According to the release of authorization, several new transgenic soy crops are expected to be present in the harvest of soy beans. In contrast to earlier times, methods for the detection of these transgenic crops are available already in advance. However, these methods are mainly still inefficient single real-time PCR methods. To increase the efficiency of product control, a multiplex quantitative real-time PCR system was developed and characterized for the four new transgenic soy traits DP-356043-5, DP-305423-1, MON 87701 and BPS-CV127-9. It showed amplification efficiency, correlation and sensitivity similar to the single PCR systems applied therein. To evaluate the robustness, an appropriate testing scheme was developed and applied for the first time on this multiplex real-time PCR system. It showed the robust amplification of all analytes also in case where conditions were varied. This system allows relative multiplex quantification and/or delta–delta Ct method quantification and proofed the applicability in routine. 相似文献
Typically, 15–45% of the mixed liquor (sludge) in biological wastewater treatment plants (WWTPs) consists of inorganic (fixed) suspended solids. A portion of these inorganic compounds is grit (sand) originating from the influent. Grit accumulation impacts WWTP design and operating costs as these unbiodegradable solids reduce the effective treatment capacity of the bioreactor and other unit operations that must be sized to carry this material.The goal of this study was to characterize the performance of a hydrocyclone to selectively separate grit from activated sludge. Laboratory experiments were conducted with a 13 mm diameter Krebs hydrocyclone treating sludge from eight WWTPs. Reduced efficiencies of 17 ± 7% on fixed suspended solids and 9 ± 6% on volatile suspended solids were obtained. Grade efficiency curves enabled the development of a modified definition for cut size useful for this application. The characterization of hydrocyclone performance for grit removal from activated sludge will enable modelling of the process for integration into wastewater treatment simulators used for process performance prediction and design. 相似文献
We determined whether Allanblackia floribunda, a forest tree species that is valued for the fat extracted from its seeds, could be genetically improved for fruit/seed production by sampling 17–40 fruits from each of 70 trees that were distributed among four sites in wild stands. Fat was extracted from the seeds, and stearic and oleic acid content of the fat was estimated. Highly significant (P < 0.0001) between- and within-tree variation characterised fruits and seed characters. Between-site phenotypic variation was not significant. Stearic and oleic acid percentages in seed fat ranged from 44.16% to 66.12%, and from 24.95% to 48.42% per tree sample, respectively. Moderate repeatabilities were identified in fruit characters and mean seed mass. Moderate positive relationships were found between stearic and oleic acid percentages. Seed fat profiles were not found to vary with other fruit characters. Twenty “plus trees” were selected for breeding. 相似文献
Quinoline‐based oligoamide foldamers have been identified as a potent class of ligands for G‐quadruplex DNA. Their helical structure is thought to target G‐quadruplex loops or grooves and not G‐tetrads. We report a co‐crystal structure of the antiparallel hairpin dimeric DNA G‐quadruplex (G4T4G4)2 with tetramer 1 —a helically folded oligo‐quinolinecarboxamide bearing cationic side chains—that is consistent with this hypothesis. Multivalent foldamer–DNA interactions that modify the packing of (G4T4G4)2 in the solid state are observed. 相似文献
Thermal loading induces modifications of the precipitation microstructure of Al–Si–Cu–Mg alloys. This study focuses on the effect of deformation on precipitation microstructure during thermomechanical loadings. Several specimens were thermomechanically cycled while others were exposed to the same thermal cycles without any mechanical loading. The nature and morphological characteristics of the precipitation microstructure of the thermomechanically cycled specimens are compared to those of the thermally aged ones, using transmission electron microscopy (TEM), in order to assess the effect of deformation on the precipitation microstructure and especially on the kinetics of precipitate growth. The absence of any significant effect of superimposed straining during thermal cycling is discussed. Implications for the prevision of yield strength degradation during service operation are briefly presented.
The influence of polyesters end-groups on cyclic oligoester formation is investigated using a series of hydroxy-, carboxy- and methylester-terminated aliphatic polyesters, in the presence of various ester interchange catalysts. The presence of hydroxy end-groups is the preponderant factor on cyclodepolymerization kinetics. This indicates that the main reaction is the intramolecular hydroxy–ester interchange reaction between hydroxy end-groups and ester functions in the chain. Carboxy-ester and ester–ester interchanges play a minor role, as the cycle-chain equilibrium is reached only very slowly when carboxy- or ester-terminated polyesters are reacted. High temperature and the presence of tin catalysts are also favorable factors, while, as expected, dilution shifts the equilibrium toward the formation of high yields of cyclic oligoesters. A mechanism is proposed, based on the reverse of the “coordination-insertion” mechanism established for the ring-opening polymerization of lactones. 相似文献
8% Yttria‐stabilized zironcia (8YSZ) transparent ceramics have a wide technological applications. Segregation of the Y around the grain boundaries is favored by slow heating rate. The optimized sintering parameters helped in obtaining transparent ceramics of 8YSZ with a high percentage of cubic phase in addition to the presence of tetragonal phase. HRTEM was used to verify the grain growth suppression and to observe the presence of the cubic phase. The presence of cubic phase has suppressed the grain growth, which increased the transparency in the visible and infrared region without the addition of dopants or by utilizing high pressure. 相似文献