Effect of beta-adrenergic agonists on paracellular width and fluid flow across outflow pathway cells

PURPOSE: To determine whether the adrenergic agonists epinephrine and isoproterenol regulate fluid flow across endothelial cells cultured from the human aqueous outflow pathway and to evaluate associated cellular mechanisms. METHODS: Confluent monolayers of human trabecular meshwork (TM) or Schlemm's canal endothelial (SCE) cells were grown on porous filter supports. The monolayers were perfused with media while fluid flow, expressed as hydraulic conductivity (HC = microl/min/mm Hg/cm2), was continuously measured in preparations treated with isoproterenol, epinephrine, or control medium. Morphometric ultrastructural methods were used to measure the area occupied by the intercellular space and by each cell. RESULTS: SCE cells and TM cells exposed to isoproterenol or epinephrine responded with an increase in transendothelial fluid flow. Dose-response curves for both adrenergic agonists showed that HC increased linearly as a function of the log of the isoproterenol and epinephrine concentration. At 10(-4) M isoproterenol, the HC increased threefold, and threshold conditions were reached at 10(-9) M. The increase in HC was apparent after isoproterenol had been applied for 1 hour, reached a peak in 3 to 4 hours, and declined gradually to return to baseline conditions in 10 to 12 hours. Morphometric analyses showed that the area occupied by the intercellular space increased fourfold when isoproterenol was used at 10(-4) M, whereas the cell area decreased as a function of the concentration of adrenergic agonist. Epinephrine's effects on HC and cell morphology were blocked by pretreatment with equimolar concentrations of the nonselective beta-blocker, timolol. CONCLUSIONS: Epinephrine and isoproterenol increase flow through the paracellular pathway of SCE and TM cells through a beta-receptor mediated response that widens the intercellular space and reduces cell area. These findings support the hypothesis that epinephrine decreases the intraocular pressure in glaucoma therapy by promoting fluid flow across the SCE and TM cells lining tissues of the major aqueous outflow pathway.     

The development of a course in basic physical examination skills

Basic clinical skills of most medical school undergraduates continue unobserved and deficiencies have been detected in a significant number of physicians during residency. Nevertheless, our health care system is calling for competent graduates with solid basic clinical skills and a larger representation of qualified generalists in the increasingly important managed care environment. The need for a better introduction to Clinical Skills course was identified by students and clinical faculty at Ponce School of Medicine. In response to these concerns a new curriculum was developed with clear objectives, effective instructional strategies, and performance-based evaluation, with adult learning principles as its framework. The musculoskeletal examination unit of the curriculum was pilot tested and the course evaluation strategies revealed satisfaction with objectives, instructional and evaluation strategies, as well as improved confidence, and sense of usefulness for the learned skills. A curriculum in basic clinical skills that incorporates adult learning principles with solid instructional strategies can increase the confidence and skills of the learners and should lead to improved outcomes.     

Steel vs. plastics: The competition for light-vehicle fuel tanks

As weight and cost savings drive changes in performance criteria for automotive materials, original equipment manufacturers (OEMs) are taking a harder look at the historically terne-coated steel used for gas tanks. This article compares steel and plastic for gas tank uses according to performance attributes and a competitive analysis. Legislation issues and current OEM activity are included.     

Harmonic distortion analysis using an improved charge sheet model for PD SOI MOSFETs

This work presents an Improved Charge Sheet compact Model (ICSM) especially valuable for distortion analysis, where precise calculation of derivatives of at least third order is required. A new expression for the charge is used in the calculation of the current. Vertical electric field, mobility and DIBL are represented using previously reported for other purposes more precise expressions. The very good agreement obtained between experimental PD SOI MOSFETs with channel lengths from 0.32 to 10 μm and modeled currents, derivatives and distortion figures is shown.     

Parallel Inversion of Sparse Matrices.

This paper presents a parallel algorithm for obtaining the inverse of a large, nonsingular symmetric matrix A of dimension nxn. The inversion method proposed is based on the triangular factors of A. The task of obtaining the "sparse inverse' of A is represented by a directed acyclic graph. The relation between the triangulation graph and the sparse inversion graph is given. The algorithm and the graph for the full inversion of A is also given. It is shown that for any sparse symmetric matrix, and assuming enough processors are available, the full inverse of the matrix can be calculated in the same amount of time as the sparse inverse. For ideally sparse matrices (such as tridiagonal matrices) the order of computation required in both cases is of order log2n. For full matrices the order of computation is n log2n. Claims are substantiated using test data from several power systems.     

In-line interferometer for direction-sensitive displacement measurements by optical feedback detection

We demonstrate a compact in-line interferometer for direction-sensitive displacement measurement by optical feedback detection with a semiconductor laser (SL) light source. Two reflected beams from a semitransparent reference mirror and a reflecting test object interfere in the SL medium, causing a variation in its output power. The reference mirror is located between the SL output facet and the test object. The performance of the interferometer is investigated numerically and experimentally to determine its optimal operating conditions. We have verified the operating conditions where the behavior of the SL output power profile could indicate accurately the displacement magnitude and direction of the moving test object. The profile behavior is robust against variations in optical feedback and scale of the interferometer configuration.     

Distribution of max-log metrics for QAM-based BICM in fading channels

In this letter we derive closed-form expressions for the probability density functions (PDFs) of the bits' reliability metrics (L-values) in bit-interleaved coded modulation (BICM) transmission over fully-interleaved fading channels. The expressions are valid for the relevant case of quadrature amplitude modulation (QAM) with Gray mapping when the metrics are calculated via the so-called max-log approximation. Using the developed expressions, the performance of coded BICM transmissions is efficiently evaluated, i.e., without resorting to otherwise required two-dimensional numerical integration. The BICM capacity for different fading channels and constellation sizes is also evaluated.     

Race differences in nicotine dependence in the Collaborative Genetic study of Nicotine Dependence (COGEND)

In a general population sample from the Detroit site of the Collaborative Genetic Study of Nicotine Dependence (COGEND), we tested Black-White differences in nicotine dependence, measured by "how soon after wake-up the smokers smoked their first cigarette (time to first cigarette TTFC)", and its relationship with number of cigarettes per day (CPD). Analysis was conducted on respondents who have smoked > or =100 cigarettes in lifetime and were current smokers (n = 1,442; 1,087 Whites and 355 Blacks). In univariate analysis, we found no significant race differences on time to first cigarette (chi2 = 2.9, p value = 0.41), but significant race differences on CPD (chi2 = 154.3, p<.01), both categorized by the Fagerstr?m Test of Nicotine Dependence (FTND) cutoffs. We estimated the probability of TTFC < or =30 min given CPD using probit models. The interactions between race and CPD indicated significant differences in dependence at various levels of CPD. The same probability of nicotine dependence was associated with smaller increments in CPD for Blacks than for Whites. The data support the hypothesis that the relationship between CPD and nicotine dependence as reflected in relapse varies by race, and that Black smokers are dependent at lower levels of CPD than Whites.     

Use of UV light for the inactivation of Listeria monocytogenes and lactic acid bacteria species in recirculated chill brines

Ready-to-eat meat products have been implicated in several foodborne listeriosis outbreaks. Microbial contamination of these products can occur after thermal processing when products are chilled in salt brines. The objective of this study was to evaluate UV radiation on the inactivation of Listeria monocytogenes and lactic acid bacteria in a model brine chiller system. Two concentrations of brine (7.9% [wt/wt] or 13.2% [wt/wt]) were inoculated with a approximately 6.0 log CFU/ml cocktail of L. monocytogenes or lactic acid bacteria and passed through a UV treatment system for 60 min. Three replications of each bacteria-and-brine combination were performed and resulted in at least a 4.5-log reduction in microbial numbers in all treated brines after exposure to UV light. Bacterial populations were significantly reduced after 5 min of exposure to UV light in the model brine chiller compared with the control, which received no UV light exposure (P < 0.05). The maximum rate of inactivation for both microorganisms in treated brines occurred between minutes 1 and 15 of UV exposure. Results indicate that in-line treatment of chill brines with UV light reduces the number of L. monocytogenes and lactic acid bacteria.