Identification of tubulin drug binding sites and prediction of relative differences in binding affinities to tubulin isotypes using digital signal processing

Microtubules are involved in numerous cellular processes including chromosome segregation during mitosis and, as a result, their constituent protein, tubulin, has become a successful target of several chemotherapeutic drugs. In general, these drugs bind indiscriminately to tubulin within both cancerous and healthy cells, resulting in unwanted side effects. However, differences between beta-tubulin isotypes expressed in a wide range of cell types may aid in the development of anti-tubulin drugs having increased specificity for only certain types of cells. Here, we describe a digital signal processing (DSP) method that is capable of predicting hot spots for the tubulin family of proteins as well as determining relative differences in binding affinities to these hot spots based only on the primary sequence of 10 human tubulin isotypes. Due to the fact that several drug binding sites have already been characterized within beta-tubulin, we are able to correlate hot spots with the binding sites for known chemotherapy drugs. We have also verified the accuracy of this method using the correlation between the binding affinities of characterized drugs and the tubulin isotypes. Additionally, the DSP method enables the rapid estimation of relative differences in binding affinities within the binding sites of tubulin isotypes that are yet to be experimentally determined.     

Utilizing Wiki-Systems in higher education classes: a chance for universal access?

Wikis are a website technology for mass collaborative authoring. Today, wikis are increasingly used for educational purposes. Basically, the most important asset of wikis is free and easy access for end users: everybody can contribute, comment and edit—following the principles of Universal access. Consequently, wikis are ideally suited for collaborative learning and a number of studies reported a great success of wikis in terms of active participation, collaboration, and a rapidly growing content. However, the wikis success in education was often linked either to direct incentives or even pressure. This paper strongly argues that this contradicts the original intentions of wikis and, furthermore, weakens the psycho-pedagogical impact. A study is presented which focuses on investigating the success of wikis in higher education, when students are neither enforced to contribute nor directly rewarded similar to the principles of Wikipedia. Amazingly, the results show that, in total, none of the N = 287 students created new articles or edited existing ones during a whole semester. It is concluded that the use of Wiki-Systems in educational settings is much more complicated, and it needs more time to develop a kind of “give-and-take” generation.     

Optimization strategy in the development process with application to cast components

At the development of commercial vehicles, it is necessary to develop cost-optimized components with respect to their entire lifetime. This requires a product development process that utilizes the possibility to simulate the product performance using modeling of different degrees of sophistication at any predefined level-of-detail (LOD). For example, the mechanical properties of a cast component depend strongly on the casting process, the simulation of which requires complex modeling at a high LOD. In this article, we propose an optimization strategy that is based on the LOD concept to develop cost-effective components. A sizing optimization of a cast model component is carried out with respect to both the mechanical and thermal response to exemplify the process. In particular, a (novel) global measure of the temperature in energy norm is introduced as an indicator of the “production fitness” to ensure high quality of the material properties. This measure is the thermal counterpart of the well-accepted mechanical strain energy.     

Low retaining force optical viewport seal

We report on the experimental realization of an ultrahigh vacuum (UHV) indium sealing between a conflat knife edge and an optical window. The sealing requires a very low clamping force and thus allows for the use of very thin and fragile windows.     

Light scattering from filaments

Photorealistic visualization of a huge number of individual filaments like in the case of hair, fur, or knitwear is a challenging task: Explicit rendering approaches for simulating radiance transfer at a filament get totally impracticable with respect to rendering performance and it is also not obvious how to derive efficient scattering functions for different levels of (geometric) abstraction or how to deal with very complex scattering mechanisms. We present a novel uniform formalism for light scattering from filaments in terms of radiance, which we call the bidirectional fiber scattering distribution function (BFSDF). We show that previous specialized approaches, which have been developed in the context of hair rendering, can be seen as instances of the BFSDF. Similar to the role of the BSSRDF for surface scattering functions, the BFSDF can be seen as a general approach for light scattering from filaments, which is suitable for deriving approximations in a canonic and systematic way. For the frequent cases of distant light sources and observers, we deduce an efficient far field approximation (bidirectional curve scattering distribution function, BCSDF). We show that on the basis of the BFSDF, parameters for common rendering techniques can be estimated in a non-ad-hoc, but physically-based way     

Segmentation of microscope images of living cells

This paper describes a segmentation method combining a texture based technique with a contour based method. The technique is designed to enable the study of cell behaviour over time by segmenting brightfield microscope image sequences. The technique was tested on artificial images, based on images of living cells and on real sequences acquired from microscope observations of neutrophils and lymphocytes as well as on a sequence of MRI images. The results of the segmentation are compared with the results of the watershed and snake segmentation methods. The results show that the method is both effective and practical.

B2C-E-Commerce als Treiber simultaner Veränderungen in IT-Struktur und Markenarchitektur

We analyze the influence of B2C e-commerce on IT structure and brand architecture based on conceptual considerations, a case study and an empirical survey. We first discuss the changes in transaction costs that are induced by B2C e-commerce. The effects of B2C e-commerce on IT structure and brand architecture, which are anticipated from a transaction cost perspective, are illustrated using a case study from the tourism industry. We conducted a quantitative study among 101 chief information officers (CIOs) and chief marketing officers (CMOs) from the most important consumer brand companies in Austria. The results of a correspondence analysis show that a high importance of e-commerce triggers a typical bundle of changes in IT structure and brand architecture, which point toward a stronger integration within and between these two structural elements. We therefore conclude that B2C e-commerce calls for a better coordination of those measures that impact IT structure and brand architecture.