. All patients did well. Radiologic control at 6 and 12 months showed no recurrence in a well functioning kidney. Surgical lithotomy is advocated as a treatment in urinary lithiasis affecting ectopic kidney. It is an easy procedure which permits correction of other associated malformations. 相似文献
90.