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51.
Y Tanaka K Fujii S Hübscher M Aso A Takazawa K Saito T Ota S Eto 《Canadian Metallurgical Quarterly》1998,41(8):1365-1377
OBJECTIVE: To clarify the role of heparan sulfate proteoglycan (HSPG) and chemokines in integrin-mediated T cell adhesion to endothelial cells in the synovium of patients with rheumatoid arthritis (RA). METHODS: Endothelial cells were purified from RA synovium. Expression of heparan sulfate, chemokines, and adhesion molecules on the endothelium was assessed by immunohistochemical analysis or flow cytometry. The effects of chemokines and heparan sulfate on T cell adhesion to RA endothelium were estimated with relevant antibodies and signaling inhibitors. Production of chemokines from synovial T cells was detected by Northern blotting and enzyme-linked immunosorbent assay. RESULTS: The endothelium in RA synovium highly expressed HSPG. The soluble form of chemokines, macrophage inflammatory protein 1beta (MIP-1beta), induced T cell adhesion to the endothelial cells. When MIP-lalpha and MIP-1beta were immobilized on RA endothelial cells, a more efficient integrin-mediated adhesion of T cells was induced compared with their soluble form. The induced T cell adhesion was reduced by pretreatment with either heparitinase, anti-MIP-lalpha antibody, or anti-MIP-lbeta antibody, indicating that these chemokines were bound to heparan sulfate on the cells. T cell adhesion was also inhibited by pertussis toxin, wortmannin, and cytochalasin B. MIP-lalpha and MIP-1beta were found on vessels in RA synovium in vivo, which were spontaneously produced from T cells purified from RA synovium. CONCLUSION: Endothelial cells in RA synovium characteristically express HSPG, which is involved in T cell integrin triggering by "posting" chemokines, which are produced by synovial T cells, and by "relaying" them to their receptors on T cells, which activate G protein-dependent phosphoinositide 3-kinase and actin-dependent integrin triggering. 相似文献
52.
T Saito T Nakahara Y Abe T Sugiura M Ogata Y Sugiyama T Watanabe M Honma C Hida T Yamamoto 《Canadian Metallurgical Quarterly》1998,50(12):1133-1141
BACKGROUND: This article presents results of the acute treatment phase of a 2-site study comparing cognitive behavioral group therapy (CBGT) and treatment with the monoamine oxidase inhibitor phenelzine sulfate for social phobia. METHODS: One hundred thirty-three patients from 2 sites received 12 weeks of CBGT, phenelzine therapy, pill placebo administration, or educational-supportive group therapy (an attention-placebo treatment of equal credibility to CBGT). The "allegiance effect," ie, the tendency for treatments to seem most efficacious in settings of similar theoretical orientation and less efficacious in theoretically divergent settings, was also examined by comparing responses to the treatment conditions at both sites: 1 known for pharmacological treatment of anxiety disorders and the other for cognitive behavioral treatment. RESULTs: After 12 weeks, phenelzine therapy and CBGT led to superior response rates and greater change on dimensional measures than did either control condition. However, response to phenelzine therapy was more evident after 6 weeks, and phenelzine therapy was also superior to CBGT after 12 weeks on some measures. There were few differences between sites, suggesting that these treatments can be efficacious at facilities with differing theoretical allegiances. CONCLUSIONS: After 12 weeks, both phenelzine therapy and CBGT were associated with marked positive response. Although phenelzine therapy was superior to CBGT on some measures, both were more efficacious than the control conditions. More extended cognitive behavioral treatment and the combination of modalities may enhance treatment effect. 相似文献
53.
Takashi Noji Yoji Koike Hideo Iwasaki Masatsune Kato Norio Kobayashi Yoshitami Saito 《Journal of Superconductivity and Novel Magnetism》1996,9(1):65-71
Anisotropie properties of the single crystal Pb2Sr2Ho0.5Ca0.5Cu3O8 have been investigated by measuring the electrical resistivity in theab-planeρ ab (H, θ,T), which depends on the angleθ between theab-plane and the magnetic-field direction, in various constant fieldsH perpendicular to the current direction. All the angle-dependent values ofρ ab (H, θ,T) at a constant temperature are scaled to be on one curve as a function of reduced field. The anisotropic parameter γ≡(m c * /m ab * )1/2 is estimated as 12–13, which is larger than that of YBa2Cu3O7 and much smaller than that of Bi2Sr2CaCu2O8. It has been concluded that the anisotropy does not always depend on the thickness of the blocking layer but seems to depend on the overlap of the electronic wave functions along thec-axis. Anisotropy in the pinning potential has also been discussed from the resistive tail in the temperature dependence ofρ ab (H,θ,T). 相似文献
54.
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56.
Kazuto Okazaki Tatsuya Ogiwara Dongshin Yang Kentaro Sakata Ken Saito Yoshifumi Sekine Fumio Uchikoba 《Artificial Life and Robotics》2011,16(2):229-233
This article presents the micro-electro-mechanical systems (MEMS) microrobot which demonstrates locomotion controlled by hardware
neural networks (HNN). The size of the microrobot fabricated by the MEMS technology is 4 × 4 × 3.5 mm. The frame of the robot
is made of silicon wafer, and it is equipped with a rotary-type actuator, a link mechanism, and six legs. The rotary-type
actuator generates rotational movement by applying an electrical current to artificial muscle wires. The locomotion of the
microrobot is obtained by the rotation of the rotary-type actuator. As in a living organism, the HNN realized robot control
without using any software programs, A/D converters, or additional driving circuits. A central pattern generator (CPG) model
was implemented as an HNN system to emulate the locomotion pattern. The MEMS microrobot emulated the locomotion method and
the neural networks of an insect with the rotary-type actuator, the link mechanism, and the HNN. The microrobot performed
forward and backward locomotion, and also changed direction by inputting an external trigger pulse. The locomotion speed was
0.325 mm/s and the step width was 1.3 mm. 相似文献
57.
Tree is a data structure used to express various objects such as semistructured data and genes. When objects are represented as trees, computing tree similarity is essential for pattern recognition and retrieval. This paper considers the noisy subsequence tree recognition problem whose purpose is to recognize the original tree, given its noisy subsequence tree. Previous research on this problem relied on constrained tree edit distance to measure the dissimilarity. However, the number of relabelings must be predetermined to compute it. This paper proposes a new dissimilarity measure for this problem. Our dissimilarity measure is obtained by counting the node edit operations included in the unit‐cost tree edit distance that contribute to the matching of node labels. The number of relabelings need not be specified to compute our dissimilarity measure. Moreover, our measure achieves more accurate recognition performance and faster execution speed than the constrained tree edit distance. Our measure is also useful to solve the tree inclusion problem which is the problem of deciding whether a tree includes another tree and shows the extent of approximate tree inclusion when a tree incompletely includes another tree. © 2011 Wiley Periodicals, Inc. 相似文献
58.
Kikuta K Tochigi N Saito S Shimoda T Morioka H Toyama Y Hosono A Suehara Y Beppu Y Kawai A Hirohashi S Kondo T 《Proteomics. Clinical applications》2010,4(5):560-567
Purpose : We aimed to identify novel chemotherapy responsiveness biomarkers for osteosarcoma (OS) by investigating the global protein expression profile of 12 biopsy samples from OS patients. Experimental design : Six patients were classified as good responders and six as poor responders, according to the Huvos grading system. The protein expression profiles obtained by 2‐D DIGE consisted of 2250 protein spots. Results : Among them, we identified 55 protein spots whose intensity was significantly different (Bonferroni adjusted p‐value<0.01) between the two patient groups. Mass spectrometric protein identification demonstrated that the 55 spots corresponded to 38 distinct gene products including peroxiredoxin 2 (PRDX 2). Use of a specific antibody against PRDX 2 confirmed the differential expression of PRDX 2 between good and poor responders, while PRDX 2 levels as measured by Western blotting correlated highly with their corresponding 2‐D DIGE values. The predictive value of PRDX 2 expression was further confirmed by examining an additional four OS cases using Western blotting. Conclusions and clinical relevance : These results establish PRDX 2 as a candidate for chemotherapy responsiveness marker in OS. Measuring PRDX 2 in biopsy samples before treatment may contribute to more effective management of OS. 相似文献
59.
Graphs with large spectral gap are important in various fields such as biology, sociology and computer science. In designing such graphs, an important question is how the probability of graphs with large spectral gap behaves. A method based on multicanonical Monte Carlo is introduced to quantify the behavior of this probability, which enables us to calculate extreme tails of the distribution. The proposed method is successfully applied to random 3-regular graphs and large deviation probability is estimated. 相似文献
60.
Yufeng Wang Akihiro Nakao Athanasios V. VasilakosJianhua Ma 《Computer Communications》2011,34(3):241-249
This paper thoroughly investigates the evolutionary dynamics of soft security mechanism, namely, reciprocity-based incentive mechanism, in P2P systems based on Evolutionary Game Theory (EGT). By soft security mechanism, it means social control mechanisms to overcome peers’ selfish (rational) behaviors, and encourage cooperation in P2P systems. Specifically, there exist three strategies in P2P systems: always cooperative (ALLC), always defect (ALLD) and reciprocator (R). Instead of existing work which take it for granted that, like ALLC users, R users did not bear any information-seeking cost, we assume small reciprocation cost, and study generalized mutation-selection dynamics. Our contributions are threefold: firstly, we prove and illustrate that, in a well-mixed P2P structure, ALLD is the only strict Nash equilibrium; secondly, we infer the specific condition under which evolution dynamics exhibits rock-scissors-paper oscillation in a structured P2P population. That is, the population cycles from ALLD to R to ALLC and back to ALLD; finally, we theoretically illustrate that the intensity of selection plays an important role in the evolutionary dynamics of P2P incentive mechanism. That is, when the intensity of selection is relatively weak and reciprocation cost limits to zero, the time average can be mostly concentrated on reciprocator. In brief, considering the existence of reciprocation cost and the small mutation in P2P incentive mechanisms, unlike existing work, it is impossible to simply achieve the “absolute cooperative” in P2P incentive mechanisms. On the other hand, stochastic evolution in P2P incentive mechanism with finite population and network structure still favor reciprocation. 相似文献