全文获取类型
收费全文 | 375篇 |
免费 | 25篇 |
专业分类
电工技术 | 2篇 |
化学工业 | 200篇 |
金属工艺 | 5篇 |
机械仪表 | 4篇 |
建筑科学 | 5篇 |
矿业工程 | 1篇 |
能源动力 | 3篇 |
轻工业 | 64篇 |
水利工程 | 4篇 |
无线电 | 23篇 |
一般工业技术 | 44篇 |
冶金工业 | 17篇 |
原子能技术 | 5篇 |
自动化技术 | 23篇 |
出版年
2024年 | 1篇 |
2023年 | 5篇 |
2022年 | 54篇 |
2021年 | 87篇 |
2020年 | 17篇 |
2019年 | 16篇 |
2018年 | 20篇 |
2017年 | 12篇 |
2016年 | 17篇 |
2015年 | 9篇 |
2014年 | 20篇 |
2013年 | 20篇 |
2012年 | 23篇 |
2011年 | 10篇 |
2010年 | 8篇 |
2009年 | 13篇 |
2008年 | 9篇 |
2007年 | 6篇 |
2006年 | 6篇 |
2005年 | 8篇 |
2004年 | 6篇 |
2003年 | 6篇 |
2002年 | 5篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 6篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 3篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1989年 | 1篇 |
排序方式: 共有400条查询结果,搜索用时 159 毫秒
61.
62.
O Kanauchi Y Fujiyama K Mitsuyama Y Araki T Ishii T Nakamura Y Hitomi K Agata T Saiki A Andoh A Toyonaga T Bamba 《Canadian Metallurgical Quarterly》1999,3(2):175-179
Germinated barley foodstuff (GBF) derived from the aleurone and scutellum fractions of germinated barley mainly consists of low-lignified hemicellulose and glutamine-rich protein. GBF improves the proliferation of intestinal epithelial cells and defecation, through the bacterial production of short chain fatty acids (SCFA), especially butyrate. In this study we investigated the mechanism of production of butyrate by microflora in humans and in vitro. Daily administration of 9 g GBF for 14 successive days significantly increased fecal butyrate content. Fecal Bifidobacterium and Eubacterium were also significantly increased by GBF administration in healthy volunteers. Ten anaerobic micro-organisms selected from intestinal microflora were cultured in vitro in the medium containing GBF as a sole carbon source (GBF medium). After a 3-day incubation, 7 strains (Bifidobacterium breve, Bifidobacterium longum, Lactobacillus acidophilus, Lactobacillus casei subsp. casei, Bacteroides ovatus, Clostridium butyricum, and Eubacterium limosum) lowered the medium pH producing SCFA. Eubacterium grown together with Bifidobacterium in GBF medium efficiently produced butyrate. On the other hand, GBF changed the intestinal microflora and increased probiotics such as Bifidobacterium in the intestinal tract. As a result, butyrate was produced by the mutual action of Eubacterium and Bifidobacterium. This butyrate is considered to enhance the proliferation of colonic epithelial cells. 相似文献
63.
Agata Winiarska Monika Knysak Katarzyna Nabrdalik Janusz Gumprecht Tomasz Stompr 《International journal of molecular sciences》2021,22(19)
The incidence of type 2 diabetes (T2D) has been increasing worldwide, and diabetic kidney disease (DKD) remains one of the leading long-term complications of T2D. Several lines of evidence indicate that glucose-lowering agents prevent the onset and progression of DKD in its early stages but are of limited efficacy in later stages of DKD. However, sodium-glucose cotransporter-2 inhibitors (SGLT2i) and glucagon-like peptide-1 receptor (GLP-1R) agonists were shown to exert nephroprotective effects in patients with established DKD, i.e., those who had a reduced glomerular filtration rate. These effects cannot be solely attributed to the improved metabolic control of diabetes. In our review, we attempted to discuss the interactions of both groups of agents with inflammation and oxidative stress—the key pathways contributing to organ damage in the course of diabetes. SGLT2i and GLP-1R agonists attenuate inflammation and oxidative stress in experimental in vitro and in vivo models of DKD in several ways. In addition, we have described experiments showing the same protective mechanisms as found in DKD in non-diabetic kidney injury models as well as in some tissues and organs other than the kidney. The interaction between both drug groups, inflammation and oxidative stress appears to have a universal mechanism of organ protection in diabetes and other diseases. 相似文献
64.
Bartłomiej Grygorcewicz Wioleta Chajęcka-Wierzchowska Adrian Augustyniak Agata Wasak Xymena Stachurska Paweł Nawrotek Barbara Dołęgowska 《Journal of Food Safety》2020,40(2):e12747
Shiga toxin-producing Escherichia coli is a common foodborne pathogen which transmission includes dairy products. In the search for novel biocontrol methods, bacteriophages have become important candidates for the eradication of foodborne pathogens. The aim of this study was to evaluate the bacteriophage-mediated reduction of E. coli O157:H7 in raw and filtered milk. Laboratory-scale tests showed that the bacteriophage ECPS-6 efficiently adsorbed to E. coli O157: H7 cells. Furthermore, ECPS-6 remained stable when heated at 70°C for 20 min and in a wide pH range from 3.0 to 11.0. The trials on contaminated milk were performed using filtered and unfiltered raw milk contaminated with 1 × 105 CFU × ml−1 of E. coli O157: H7. Bacteriophage was added at multiplicity of infection (MOI) 5 and 50. The ECPS-6 reached the highest lytic activity at MOI = 5 (25°C) which resulted in 4.74 Log10 CFU × ml−1 and 7.3 Log10 CFU × ml−1 reduction after 10 days for both tested strains, respectively. Under refrigerated conditions (4°C) the quantity of E. coli decreased to 1.5 Log10 CFU × ml−1 and 3.04 Log10 CFU × ml−1 for these strains, respectively. Usage of MOI = 50 for the treatment unfiltered milk led to the reduction of E. coli O157:H7 A-2 below the detection limit after 6 hr. 相似文献
65.
Marcin Grniak Dariusz L. Szlachetko Natalia Oldrzyska Aleksandra M. Naczk Agata Mieszkowska Lidia Boss Marek S. Zitara 《International journal of molecular sciences》2021,22(21)
The phylogeny of the genus Paphiopedilum based on the plastome is consistent with morphological analysis. However, to date, none of the analyzed nuclear markers has confirmed this. Topology incongruence among the trees of different nuclear markers concerns entire sections of the subgenus Paphiopedilum. The low-copy nuclear protein-coding gene PHYC was obtained for 22 species representing all sections and subgenera of Paphiopedilum. The nuclear-based phylogeny is supported by morphological characteristics and plastid data analysis. We assumed that an incongruence in nuclear gene trees is caused by ancestral homoploid hybridization. We present a model for inferring the phylogeny of the species despite the incongruence of the different tree topologies. Our analysis, based on six low-copy nuclear genes, is congruent with plastome phylogeny and has been confirmed by phylogenetic network analysis. 相似文献
66.
67.
68.
Ewelina Ziemliska Justyna Sobociska Anna
witkowska Aneta Hromada-Judycka Gabriela Traczyk Agata Malinowska Bianka
widerska Anna Mietelska-Porowska Anna Ciesielska Katarzyna Kwiatkowska 《International journal of molecular sciences》2021,22(23)
Palmitic acid (C16:0) is the most abundant saturated fatty acid in animals serving as a substrate in synthesis and β-oxidation of other lipids, and in the modification of proteins called palmitoylation. The influence of dietary palmitic acid on protein S-palmitoylation remains largely unknown. In this study we performed high-throughput proteomic analyses of a membrane-enriched fraction of murine liver to examine the influence of a palm oil-rich diet (HPD) on S-palmitoylation of proteins. HPD feeding for 4 weeks led to an accumulation of C16:0 and C18:1 fatty acids in livers which disappeared after 12-week feeding, in contrast to an accumulation of C16:0 in peritoneal macrophages. Parallel proteomic studies revealed that HPD feeding induced a sequence of changes of the level and/or S-palmitoylation of diverse liver proteins involved in fatty acid, cholesterol and amino acid metabolism, hemostasis, and neutrophil degranulation. The HPD diet did not lead to liver damage, however, it caused progressing obesity, hypercholesterolemia and hyperglycemia. We conclude that the relatively mild negative impact of such diet on liver functioning can be attributed to a lower bioavailability of palm oil-derived C16:0 vs. that of C18:1 and the efficiency of mechanisms preventing liver injury, possibly including dynamic protein S-palmitoylation. 相似文献
69.
Sferratore A Garnier J Billen G Conley DI Pinault S 《Environmental science & technology》2006,40(21):6630-6635
Dissolved silica (DSi) is believed to enter aquatic ecosystems primarily through diffuse sources by weathering. Point sources have generally been considered negligible, although recent reports of DSi inputs from domestic and industrial sources suggest otherwise. In addition, particulate amorphous silica (ASi) inputs from terrestrial ecosystems during soil erosion and in vegetation can dissolve and also be a significant source of DSi. We quantify here both point and diffuse sources of DSi and particulate ASi to the Seine River watershed. The total per capita point source inputs of Si (DSi + ASi) were found to be 1.0 and 0.8 g Si inhabitant(-1) d(-1) in raw and treated waters of the Achères wastewater treatment plant, in agreement with calculations based on average food intake and silica-containing washing products consumption. A mass balance of Si inputs and outputs for the Seine drainage network was established for wet and dry hydrological conditions (2001 and 2003, respectively). Diffuse sources of Si are of 1775 kg Si km(-2) y(-1) in wet conditions and 762 kg Si km(-2) y(-1) in dry conditions, with the proportion of ASi around 6%. Point sources of Si from urban discharge can contribute to more than 8% of the total Si inputs at the basin scale in hydrologically dry years. An in-stream retention of 6% of total inputs in dry conditions and 12% in wet conditions is inferred from the budget. 相似文献
70.
Laura Dipasquale Giuliana d'Ippolito Carmela Gallo Filomena Monica Vella Agata Gambacorta Gianluca Picariello Angelo Fontana 《International Journal of Hydrogen Energy》2012
Planktic diatoms are the largest primary producers in marine and freshwater habitats. Their dry biomass accumulates up to 50% of lipids and contains water-soluble β-1,3-glucans as major storage products. Because of the world-wide abundance of these photosynthetic protists, β-1,3-glucans may rival cellulose as the polysaccharide with the highest annual production on Earth. Here we show the feasibility of a simple and efficient process leading to bio-hydrogen by dark fermentation of microalgal biomass with the thermophilic bacterium Thermotoga neapolitana. Production of the biogas on minimum medium supplemented only with the extract of the diatom Thalassiosira weissflogii proved that algal biomass per se can serve as substrate for sustaining the biotechnological process with no requirement of any pretreatment and external integration of other nutrients. At the same time, lipids unused for the anaerobic production of the biogas, can be employed for production of bio-diesel, thus considerably increasing the economic potential of these renewable feedstocks. 相似文献