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An ideal, hydrated, nondilute pseudobinary salt-protein-water solution model of the RBC intracellular solution has been developed to describe the osmotic behavior of human erythrocytes during freezing and thawing. Because of the hydration of intracellular solutes (mostly cell proteins), our analytical results predict that at least 16.65% of the isotonic cell water content will be retained within RBCs placed in hypertonic solutions. These findings are consistent not only with the experimental measurements of the amount of isotonic cell water retained within RBCs subjected to nonisotonic extracellular solutions (20-32%) but also with the experimental evidence that all of the water within RBCs is solvent water. By modeling the RBC intracellular solution as a hydrated salt-protein-water solution, no anomalous osmotic behavior is apparent. 相似文献
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Nucleophilic displacement of chlorine from polyvinylchloride (PVC) suspended either in water or in solution can be achieved using a nucleophile such as a sodium thiolate (R? S?, Na+). The nucleophilicity of the salt increases if an ether linkage exists in a β position to the thiol group [R? O? (CH2)2? S?, Na+]. Addition of a solvent such as cyclohexanone (which is a good solvent for PVC) to the slurry increases substantially the degree of substitution. Elemental analysis shows that every chlorine displaced from the polymer is replaced by a thiolate group; thus almost no dehydrochlorination occurs. It is possible to obtain by this method a grafted polymer in which 33% of the chlorine atoms are replaced by the β-ether thiolate group. The resulting polymer behaves like an internally plasticized PVC. The higher the degree of substitution of chlorine, the greater the flexibility of the grafted polymer is. Other compounds, such as lauryl thiolate and diethyl dithiocarbamate, also were used as nucleophiles, the latter one resulting in a brittle crosslinked polymer whenever sulfur was above 2%. Grafting of polytetrahydrofuran from PVC also was achieved using tetrahydrofuran as solvent and silver-perchlorate as catalyst. The resulting flexible grafted polymer is believed to consist of longer chains of polytetrahydrofuran grafted from few displaced chlorine atoms along the PVC chain. 相似文献
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