全文获取类型
收费全文 | 349篇 |
免费 | 30篇 |
专业分类
化学工业 | 141篇 |
金属工艺 | 4篇 |
机械仪表 | 7篇 |
建筑科学 | 7篇 |
能源动力 | 12篇 |
轻工业 | 87篇 |
水利工程 | 2篇 |
石油天然气 | 5篇 |
无线电 | 19篇 |
一般工业技术 | 38篇 |
冶金工业 | 3篇 |
原子能技术 | 5篇 |
自动化技术 | 49篇 |
出版年
2023年 | 6篇 |
2022年 | 33篇 |
2021年 | 39篇 |
2020年 | 17篇 |
2019年 | 14篇 |
2018年 | 14篇 |
2017年 | 10篇 |
2016年 | 14篇 |
2015年 | 17篇 |
2014年 | 23篇 |
2013年 | 17篇 |
2012年 | 29篇 |
2011年 | 34篇 |
2010年 | 12篇 |
2009年 | 30篇 |
2008年 | 15篇 |
2007年 | 21篇 |
2006年 | 10篇 |
2005年 | 7篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2002年 | 2篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1990年 | 1篇 |
1984年 | 1篇 |
排序方式: 共有379条查询结果,搜索用时 15 毫秒
11.
In this paper, we consider the problem of automatic verification of SMIL documents and present a tool which can assist the
user in the complex task of authoring a multimedia presentation. The tool is based on a formal semantics defining the temporal
aspects of SMIL elements by means of a set of inference rules. The rules, in the spirit of Hoare’s semantics, describe how
the execution of a piece of code changes the state of the computation of a player. If any temporal conflict is found, the
system returns a message to the user pointing out the element which contains the conflict and its motivation. This helps the
user to develop robust and clear code. 相似文献
12.
Relevance feedback has recently emerged as a solution to the problem of improving the retrieval performance of an image retrieval
system based on low-level information such as color, texture and shape features. Most of the relevance feedback approaches
limit the utilization of the user’s feedback to a single search session, performing a short-term learning. In this paper we
present a novel approach for short and long term learning, based on the definition of an adaptive similarity metric and of
a high level representation of the images. For short-term learning, the relevant and non-relevant information given by the
user during the feedback process is employed to create a positive and a negative subspace of the feature space. For long-term
learning, the feedback history of all the users is exploited to create and update a representation of the images which is
adopted for improving retrieval performance and progressively reducing the semantic gap between low-level features and high-level
semantic concepts. The experimental results prove that the proposed method outperforms many other state of art methods in
the short-term learning, and demonstrate the efficacy of the representation adopted for the long-term learning.
相似文献
Annalisa FrancoEmail: |
13.
Maria Addolorata Bonifacio Riccardo Laterza Angela Vinella Annalisa Schirinzi Mariangela Defilippis Francesca Di Serio Angelo Ostuni Antonio Fasanella Maria Addolorata Mariggi 《International journal of molecular sciences》2022,23(17)
Serological assays are useful in investigating the development of humoral immunity against SARS-CoV-2 in the context of epidemiological studies focusing on the spread of protective immunity. The plaque reduction neutralization test (PRNT) is the gold standard method to assess the titer of protective antibodies in serum samples. However, to provide a result, the PRNT requires several days, skilled operators, and biosafety level 3 laboratories. Therefore, alternative methods are being assessed to establish a relationship between their outcomes and PRNT results. In this work, four different immunoassays (Roche Elecsys® Anti SARS-CoV-2 S, Snibe MAGLUMI® SARS-CoV-2 S-RBD IgG, Snibe MAGLUMI® 2019-nCoV IgG, and EUROIMMUN® SARS-CoV-2 NeutraLISA assays, respectively) have been performed on individuals healed after SARS-CoV-2 infection. The correlation between each assay and the reference method has been explored through linear regression modeling, as well as through the calculation of Pearson’s and Spearman’s coefficients. Furthermore, the ability of serological tests to discriminate samples with high titers of neutralizing antibodies (>160) has been assessed by ROC curve analyses, Cohen’s Kappa coefficient, and positive predictive agreement. The EUROIMMUN® NeutraLISA assay displayed the best correlation with PRNT results (Pearson and Spearman coefficients equal to 0.660 and 0.784, respectively), as well as the ROC curve with the highest accuracy, sensitivity, and specificity (0.857, 0.889, and 0.829, respectively). 相似文献
14.
Katia Grillone Caterina Riillo Roberta Rocca Serena Ascrizzi Virginia Span Francesca Scionti Nicoletta Poler Annalisa Maruca Marilia Barreca Giada Juli Mariamena Arbitrio Maria Teresa Di Martino Daniele Caracciolo Pierosandro Tagliaferri Stefano Alcaro Alessandra Montalbano Paola Barraja Pierfrancesco Tassone 《International journal of molecular sciences》2022,23(18)
Microtubule-targeting agents (MTAs) are effective drugs for cancer treatment. A novel diaryl [1,2]oxazole class of compounds binding the colchicine site was synthesized as cis-restricted-combretastatin-A-4-analogue and then chemically modified to have improved solubility and a wider therapeutic index as compared to vinca alkaloids and taxanes. On these bases, a new class of tricyclic compounds, containing the [1,2]oxazole ring and an isoindole moiety, has been synthetized, among which SIX2G emerged as improved MTA. Several findings highlighted the ability of some chemotherapeutics to induce immunogenic cell death (ICD), which is defined by the cell surface translocation of Calreticulin (CALR) via dissociation of the PP1/GADD34 complex. In this regard, we computationally predicted the ability of SIX2G to induce CALR exposure by interacting with the PP1 RVxF domain. We then assessed both the potential cytotoxic and immunogenic activity of SIX2G on in vitro models of multiple myeloma (MM), which is an incurable hematological malignancy characterized by an immunosuppressive milieu. We found that the treatment with SIX2G inhibited cell viability by inducing G2/M phase cell cycle arrest and apoptosis. Moreover, we observed the increase of hallmarks of ICD such as CALR exposure, ATP release and phospho-eIF2α protein level. Through co-culture experiments with immune cells, we demonstrated the increase of (i) CD86 maturation marker on dendritic cells, (ii) CD69 activation marker on cytotoxic T cells, and (iii) phagocytosis of tumor cells following treatment with SIX2G, confirming the onset of an immunogenic cascade. In conclusion, our findings provide a framework for further development of SIX2G as a new potential anti-MM agent. 相似文献
15.
Giuditta Guerrini Sabrina Gioria Aisha V. Sauer Simone Lucchesi Francesca Montagnani Gabiria Pastore Annalisa Ciabattini Donata Medaglini Luigi Calzolai 《International journal of molecular sciences》2022,23(16)
PEGylated lipids are one of the four constituents of lipid nanoparticle mRNA COVID-19 vaccines. Therefore, various concerns have been raised on the generation of anti-PEG antibodies and their potential role in inducing hypersensitivity reactions following vaccination or in reducing vaccine efficacy due to anti-carrier immunity. Here, we assess the prevalence of anti-PEG antibodies, in a cohort of vaccinated individuals, and give an overview of their time evolution after repeated vaccine administrations. Results indicate that, in our cohort, the presence of PEG in the formulation did not influence the level of anti-Spike antibodies generated upon vaccination and was not related to any reported, serious adverse effects. The time-course analysis of anti-PEG IgG showed no significant booster effect after each dose, whereas for IgM a significant increase in antibody levels was detected after the first and third dose. Data suggest that the presence of PEG in the formulation does not affect safety or efficacy of lipid-nanoparticle-based COVID-19 vaccines. 相似文献
16.
Annalisa Paternò Daniela Verginelli Pamela Bonini Marisa Misto Cinzia Quarchioni Enrico Dainese Stefania Peddis Cristiana Fusco Daniela Vinciguerra Ugo Marchesi 《Food Analytical Methods》2018,11(5):1281-1290
Common wheat is one of the most important staple food crops worldwide. However, unlike other important staple crops such as maize or soybean, genetically modified (GM) wheat is not yet present in the global food market. Nonetheless, in the recent past, the adventitious presence of GM glyphosate-tolerant volunteers was reported in open wheat fields in the USA. The European Union Reference Laboratory for GM Food and Feed (EURL-GMFF) was therefore called to develop a strategy to detect such unauthorised GM wheat in wheat samples by using both taxon-specific and screening tests. Two candidate common wheat taxon-specific real-time PCR methods were suggested, one targeting ssII-D gene coding for starch synthase and the other targeting waxy-D1 gene, coding for granule-bound starch synthase. In the present study, the two above-mentioned real-time PCR taxon-specific methods were in-house verified and compared, proposing droplet digital PCR (ddPCR) as a new tool for supporting the application of the European Network of GMO Laboratories (ENGL) established method performance criteria. Preliminary performance data of waxy-D1 and ssII-D methods in ddPCR format are shown too to give a contribution to the bridging process from the consolidated to the emerging quantitative PCR methodology. 相似文献
17.
Influence of olive ripening degree and crusher typology on chemical and sensory characteristics of Correggiolo virgin olive oil
下载免费PDF全文
![点击此处可从《Journal of the science of food and agriculture》网站下载免费的PDF全文](/ch/ext_images/free.gif)
18.
Baffone W Casaroli A Citterio B Pierfelici L Campana R Vittoria E Guaglianone E Donelli G 《International journal of food microbiology》2006,107(1):83-91
Water is known as one of the main transmission routes of Campylobacter and contributes to increase the number of sporadic infections and outbreaks. Campylobacter jejuni persists in the environment, especially in water, in a viable but non-culturable (VBNC) form that is thought to be a possible cause of water-borne outbreaks. In this study, we evaluated the loss of culturability and viability of 9 C. jejuni strains of clinical origin and one ATCC reference strain when kept at 4 degrees C in artificial sea water (ASW). Culturability was measured as colony-forming units while viability was evaluated by CTC-DAPI double staining and the combined CTC-specific fluorescent antibody technique (CTC-FA). When cultured on Columbia Agar plates, strains exhibited different growth profiles which allowed to classify them into three different groups. Both techniques used to monitor the viability of the bacterial cells showed that C. jejuni strains survived in the VBNC form in the microcosms through a period lasting from 138 to 152 days. The recovery of C. jejuni VBNC forms to culturability, as evidenced by cell division, was obtained by passage in the mouse intestine. Our results indicate that C. jejuni VBNC cells were able to remain in this state for a few months and regain their culturability after in vivo passage depending on their lasting in the VBNC state, which affects the number of respiring bacteria. In fact, the resuscitation was achieved when the number of respiring bacteria became higher than 10(4) cell/ml. Therefore, a relatively high microbial titer of respiring bacteria in the VBNC state seems to be important for the resuscitation and subsequent intestinal colonisation. 相似文献
19.
20.
Annalisa Bernareggi Alessandra Bosutti Gabriele Massaria Rashid Giniatullin Tarja Malm Marina Sciancalepore Paola Lorenzon 《International journal of molecular sciences》2022,23(12)
Piezo1 channels are highly mechanically-activated cation channels that can sense and transduce the mechanical stimuli into physiological signals in different tissues including skeletal muscle. In this focused review, we summarize the emerging evidence of Piezo1 channel-mediated effects in the physiology of skeletal muscle, with a particular focus on the role of Piezo1 in controlling myogenic precursor activity and skeletal muscle regeneration and vascularization. The disclosed effects reported by pharmacological activation of Piezo1 channels with the selective agonist Yoda1 indicate a potential impact of Piezo1 channel activity in skeletal muscle regeneration, which is disrupted in various muscular pathological states. All findings reported so far agree with the idea that Piezo1 channels represent a novel, powerful molecular target to develop new therapeutic strategies for preventing or ameliorating skeletal muscle disorders characterized by an impairment of tissue regenerative potential. 相似文献