Effect of ETA receptor antagonists on cardiovascular responses induced by centrally administered sarafotoxin 6b: role of sympathetic nervous system

The present study was carried out to investigate the cardiovascular effects of centrally administered SRT6b in saline, BQ123 and BMS182874 pretreated male Sprague-Dawley rats, using a radioactive microsphere technique. SRT6b (100 ng, ICV) produced a transient increase (40%) in blood pressure at 5 min followed by a sustained decrease (-42%) at 30 and 60 min in control rats. Total peripheral resistance and heart rate were not significantly altered. Cardiac output increased (16%) at 5 min and decreased 30 and 60 min following SRT6b administration. Central venous pressure was not affected by SRT6b. Regional blood flow and vascular resistance did not change at 5 min following administration of SRT6b. However, a significant decrease in blood flow to the brain, heart, kidneys, liver, spleen, gastrointestinal tract and mesentery and pancreas was observed 30 and 60 min following administration of SRT6b in control (saline treated) rats. Pretreatment with ETA selective receptor antagonists, BQ123 (10 micrograms, ICV) or BMS182874 (50 micrograms, ICV) significantly attenuated the pressor and depressor effects of centrally administered SRT6b. SRT6b induced decrease in blood flow was completely blocked by pretreatment with BQ123 or BMS182874. ET-1 (100 ng, ICV) produced an increase followed by a decrease similar to SRT6b. Reserpine (5 mg/kg, IP) pretreatment attenuated the cardiovascular effects of ET-1. Role of sympathetic nervous system was determined by measuring splanchnic nerve activity. SRT6b when administered in the lateral cerebral ventricle did not produce any significant effect at 5 min, however, a significant decrease in sympathetic nerve activity was observed 30 min after its administration. It is concluded that centrally administered SRT6b produces significant changes in systematic and regional blood circulation which can be completely blocked by ETA receptor antagonist. The cardiovascular effects of centrally administered SRT6b appear to be mediated through the sympathetic nervous system.     

Profile design considerations for minimizing base transit time inSiGe HBT's

A unified closed form analytical model for base transit time of SiGe HBT's for uniform and exponential base dopant distributions with different Ge profiles in the base (e.g., box, trapezoidal, triangular) is reported. The model is subsequently used to study the design of Ge profile for different base doping profiles, including that of epitaxial base transistors. Consistent with the reported results, our unified model predicts that beyond a certain total Ge content, there is very little reduction in τ_b.SiGe. It is further demonstrated that the trapezoidal Ge profile with X_T~0.8W_B gives near optimal base transit time for all doping profiles considered. Our analysis shows that 1) for a given base width and intrinsic base resistance, the exponential base doping profile with Ge yields the least value of τ_{b, SiGe} and 2) for a given peak base doping concentration and the intrinsic base resistance, the uniform base doping with Ge gives minimum τ_{b, SiGe}. Also, the need for keeping the total base Ge content constant while optimizing the Ge profile in the base is emphasized by showing that a false minimum for τ_{b, SiGe} may appear if the total Ge content is not kept constant     

Bcl-2 deregulation leads to inhibition of sodium butyrate-induced apoptosis in human colorectal carcinoma cells

Epidemiological studies have linked dietary fiber to the prevention of human colorectal cancer and suggest that short chain fatty acids such as butyric acid, which is produced by fermentation of dietary fiber in the large intestine, may be an important mediator of the protective effects of fiber. We investigated the role of Bcl-2 deregulation on the sensitivity of colorectal carcinoma cells to undergo butyrate-induced apoptosis. Here we report an inverse relationship between the levels of Bcl-2 and the sensitivity of colorectal carcinoma cell lines to undergo apoptosis in response to butyrate. Overexpression of Bcl-2 in colorectal carcinoma DiFi cells resulted in suppression of butyrate-induced apoptosis and enhanced cell survival in response to butyrate. Butyrate-induced apoptosis was accompanied by inhibition of expression of a 30 kDa protein (p30, immunorecognized by anti-Bcl-2 mAb) and this cellular effect of butyrate was inhibited by Bcl-2 overexpression. These findings suggest that deregulation of Bcl-2 in human colorectal carcinoma cells confers resistance to induction of apoptosis by butyrate, a dietary micronutrient.     

A rule-based approach for robust clump splitting

This paper presents a robust rule-based approach for the splitting of binary clumps that are formed by objects of diverse shapes and sizes. First, the deepest boundary pixels, i.e., the concavity pixels in a clump, are detected using a fast and accurate scheme. Next, concavity-based rules are applied to generate the candidate split lines that join pairs of concavity pixels. A figure of merit is used to determine the best split line from the set of candidate lines. Experimental results show that the proposed approach is robust and accurate.     

Microwave-Assisted Synthesis of Fine Particle Oxides Employing Wet Redox Mixtures

Interaction of electromagnetic radiation with a physical mixture of metal nitrates and amides/hydrazides is observed to initiate high-temperature reactions, useful for realizing several high-temperature ceramic materials. A judicious choice of such redox mixtures undergoes exothermic reactions when they couple with microwave radiation. The coupling of electromagnetic radiation with metal salts and amides/hydrazides depends on the dielectric properties of the individual components in the reaction mixture. The approach has been used to prepare γ-Fe₂O₃, Fe₃O₄, MgCr₂O₄, α-CaCr₂O₄, and La_0.7Ba_0.3MnO₃.     

SYNTHESIS AND CHARACTERIZATION OF CONTROLLED SIZES: POLYSTYRENE LATEXES

Several parameters and their limitations to prepare monodisperse polystyrene particles were discussed. Polystyrene latexes B, C and D of diameter 129.8, 142.8 and 264.5 nm, respectively, were synthesized by emulsion polymerization method. The latex D is almost monodisperse with very low polydispersity of 0.005 while B and C are having polydispersity of 0.1837 and 0.1601. The particle size and particle size distribution of the latexes were determined by a TEM and Brookhaven particle size analyzer. It was observed that the particle size of the latexes decreased with increasing initiator (ammonium persulfate) or surfactant (SDS) concentrations. The surface area of the latexes were calculated by using BET equation on the basis of the amount of nitrogen gas adsorbed at the surface of the particles to form a monolayer. It was observed that the surface area of latex D is less than B and C because of the bigger size of the particles. The average molecular weights, zeta potentials and densities of the latexes were also presented.     

Free convection and mass transfer flow of non-newtonian fluid with thermal diffusion effect

An analytical study is performed to examine the thermal diffusion characteristics of free convection and mass transfer flow in a non-Newtonian fluid (Walters, 1962: liquid B') past a uniformly accelerated infinite vertical plate in the presence of thermal diffusion. The expression for the velocity field is obtained by the Laplace transform technique. The influence of the Soret number (a thermal diffusion parameter) on the velocity field is extensively discussed with the help of graphs.     

Testing of box-type solar cooker: Second figure of merit F2 and its variation with load and number of pots

The performance of a box-type solar cooker can be represented in terms of two figures of merit, F₁ and F₂. The second figure F₂ is a controlling factor in the sensible heating of a load. The present work validates F₂ by computing this figure from experimental data by two different procedures and comparing the results. An attempt has also been made to provide some guidelines for selecting a suitable temperature interval for determination of F₂. The results of some experiments to study the effect of number of pots and the load on F₂ have also been presented. It is recommended that for standardization tests should be conducted at full load.     

Overexpression of HER2 modulates bcl-2, bcl-XL, and tamoxifen-induced apoptosis in human MCF-7 breast cancer cells

Overexpression of HER2 in estrogen receptor (ER)-positive human breast tumors has been associated with resistance to endocrine therapy. Here we investigated the effects of HER2 on expression of apoptotic pathways and modulation of tamoxifen-induced apoptosis in ER-positive MCF-7 breast cancer cells. We report that HER2 overexpression in MCF-7 cells is accompanied by up-regulation of antiapoptotic Bcl-2 and Bcl-XL proteins and suppression of tamoxifen-induced apoptosis. In addition, human tumor cell lines that are both ER positive and overexpress HER2 also express enhanced levels of Bcl-2 compared to cells that are either ER positive or overexpress HER2 alone. Our findings suggest that possible deregulation of antiapoptotic Bcl-2 and Bcl-XL may be associated with the enhanced survival of HER2-overexpressing and ER-positive breast cancer cells treated with antiestrogens.