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101.
102.
Antonella De Leonardis Vincenzo Macciola Giuseppe Lustrato Giancarlo Ranalli Ahindra Nag 《European Food Research and Technology》2010,230(3):405-410
In the last few decades, biotechnological applications of phenoloxidase enzymes have become an area of significant interest.
In this study, sunflower seeds, seedlings and defatted mill cake were investigated as possible plant source of polyphenoloxidase
(PPO). Noticeable variation of chlorogenic acid concentration in each raw material has undoubtedly proven that only sunflower
seedlings have significant amounts of active PPO. The activity of the enzymes was assessed by measuring the molar decrease
of caffeic acid. Isolated protein powders from each raw material confirmed the presence of PPO only in the seedlings. Catalytic
action of the PPO of seedlings was compared to that of the commercial laccase from Trametes versicolor. Sunflower PPO was selectively active on caffeic and chlorogenic acids, less active on ferulic acid and not active on mono-phenols
and gallic acid. Conversely, laccase was highly active on all the assessed phenols. PPO activity was good in a large range
of pH (4–8), whilst it was approximately halved in solutions containing 35% ethyl alcohol (v/v), 500 mg L−1 citric acid and finally 200 mg L−1 sulphur dioxide. In conclusion, sunflower seedlings can be considered a potential and interesting plant source of PPO. Sunflower
PPO could be used to oxidise selectively o-diphenols, for example in alcoholic and nonalcoholic beverages. 相似文献
103.
Eleonora Polo Fabrizio Forlini Valerio Bertolasi Antonella Caterina Boccia Maria Carmela Sacchi 《Advanced Synthesis \u0026amp; Catalysis》2008,350(10):1544-1556
We report here the synthesis of new tethered biscyclopentadienyl and bisindenyl zirconocenes, bearing one unsaturation on the interannular bridge, and their use as self‐immobilizing catalysts. They proved to be active catalysts towards ethylene polymerization in solution, with activities comparable to those displayed by commercial rac‐Et(Ind)2ZrCl2. When tested as self‐polymerization catalysts under suitable experimental conditions, they gave colored precipitates that, once reactivated with MAO, were significantly active in ethylene polymerization, although lower than those of the corresponding catalytic systems in solution. The molecular weights of the produced polymers were similar to those obtained with the same catalysts in solution, but their distribution resulted to be broader, with values typical of heterogeneous catalytic systems. From 13C NMR studies we had the first spectroscopic evidence of the actual incorporation of a metallocene of this type into a polymeric chain. 相似文献
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107.
Antonella Gori Cecilia Brunetti Luana Beatriz dos Santos Nascimento Giovanni Marino Lucia Guidi Francesco Ferrini Mauro Centritto Alessio Fini Massimiliano Tattini 《International journal of molecular sciences》2021,22(15)
Carotenoids and phenylpropanoids play a dual role of limiting and countering photooxidative stress. We hypothesize that their “antioxidant” function is prominent in plants exposed to summer drought, when climatic conditions exacerbate the light stress. To test this, we conducted a field study on Phillyrea latifolia, a Mediterranean evergreen shrub, carrying out daily physiological and biochemical analyses in spring and summer. We also investigated the functional role of the major phenylpropanoids in different leaf tissues. Summer leaves underwent the most severe drought stress concomitantly with a reduction in radiation use efficiency upon being exposed to intense photooxidative stress, particularly during the central hours of the day. In parallel, a significant daily variation in both carotenoids and phenylpropanoids was observed. Our data suggest that the morning-to-midday increase in zeaxanthin derived from the hydroxylation of ß-carotene to sustain non-photochemical quenching and limit lipid peroxidation in thylakoid membranes. We observed substantial spring-to-summer and morning-to-midday increases in quercetin and luteolin derivatives, mostly in the leaf mesophyll. These findings highlight their importance as antioxidants, countering the drought-induced photooxidative stress. We concluded that seasonal and daily changes in photosynthetic and non-photosynthetic pigments may allow P. latifolia leaves to avoid irreversible photodamage and to cope successfully with the Mediterranean harsh climate. 相似文献
108.
This paper presents the design of a new adaptive optimization‐based second‐order sliding mode control algorithm for uncertain nonlinear systems. It is designed on the basis of a second‐order sliding mode control with optimal reaching, with the aim of reducing the control effort while maintaining all the positive aspects in terms of finite‐time convergence and robustness in front of matched uncertainties. These features are beneficial to guarantee good performance in case of vehicle dynamics control, a crucial topic in the light of the increasing demand of semiautonomous and autonomous driving capabilities in commercial vehicles. The new proposal is theoretically analyzed, as well as verified relying on an extensive comparative study, carried out on a realistic simulator of a 4‐wheeled vehicle, in the case of a lateral stability control system. 相似文献
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Maria Carmela Annosi Alberto Monti Antonella Martini 《Creativity & Innovation Management》2020,29(3):528-545
Previous research has shown the importance of individual learning goal orientation for both job and task performance and consequently organizational performance. Despite its importance, knowledge on the antecedents of learning goal orientation remains scarce, especially in the context of self‐managing team‐based organizations. In fact, most of the research on goal orientation antecedents has been focused on individual characteristics, belief, and ability, while the contextual factors that might influence them remain unspecified. We build on and further extend earlier studies by jointly exploring the role of individual and contextual factors affecting individual learning orientation. In particular, this study combines individual informal social network, self‐efficacy, performance feedbacks, and team identification into a model that explains individuals' learning goal orientation within self‐managing team‐based organizations. The model was empirically tested on a sample of 104 individuals belonging to an R&D organization relying on self‐managing teams. Results show that performance feedback has a negative direct effect, while team identification has a positive direct effect on individual learning goal orientation. In addition, we found that individual self‐efficacy is a mediator of the relationships between performance feedback and brokerage in the advice network and individual learning goal orientation. Finally, we did not find a relationship between centrality in the friendship network and individual learning goal orientation. 相似文献