RAB10 Interacts with ABCB4 and Regulates Its Intracellular Traffic

ABCB4 (ATP-binding cassette subfamily B member 4) is an ABC transporter expressed at the canalicular membrane of hepatocytes where it ensures phosphatidylcholine secretion into bile. Genetic variations of ABCB4 are associated with several rare cholestatic diseases. The available treatments are not efficient for a significant proportion of patients with ABCB4-related diseases and liver transplantation is often required. The development of novel therapies requires a deep understanding of the molecular mechanisms regulating ABCB4 expression, intracellular traffic, and function. Using an immunoprecipitation approach combined with mass spectrometry analyses, we have identified the small GTPase RAB10 as a novel molecular partner of ABCB4. Our results indicate that the overexpression of wild type RAB10 or its dominant-active mutant significantly increases the amount of ABCB4 at the plasma membrane expression and its phosphatidylcholine floppase function. Contrariwise, RAB10 silencing induces the intracellular retention of ABCB4 and then indirectly diminishes its secretory function. Taken together, our findings suggest that RAB10 regulates the plasma membrane targeting of ABCB4 and consequently its capacity to mediate phosphatidylcholine secretion.     

Recent progress in the manufacture of alkanesulfonates: Photosulfochlorination of single-chain length (C12−C16) n-alkanes using SO2Cl2 at high conversion rates in pure phase and in the presence of solvent

Increasing concentration of sulfuryl chloride during the photosulfochlorination reaction under visible light shows that under these conditions n-alkanes react at high conversion rates instead of the conversion rate of 15% reported in the literature. This photosulfochlorination with sulfuryl chloride leads to better and more interesting results compared with those of photosulfochlorination by gas mixture. Indeed, nearly total conversion of n-alkanes, specifically n-heptane, n-dodecane, n-tetradecane, and n-hexadecane, occurred in pure phase, with a quantitative global yield, a sulfochlorination-to-chlorination molar ratio of about 1, and a relative reactivity of secondary to primary hydrogen of about 2.5, at a reaction temperature of 30°C and a reaction time of 120 min, using 2×10⁻² mol L⁻¹ of pyridine. Under these conditions, no polysulfochlorinated compounds are detected. These results are further improved using chlorobenzene as the solvent, instead of benzene. Indeed, the sulfochlorination of n-heptane at a conversion rate of 80% in the presence of chlorobenzene leads also to a quantitative reaction yield, a higher RSO₂Cl/RCl molar ratio, and, as expected, a high selectivity of secondary over primary hydrogen. Under these conditions, sulfochlorination of long-chain n-alkanes leads to the highest RSO₂Cl/RCl molar ratio for n-dodecane, n-tetradecane, and n-hexadecane belonging to detergent range, and the value of the molar ratio for these is between 1.45 and 1.7. The isomeric distribution of sulfochlorinated compounds obtained during sulfochlorination in the presence of solvent resembles that of secondary alkanesul-fonates produced by sulfoxidation reaction, whereas that obtained in pure phase has a similar conversion rate, is rich in primary isomer, and thus is different from that of classic radical reactions such as photochlorination or photosulfochlorination with gas mixture.     

Design of processes of austenization and structure formation in surface hardening of forming rolls conducted with the use of power current inductors

An algorithm and software for analyzing and optimizing the processes of austenization and formation of martensitic and bainitic structural zones in the hardened layer due to continuous serial heating and hardening with the use of sectioned power current inductors are developed. The suggested computer technology based on criteria of the fullness of occurrence of processes of homogeneous austenization and on thermokinetic diagrams of austenitic transformation makes it possible to compute the heating power, the velocity of motion of the inductor, the number of passes in heating, the number of sections in the inductor, their sizes and position, and the width of the cooling zone required for ensuring the specified thickness and structure in the hardened layer. __________ Translated from Metallovedenie i Termicheskaya Obrabotka Metallov, No. 7, pp. 43–48, July, 2006.     

Polyesters bearing furan moieties

Summary This paper describes a preliminary set of results concerning novel systems in which the synthesis of furanic polyesters was conducted according to a transesterification procedure. Regular linear macromolecular structures were thus obtained with molecular weights well above those previously reported for similar polyesters prepared by more conventional routes.     

Multi-criteria decision making in workforce choice using AHP,WSM and WPM

This paper focuses on the implementation of Multi-Criteria Decision Making (MCDM) methods to evaluate the workforce competence in apparel industry. In this study, the Analytic Hierarchy Process (AHP), the Weighted Sum Model (WSM) and the Weighted Product Model (WPM) are suggested to solve workforce selection problem. The use of these methods is expected to help choose the best operator among several alternatives. Based on three criteria, each worker is evaluated in each executed operation. The criteria are a Quality Index ‘QI’, an Activity ‘A’ and an Attendance Rate ‘AR’. Thanks to the MCDM methods, scores allowing an objective classification of operators are obtained. Experimental results on AHP, WSM and WPM methods show the same decisions with the same workers’ classification from the best to the worst alternative. The resulting database contributes to the line balancing optimization by choosing for each operation, and as far as possible, the most competent operator.     

Combined passivity based control and optimal control for energy management of fuel cell/battery hybrid system

The energy management of hybrid electric vehicles is becoming an interesting topic for many researchers. Furthermore, the wise choice of the energy management strategy allows not only the best distribution of the power between the used sources, but also it allows reduction of consumption, increase in the lifetime of the sources, and improves the autonomy of the hybrid electric vehicle. The autonomy is guaranteed by the optimization of the embedded sources. In this study, the hybrid system consists of combining the fuel cell as the main source with the battery as the auxiliary source. The novelty of the proposed energy management strategy for the studied hybrid system is the combination between interconnection and damping assignment‐passivity based control and the Hamiltonian Jacobi Bellman method. The stability proof is given and the efficiency of the proposed strategy is proved by the experimental work, where the obtained results show the good and adequate results to the proposed scenario.     

Gründerzeitliche Mauerwerksbauten unter Erdbebeneinwirkung – Tragverhalten im Widerspruch zur aktuell angewandten Nachbemessung

Historic masonry buildings under earthquakes – Load‐bearing behaviour in contradiction to the currently applied methods of analysis The stability of historic masonry buildings must be guaranteed not only under normal conditions, but also during natural disasters. The seismic assessment of the masonry buildings of the Gründerzeit (1840–1918) in Vienna is a central topic in the qualitative and constructive assessment. Although masonry construction has been used for many centuries, the realistic evaluation of the load‐bearing behaviour is still a complex challenge. The methods of analysis according to current regulations are only insufficiently able to reflect the real load‐bearing behaviour and the possible activation of global failure mechanisms. As a result, the simplified verification is often difficult to calculate for many historic buildings, and questionable reinforcement measures are taken to compensate, even though the buildings have already experienced several earthquakes and survived most of them without damage. The present work deals with the approaches of current methods of analysis and aims to identify problem points and to compare them with time history analysis, which is supported by a powerful material model based on test series. It is shown that the conventional analysis for the historic masonry buildings without consideration of the interaction and load transfer effects as well as the characteristic construction methods only partially reflect the real load‐bearing behaviour. The work is intended to be a contribution to the technical expert discussions on the seismic safety of historic buildings and to stimulate the discussion on the formulation of realistic methods of analysis.     

Transmission and Reflection of Water-Wave on a Floating Ship in Vast Oceans

In this paper, we study the water-wave flow under a floating body of an incident wave in a fluid. This model simulates the phenomenon of waves abording a floating ship in a vast ocean. The same model, also simulates the phenomenon of fluid-structure interaction of a large ice sheet in waves. According to this method. We divide the region of the problem into three subregions. Solutions, satisfying the equation in the fluid mass and a part of the boundary conditions in each subregion, are given. We obtain such solutions as infinite series including unknown coefficients. We consider a limited number only of the coefficients by truncating the infinite series and satisfy the remaining boundary conditions approximately. Numerical experiments show that the results are acceptable. Tables are given along with the graph of the system of the resulting streamlines and the dynamical pressure acting on the obstacle. The drawn system of streamlines shows the correctness of the solution and the pressure is maximum on the side facing the upstream extremity of the channel. The same procedure can be adequately applied for problems with more complicated geometry and other phenomenon can thus be simulated.     

Thermal behavior of fat droplets as related to adsorbed milk proteins in complex food emulsions. A DSC study

The thermal behavior of hydrogenated palm kernel oil-in-water emulsions, which differed in their milk-protein composition, was studied in parallel with other characteristic parameters such as the aggregation/coalescence of fat droplets, and the proportion of adsorbed proteins at the oil/water interface. DSC was applied to monitor the crystallization and melting behavior of nonemulsified and emulsified fat samples. Comparison between nonemulsified and emulsified fat samples showed that in emulsified samples the initial temperature of fat crystallization and the temperature of the completion of melting were invariably lower and slightly higher, respectively. Furthermore, in complex food emulsions the supercooling temperature needed to initiate fat crystallization and the variation in its growth rate in the cooling experiment were dependent on the amount and nature of the adsorbed proteins. Our results indicate that the total replacement of milk proteins by whey proteins affected the fat crystallization behavior of emulsified fat droplets, in parallel with changes in their protein surface coverage and in their physical stability against fat droplet agglomeration.