Aleksander Tank Falck  Bjarte Aarmo Lund  David Johansen  Trine Lund  Kirsti Ytrehus 《International journal of molecular sciences》2022,23(17)

The present study investigates infarct-reducing effects of blocking ischemia-induced opening of connexin43 hemichannels using peptides Gap19, Gap26 or Gap27. Cardioprotection by ischemic preconditioning (IPC) and Gap peptides was compared, and combined treatment was tested in isolated, perfused male rat hearts using function and infarct size after global ischemia, high-resolution respirometry of isolated mitochondrial and peptide binding kinetics as endpoints. The Gap peptides reduced infarct size significantly when given prior to ischemia plus at reperfusion (Gap19 76.2 ± 2.7, Gap26 72.9 ± 5.8 and Gap27 71.9 ± 5.8% of untreated control infarcts, mean ± SEM). Cardioprotection was lost when Gap26, but not Gap27 or Gap19, was combined with triggering IPC (IPC 73.4 ± 5.5, Gap19-IPC 60.9 ± 5.1, Gap26-IPC 109.6 ± 7.8, Gap27-IPC 56.3 ± 8.0% of untreated control infarct). Binding stability of peptide Gap26 to its specific extracellular loop sequence (EL2) of connexin43 was stronger than Gap27 to its corresponding loop EL1 (dissociation rate constant K_d 0.061 ± 0.004 vs. 0.0043 ± 0.0001 s⁻¹, mean ± SD). Mitochondria from IPC hearts showed slightly but significantly reduced respiratory control ratio (RCR). In vitro addition of Gap peptides did not significantly alter respiration. If transient hemichannel activity is part of the IPC triggering event, inhibition of IPC triggering stimuli might limit the use of cardioprotective Gap peptides.  相似文献   

    

Robert Rusinek  Aleksander Siger  Marzena Gawrysiak-Witulska  Ewa Rokosik  Urszula Malaga-Toboła  Marek Gancarz 《International Journal of Food Science & Technology》2020,55(5):2161-2170

The article presents an attempt to use an electronic nose together with a new three-parameter method for generation of a digital smellprint in order to specify the mode of processing of rapeseed based on the analysis of volatile compounds contained in cold-pressed rapeseed oil. Prior to the pressing process, the seeds were roasted or improperly stored to obtain oil samples with varied technological quality. The quality of pressed oils was evaluated by determination of the acid value. Furthermore, changes in oil colour were assessed with the use of an imaging colorimeter. Volatile compounds were determined with the use of gas chromatography and an electronic nose with a metal oxide semiconductor (MOS) sensor matrix. It was found that the mode of seed pre-treatment before pressing did not change the colour of the oil significantly. However, it influenced the profile of volatile organic compounds and changed their proportions. Ketones represented the largest proportion of volatile compounds determined for roasted samples and those pressed from seeds moistened up to 25% (w.b.). Alcohols dominated in samples moistened up to 10 and 12%, terpenes were the dominant volatile compounds in samples roasted at 140 °C, and other volatile compounds dominated in samples moistened up to 10 and 20% (w.b.). In turn, esters and aromatic compounds accounted for the lowest proportion in the analysed samples. The results shown by the electronic nose were correlated with the presence of particular groups of volatile compounds in rapeseed oil.  相似文献   

    

Giovanni Vinai  Federico Motti  Valentina Bonanni  Aleksander Yu. Petrov  Stefania Benedetti  Christian Rinaldi  Mattia Stella  Damiano Cassese  Stefano Prato  Matteo Cantoni  Giorgio Rossi  Giancarlo Panaccione  Piero Torelli 《Advanced Electronic Materials》2019,5(7)

Converse magnetoelectric coupling in artificial multiferroics is generally modeled through three possible mechanisms: charge transfer, strain mediated effects or ion migration. Here the role played by electrically controlled morphological modifications on the ferromagnetic response of a multiferroic heterostructure, specifically Fe_xMn_1−x ferromagnetic films on piezoferroelectric PMN‐PT [001] substrates, is discussed. The substrates present, in correspondence to electrical switching, fully reversible morphological changes at the surface, to which correspond reproducible modifications of the ferromagnetic response of the Fe_xMn_1−x films. Topographic analysis by atomic force microscopy shows the formation of surface cracks (up to 100 nm in height) upon application of a sufficiently high positive electric field (up to 6 kV cm⁻¹). The cracks disappear after application of negative electric field of the same magnitude. Correspondingly, in operando X‐ray magnetic circular dichroic spectroscopy at Fe edge in Fe_xMn_1−x layers and micro‐MOKE measurements show local variations in the intensity of the dichroic signal and in the magnetic anisotropy as a function of the electrically driven morphological state. This morphologic parameter, rarely explored in literature, directly affects the ferromagnetic response of the system. Its proof of electrically reversible modification of the magnetic response adds a new possibility in the design of electrically controlled magnetic devices.  相似文献   

    

Julio Aleman  Sunil K. George  Samuel Herberg  Mahesh Devarasetty  Christopher D. Porada  Aleksander Skardal  Graa Almeida‐Porada 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(43)

Human hematopoietic niches are complex specialized microenvironments that maintain and regulate hematopoietic stem and progenitor cells (HSPC). Thus far, most of the studies performed investigating alterations of HSPC‐niche dynamic interactions are conducted in animal models. Herein, organ microengineering with microfluidics is combined to develop a human bone marrow (BM)‐on‐a‐chip with an integrated recirculating perfusion system that consolidates a variety of important parameters such as 3D architecture, cell–cell/cell–matrix interactions, and circulation, allowing a better mimicry of in vivo conditions. The complex BM environment is deconvoluted to 4 major distinct, but integrated, tissue‐engineered 3D niche constructs housed within a single, closed, recirculating microfluidic device system, and equipped with cell tracking technology. It is shown that this technology successfully enables the identification and quantification of preferential interactions—homing and retention—of circulating normal and malignant HSPC with distinct niches.  相似文献   

    

Nicolas Godard  Sebastjan Glin&#x;ek  Aleksander Matav  Vid Bobnar  Emmanuel Defay 《Advanced Materials Technologies》2019,4(2)

Inkjet printing is currently gaining momentum as a low‐cost and eco‐friendly additive manufacturing technique for electronics. Patterning of functional metal oxide thin films on high surface energy substrates via inkjet printing remains a challenge and requires assistance from lithographic processes. This work unveils a novel process for lithography and etching‐free patterning of lead zirconate titanate (PZT) films on pristine platinized silicon through the use of inkjet‐printed alkanethiolate‐based templates. The technique requires neither lithography nor etching, respectively, before and after PZT printing. The described process allows for feature sizes in the sub‐100 µm range with control over the thickness of the final film. Inkjet‐printed PZT displays typical ferroelectric and piezoelectric properties of solution‐derived thin films, with remnant polarization 13 µC cm⁻², coercive field 58 kV cm⁻¹, permittivity 900, dielectric losses 0.07, and effective longitudinal piezoelectric coefficient 50 pm V⁻¹.  相似文献   

    

Kirill Savostyanov  Nikolay Murashkin  Alexander Pushkov  Ilya Zhanin  Elkhan Suleymanov  Mariya Akhkiamova  Olga Shchagina  Elena Balanovska  Roman Epishev  Aleksander Polyakov  Andrey Fisenko 《International journal of molecular sciences》2022,23(22)

The pathogenic variants of genes encoding proteins, participating in the formation and functioning of epidermis and dermo-epidermal junctions, create a large variety of clinical phenotypes from: small localized to severe generalized dermatitis, as well as early, or even, prenatal death due to extensive epidermis loss. The diagnostic panel in this study was developed for the purposes of identifying these pathogenic genetic variants in 268 Russian children, who possessed the epidermolysis bullosa symptom complex in a selection of 247 families. This panel included the targeted areas of 33 genes, which are genetic variants that can lead to the development of the phenotype mentioned above. The usage of next generation sequencing allowed the revelation of 192 various altered alleles (of which 109 alleles were novel, i.e., had not been described previously). In addition, it allowed the definition of the genetic variants that are both typical for most of the examined children and for the separate ethnic groups inhabiting modern Russia. We found that the most characteristic mutations for the Dargin and Chechen ethnic groups are the c.3577del deletion in the COL7A1 gene and the c.2488G>A missense mutation in the COL17A1 gene, respectively. In addition, the study of haplotypes of microsatellite markers, which we managed to conduct in the Dargin population, confirmed the presence of the founder effect.  相似文献   

    

Maria Szubert  Anna Nowak-Glück  Daria Doma&#x;ska-Senderowska  Boena Szyma&#x;ska  Piotr Sowa  Aleksander Rycerz  Jacek R. Wilczy&#x;ski 《International journal of molecular sciences》2022,23(9)

Micro-RNAs expression can vary between different forms of endometriosis, but data on miRNA expression in cesarean scar endometriosis is lacking. The present study is comprised of 30 patients with endometriosis in the cesarean scar (scar endometriosis, SE), 14 patients with deep infiltrating endometriosis (DIE), 47 patients with endometrioma (ovarian endometrial cyst, OE), and 33 patients with healthy ovarian tissue as the control group (CG). In the initial experiment to identify possible dysregulated miRNAs, the levels of 754 miRNAs in formalin-fixed paraffin-embedded tissue (FFPE) samples from OE, high-grade ovarian cancer, endometrioid ovarian cancer, and CG were measured. We identified seven potentially dysregulated miRNAs: miR-1-3p, miR-31-3p, miR-125b-1-3p, miR-200b-3p, miR-548d, miR-502, and miR-503. We then examined the expression profiles of each of these miRNAs individually in the SE, DIE, OE, and CG FFPE samples using RT-qPCR. miR-31-3p had significantly higher levels of expression and miR-125b-1-3p had significantly lower levels of expression in SE compared to the controls. Overall, the higher expression levels of miR-31-3p and the lower expression levels of miR-125b-1-3p are consistent with the benign nature of SE. Importantly, the results of the present study demonstrate the possibility of using miRNA to monitor the risk of malignant transformation of endometriosis tissue.  相似文献   

    

Wojciech Dyga  Aleksander Obtulowicz  Tomasz Mikolajczyk  Anna Bogdali  Pawel Dubiela  Krystyna Obtulowicz 《International journal of molecular sciences》2022,23(18)

Background: Hereditary angioedema (HAE) is a rare, genetic disease caused by the decreased level or function of the C1 inhibitor. The primary mediator of symptoms in HAE is bradykinin acting through its two receptors, namely receptors 1 (BR1) and 2 (BR2). Although BR2 is well characterized, the role of BR1 remains unclear. Objective: To study the role of bradykinin receptors 1 (BR1) in the etiopathogenesis of HAE. Methods: A total of 70 individuals, 40 patients with HAE, and 30 healthy subjects were recruited to the study. HAE was diagnosed in accordance with the international guideline. The level of bradykinin receptors was determined in populations of CD3⁺, CD4⁺, CD8⁺, and CD14⁺⁺CD16⁻, CD14⁺⁺CD16⁺ monocytes. In addition, the level of disease activity-specific markers was measured. Results: There were statistically significant differences in the subpopulation of lymphocytes and monocytes between patients with HAE compared to healthy subjects. The level of BR1 and BR2 on PBMCs was comparable in healthy subjects and HAE patients during remission with significant overexpression of both receptors, triggered by HAE attack. Moreover, a significant increase in TNF-alpha and IL-1 plasma levels was observed among HAE patients. Conclusions: BR1 expression may play an important role in the pathomechanism of HAE.  相似文献   

    

Patryk Rybczynski  Aleksander Smolarkiewicz-Wyczachowski  Jaroslaw Piskorz  Szymon Bocian  Marta Ziegler-Borowska  Dariusz K&#x;dziera  Anna Kaczmarek-K&#x;dziera 《International journal of molecular sciences》2021,22(13)

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Aleksander Struga&#x;a  Jakub Jagielski  Karol Kamel  Grzegorz Nowaczyk  Marcin Radom  Marek Figlerowicz  Anna Urbanowicz 《International journal of molecular sciences》2021,22(6)

Virus-like particles (VLPs), due to their nanoscale dimensions, presence of interior cavities, self-organization abilities and responsiveness to environmental changes, are of interest in the field of nanotechnology. Nevertheless, comprehensive knowledge of VLP self-assembly principles is incomplete. VLP formation is governed by two types of interactions: protein–cargo and protein–protein. These interactions can be modulated by the physicochemical properties of the surroundings. Here, we used brome mosaic virus (BMV) capsid protein produced in an E. coli expression system to study the impact of ionic strength, pH and encapsulated cargo on the assembly of VLPs and their features. We showed that empty VLP assembly strongly depends on pH whereas ionic strength of the buffer plays secondary but significant role. Comparison of VLPs containing tRNA and polystyrene sulfonic acid (PSS) revealed that the structured tRNA profoundly increases VLPs stability. We also designed and produced mutated BMV capsid proteins that formed VLPs showing altered diameters and stability compared to VLPs composed of unmodified proteins. We also observed that VLPs containing unstructured polyelectrolyte (PSS) adopt compact but not necessarily more stable structures. Thus, our methodology of VLP production allows for obtaining different VLP variants and their adjustment to the incorporated cargo.  相似文献