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11.
A simple and rapid methodology for the preparation of single-cell suspension from animal tissues is described. It uses a device called a "tissue press" to mince the tissue into small pieces of desired sizes which are then dissociated into single cells using a pipette. The device is inexpensive and the method economizes time and labor. An example of its use in DNA double-strand breaks study, using microgel electrophoresis, is described.  相似文献   
12.
Every quadratic programming problem with a mix of continuous and binary variables can be equivalently reformulated as a completely positive optimization problem, that is, a linear optimization problem over the convex but computationally intractable cone of completely positive matrices. In this paper, we focus on general inner approximations of the cone of completely positive matrices on instances of completely positive optimization problems that arise from the reformulation of mixed binary quadratic programming problems. We provide a characterization of the feasibility of such an inner approximation as well as the optimal value of a feasible inner approximation. In particular, our results imply that polyhedral inner approximations are equivalent to a finite discretization of the feasible region of the original completely positive optimization problem. Our characterization yields, as a byproduct, an upper bound on the gap between the optimal value of an inner approximation and that of the original instance. We discuss the implications of this error bound for standard and box-constrained quadratic programs as well as general mixed binary quadratic programs with a bounded feasible region.  相似文献   
13.
Kara  Memduh  Ak  Safa  Uyaner  Mesut  Gunoz  Alper  Kepir  Yusuf 《Applied Composite Materials》2021,28(5):1567-1587

Chemical transmission lines, petroleum and natural gas lines, pressure vessels, and pipes used in thermal facilities are expected to maintain their mechanical properties for many years without being damaged and not to be corroded in working conditions. The composite materials are the right candidate for these harsh conditions due to their superior properties. Reinforcement of nanoadditives to composite materials improves both the mechanical properties and the resistance to environmental conditions, thereby increasing the lifetime. In this study, multi-walled carbon nanotube (MWCNT) reinforced [±?55°] carbon fiber/epoxy composite pipes produced with filament wound method were used. It was hydrothermally aged in 80 °C distilled water for 1, 2, 3 weeks in order to examine the effect of environmental conditions. In order to investigate its resistance against loads that may occur in working conditions, ring tensile tests (ASTM D 2290–16 procedure A), and low-velocity impact tests at 5, 10, 15 J, energy levels were carried out. The effect of hydrothermal aging on neat and MWCNT added epoxy composite had been examined by considering the aging period. Consequently, the impact resistance of neat and MWCNT added samples decreased with the aging process. Besides, tangential tensile strength loss was 17% in MWCNT reinforced sample and 13% in the neat sample.

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Most preset response surface methodology (RSM) designs offer ease of implementation and good performance over a wide range of process and design optimization applications. These designs often lack the ability to adapt the design on the basis of the characteristics of application and experimental space so as to reduce the number of experiments necessary. Hence, they are not cost‐effective for applications where the cost of experimentation is high or when the experimentation resources are limited. In this paper, we present an adaptive sequential response surface methodology (ASRSM) for industrial experiments with high experimentation cost, limited experimental resources, and high design optimization performance requirement. The proposed approach is a sequential adaptive experimentation approach that combines concepts from nonlinear optimization, design of experiments, and response surface optimization. The ASRSM uses the information gained from the previous experiments to design the subsequent experiment by simultaneously reducing the region of interest and identifying factor combinations for new experiments. Its major advantage is the experimentation efficiency such that for a given response target, it identifies the input factor combination (or containing region) in less number of experiments than the classical single‐shot RSM designs. Through extensive simulated experiments and real‐world case studies, we show that the proposed ASRSM method outperforms the popular central composite design method and compares favorably with optimal designs. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   
16.
In the present work, six new kinds of building composite PCMs (BCPCMs), PS/octadecane, BC/octadecane, PS/CA–MA, BC/CA–MA, PS/PEG1000, and BC/PEG1000 composites, were prepared by using vacuum impregnation method. The maximum percent of PCM in the composites was assigned to be 12, 13, 18, 23, 30, and 42 wt%, respectively. The form‐stable BCPCMs were characterized using SEM, FT‐IR, DSC, and TG analysis techniques. The characterization results showed the existence of homogenous dispersion of the PCM into the PBM matrixes. The DSC measurements indicated that the melting temperatures of the form‐stable BCPCMs are in the range of 20–33°C while they have latent heats of melting in the range of about 28–55 J/g. These results make them promising BCPCMs for low temperature‐passive TES applications in buildings. Thermal cycling test indicated that the prepared BCPCMs have good thermal reliability and chemical stability. TG analysis proved that the prepared BCPCMs have good thermal durability. In addition, the thermal conductivity of BCPCMs was enhanced considerably by addition of expanded graphite (EG). The improvement in thermal conductivity of the BCPCMs caused appreciably reduction in their melting times. Copyright © 2014 John Wiley & Sons, Ltd.  相似文献   
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This study examined measurement invariance of the Gifted Rating Scales—School Form (GRS–S) across the United States, Puerto Rico, China, South Korea, and Turkey, using multigroup confirmatory factor analysis. A total of 1,817 students were rated by 287 teachers using either translated versions of GRS–S or the original English GRS–S. Results indicate a similar factor structure for the GRS–S across the five locations; six factors with each of the 72 items equivalently loaded to the same latent variable across groups. The metric invariance test and the factor variance and covariance invariance tests reveal that the patterns of factor loadings and the factor variances and covariances are invariant across the five groups. Moreover, the scalar invariance test indicates that item means are equivalent across the groups. These results suggest that the GRS–S has intercultural utility and can be similarly interpreted. Implications and limitations of the present research for gifted identification are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)  相似文献   
19.
In this study, ~?3.5 µm thick multilayer titanium alumina nitride (TiAlN), alumina titanium nitride (AlTiN), and alumina chromium nitride (AlCrN) coatings were deposited on the H13 steel surface by cathodic arc physical vapor deposition (CAPVD) method. The tribological performance of the coatings was evaluated by a tribometer at boundary lubrication condition. Then, coating surfaces were observed by optical microscope, optical profilometer, and atomic force microscope to evaluate the morphological changes, wear volumes, and tribofilm thickness. Also, scanning electron microscopy (energy dispersive X-ray) and X-ray photoelectron spectrometry analyses were applied to coating surfaces for the tribochemical evolution of the tribofilm. Results showed that AlCrN coating performed the best tribological behavior at boundary lubricated condition, when compared to TiAlN and AlTiN coatings and it can be used as a wear resistant cam tappet coating in internal combustion engines.  相似文献   
20.
Melt rheology and crystallization behavior of polyamide 6 (PA 6) and microcrystalline cellulose (MCC) composites were systematically studied in this research. The incorporation of MCC into the PA 6 matrix resulted in higher complex viscosities (|η*|), storage modulus (G′), and shear viscosities than those of neat PA 6, especially at low frequencies. The orientation of rigid molecular chains in the composites introduced by the addition of MCC induced a strong shear thinning behavior with an increase in MCC loading. The non‐isothermal crystallization kinetics of PA 6 and MCC composites were investigated by differential scanning calorimetry. The Avrami and Tobin model were applied to describe the process of non‐isothermal crystallization and to determine the crystallization parameters of the composites. Analysis of the crystallization kinetics indicated that the Avrami (na) and Tobin exponent (nt) was altered by the MCC. It was also found that the Avrami and Tobin equations fit the empirical data well. POLYM. ENG. SCI., 54:739–746, 2014. © 2013 Society of Plastics Engineers  相似文献   
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