全文获取类型
收费全文 | 5833篇 |
免费 | 342篇 |
国内免费 | 46篇 |
专业分类
电工技术 | 74篇 |
综合类 | 20篇 |
化学工业 | 1633篇 |
金属工艺 | 124篇 |
机械仪表 | 252篇 |
建筑科学 | 155篇 |
矿业工程 | 4篇 |
能源动力 | 377篇 |
轻工业 | 557篇 |
水利工程 | 84篇 |
石油天然气 | 36篇 |
无线电 | 552篇 |
一般工业技术 | 1074篇 |
冶金工业 | 279篇 |
原子能技术 | 83篇 |
自动化技术 | 917篇 |
出版年
2024年 | 23篇 |
2023年 | 113篇 |
2022年 | 324篇 |
2021年 | 399篇 |
2020年 | 299篇 |
2019年 | 327篇 |
2018年 | 362篇 |
2017年 | 296篇 |
2016年 | 325篇 |
2015年 | 194篇 |
2014年 | 326篇 |
2013年 | 540篇 |
2012年 | 309篇 |
2011年 | 318篇 |
2010年 | 282篇 |
2009年 | 248篇 |
2008年 | 172篇 |
2007年 | 149篇 |
2006年 | 134篇 |
2005年 | 97篇 |
2004年 | 74篇 |
2003年 | 71篇 |
2002年 | 55篇 |
2001年 | 43篇 |
2000年 | 36篇 |
1999年 | 37篇 |
1998年 | 72篇 |
1997年 | 59篇 |
1996年 | 43篇 |
1995年 | 53篇 |
1994年 | 25篇 |
1993年 | 35篇 |
1992年 | 31篇 |
1991年 | 25篇 |
1990年 | 25篇 |
1989年 | 22篇 |
1988年 | 15篇 |
1987年 | 30篇 |
1986年 | 30篇 |
1985年 | 22篇 |
1984年 | 30篇 |
1983年 | 26篇 |
1982年 | 16篇 |
1981年 | 8篇 |
1980年 | 14篇 |
1979年 | 13篇 |
1978年 | 12篇 |
1977年 | 11篇 |
1976年 | 15篇 |
1975年 | 8篇 |
排序方式: 共有6221条查询结果,搜索用时 15 毫秒
981.
Gong Q Wang J Ahmad KM Csordas AT Zhou J Nie J Stewart R Thomson JA Rossi JJ Soh HT 《Analytical chemistry》2012,84(12):5365-5371
Many analytical techniques benefit greatly from the use of affinity reagent pairs, wherein each reagent recognizes a discrete binding site on a target. For example, antibody pairs have been widely used to dramatically increase the specificity of enzyme linked immunosorbent assays (ELISA). Nucleic acid-based aptamers offer many advantageous features relative to protein-based affinity reagents, including well-established chemical synthesis, thermostability, and low production cost. However, the generation of suitable aptamer pairs has posed a significant challenge, and few such pairs have been reported to date. To address this important challenge, we present multivalent aptamer isolation systematic evolution of ligands by exponential enrichment (MAI-SELEX), a technique designed for the efficient selection of aptamer pairs. In contrast to conventional selection methods, our method utilizes two selection modules to generate separate aptamer pools that recognize distinct binding sites on a single target. Using MAI-SELEX, we have isolated two groups of 2'-fluoro-modified RNA aptamers that specifically recognize the αV or β3 subunits of integrin αVβ3. These aptamers exhibit low nanomolar affinities for their targets, with minimal cross-reactivity to other closely related integrin homologues. Moreover, we show that these aptamer pairs do not interfere with each other's binding and effectively detect the target even in complex mixtures such as undiluted serum. 相似文献
982.
Y Xie D Ahmed MI Lapsley SC Lin AA Nawaz L Wang TJ Huang 《Analytical chemistry》2012,84(17):7495-7501
In this work we present an acoustofluidic approach for rapid, single-shot characterization of enzymatic reaction constants K(m) and k(cat). The acoustofluidic design involves a bubble anchored in a horseshoe structure which can be stimulated by a piezoelectric transducer to generate vortices in the fluid. The enzyme and substrate can thus be mixed rapidly, within 100 ms, by the vortices to yield the product. Enzymatic reaction constants K(m) and k(cat) can then be obtained from the reaction rate curves for different concentrations of substrate while holding the enzyme concentration constant. We studied the enzymatic reaction for β-galactosidase and its substrate (resorufin-β-D-galactopyranoside) and found K(m) and k(cat) to be 333 ± 130 μM and 64 ± 8 s(-1), respectively, which are in agreement with published data. Our approach is valuable for studying the kinetics of high-speed enzymatic reactions and other chemical reactions. 相似文献
983.
Aiman Al Alawin Omar Badran Ahmad Awad Yaser Abdelhadi Anwar Al-Mofleh 《Applied Solar Energy》2012,48(4):260-265
A solar chimney power plant system is theoretically designed for future erection in Jordan. Analytical analysis of the system is simulated by mathematical software. The actual values of solar irradiation in Jordan are used in the simulation to predict the power output of the solar chimney power plant. The output results of the maximum (inlet) values of velocity, pressure, and mass flow rate of air versus the chimney height variation are obtained. Furthermore, the electrical power output and the efficiency of chimney versus chimney height variation were determined. For a solar collector diameter of 40 m and a chimney diameter of 3.5 m, the maximum power output (85 kW) was obtained for a chimney height of 210 m. 相似文献
984.
Y.H. Yau H.F. Wong N. Ahmad 《International Journal of Heat and Mass Transfer》2012,55(11-12):2879-2888
The physical processes of heat transfer are typically complicated in the curing of rubber. In this study, the image plot and the plot of the temperature profile were obtained in such an environment, and then compared with the finite element method (FEM), which is used in finite element analysis (FEA) software. A good agreement was observed, and the difference was less than 1% when evaluating the different curing conditions. This confirms that the correct curing time can be determined by studying the temperature profile, which will help to avoid the deterioration of the mechanical properties of thick rubber products. 相似文献
985.
986.
Mohajeri SA Malaekeh-Nikouei B Sadegh H 《Drug development and industrial pharmacy》2012,38(5):616-622
Three different molecularly imprinted polymers (MIPs) for drug delivery of diclofenac in gastrointestinal tract were synthesized employing bulk polymerization method and their binding and release properties were studied in different pH values. Methacrylic acid (MAA), methacrylamide (MAAM) and 4-vinyl pyridine (4VP) were tested as functional monomers and ethylene glycole dimethacrylate (EDMA) was used as a cross-linker monomer in polymeric feed. Binding properties and imprinting factor (IF) of MIPs were studied in comparison with their non-imprinted ones (Blank) in organic and aqueous media. Diclofenac release in aqueous solvents at pH values of 1.5, 6.0 and 8.0, simulating gastrointestinal fluids, were also studied. The results indicated the specific binding of diclofenac to imprinted polymers. Duo to the stronger non-specific bounds in aqueous solutions, IF values decreased in water compared to acetonitrile as an organic medium. Our results proved that all polymers represented pH-responsive diclofenac delivery at above conditions. The data showed that imprinted polymer, prepared by MAA had superior properties, in comparison with other polymers, for minimum release (14%) of drug in gastric acid and maximum release (90%) in basic condition. The results indicated that diclofenac imprinted polymer could be used as a pH-responsive matrix in preparation of a new drug delivery system for diclofenac. 相似文献
987.
In this paper, we have discussed the application of new nonlinear recursive controllers on Josephson junction (JJ) chaotic systems. Controlling bifurcation as well as chaos has been rapidly advancing in the last decade. Thus, emphasis has been placed on control design techniques which result in prescribed nonlinear performance dynamics for practical controlled processes. This study has shown that a nonlinear recursive controller is effective in controlling an undesirable JJ chaotic behavior. In addition to that, the synchronization of two JJ chaotic nonlinear dynamical systems can be used to advantage in communication systems. In fact, communication security is promising through chaos. Using nonlinear control method, we have demonstrated that it is possible to achieve synchronization in electronic circuits such as JJ. The study showed the effectiveness of the designed controller in communication signal synchronization. For the purpose of verification and comparison, we designed a control signal based on master and slave concept. 相似文献
988.
Ahmad MR Nakajima M Kojima M Kojima S Homma M Fukuda T 《IEEE transactions on nanobioscience》2012,11(1):70-78
In this paper, single cells adhesion force was measured using a nanofork. The nanofork was used to pick up a single cell on a line array substrate inside an environmental scanning electron microscope (ESEM). The line array substrate was used to provide small gaps between the single cells and the substrate. Therefore, the nanofork could be inserted through these gaps in order to successfully pick up a single cell. Adhesion force was measured during the cell pick-up process from the deflection of the cantilever beam. The nanofork was fabricated using focused ion beam (FIB) etching process while the line array substrate was fabricated using nanoimprinting technology. As to investigate the effect of contact area on the strength of the adhesion force, two sizes of gap distance of line array substrate were used, i.e., 1 μm and 2 μm. Results showed that cells attached on the 1 μm gap line array substrate required more force to be released as compared to the cells attached on the 1 μm gap line array substrate. 相似文献
989.
990.
Muhammad Abdullah Jamil Ahmad Mazhar Mehmood H. Waqas M. Mujahid 《Composites Part B》2012,43(3):1564-1569
In the present study effect of deflocculants like P-Aminobenzoic Acid (PABA) and Cetyltrimethyl ammonium bromide (CTAB) on densification and hardness of 3 mol.% Yttria-stabilized ZrO2 (abridged as YSZ) + Al2O3 (whiskers or particulates) composite have been studied. Maximum hardness & density were achieved at 1 wt% of CTAB or PABA, while further addition (5, 10 and 15 wt%) had no significant affect on the aforementioned properties. It was also observed that alumina addition in form of particulates only improved the density while its addition in form of whiskers significantly increased the hardness of YSZ + alumina composite. The maximum hardness achieved was more than 14 GPa in case of sample containing alumina in form of whiskers. 相似文献