Electrochemical treatment of olive mill wastewater

Background Olive mill wastewater (OMW) constitutes a very strong agro‐industrial wastewater posing severe environmental threats in olive oil producing countries. The main objective of this study was to treat olive mill wastewater by electrochemical oxidation. The variables studied included the type and concentration of electrolyte solutions, voltage and time applied. Results: The electrolyte type and concentration significantly affected the degradation efficiency of the electrochemical oxidation. Optimal conditions for NaCl concentration were 3% (w/v) and 16 V. At these conditions chemical oxygen demand (COD) removal reached 70.8% after 8 h of electrochemical treatment, while color and turbidity were completely removed after short periods of treatment. However, bio‐assays indicated that the ecotoxicity of the treated wastewater remained unchanged, possibly due to the formation of chlorinated by‐products. Na₂SO₄ did not demonstrate sufficient efficiency. The simultaneous use of FeCl₃ and NaCl contributed to electro‐coagulation of OMW. After settlement, two separate phases were formed: the supernatant phase and the settled solids. Under optimal conditions (2% Na₂SO₄ + 1% FeCl₃; 24 V), the removal efficiency of COD reached 85.5% at the supernatant phase. Conclusion: NaCl was an effective electrolyte for OMW treatment. The electro‐coagulation process was also a successful process, but as in the case of NaCl the remaining acute toxicity of treated OMW was high. Copyright © 2007 Society of Chemical Industry     

Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece

The examination of 2005 raw bovine (n = 950), caprine (n = 460) and ovine (n = 595) bulk milk samples collected throughout several regions in Greece for the presence of Escherichia coli serogroup O157 resulted in the isolation of 29 strains (1.4%) of which 21 were isolated from bovine (2.2%), 3 from caprine (0.7%) and 5 from ovine (0.8%) milk. Out of the 29 E. coli O157 isolates, only 12 (41.4%) could be classified as Shiga-toxigenic based on immunoassay and PCR results. All 12 Shiga-toxigenic E. coli serogroup O157 isolates belonged to the E. coli O157:H7 serotype. All except one of the 12 Shiga-toxin positive isolates were stx₂-positive, five of which were also stx₁-positive. The remaining isolate was positive only for the stx₁ gene. All stx-positive isolates (whether positive for stx₁, stx₂ or stx₁ and stx₂) were also PCR-positive for the eae and ehxA genes. The remaining 17 E. coli O157 isolates (58.6%) were negative for the presence of the H7 flagellar gene by PCR, tested negative for Shiga-toxin production both by immunoassay and PCR, and among these, only four and three strains were PCR-positive for the eae and ehxA genes, respectively. All 29 E. coli O157 isolates displayed resistance to a wide range of antimicrobials, with the stx-positive isolates being, on average, resistant to a higher number of antibiotics than those which were stx-negative.     

Growth of Aeromonas hydrophila in the whey cheeses Myzithra, Anthotyros, and Manouri during storage at 4 and 12 degrees C

The fresh whey cheeses Myzithra, Anthotyros, and Manouri were inoculated with Aeromonas hydrophila strain NTCC 8049 (type strain) or with an A. hydrophila strain isolated from food (food isolate) at levels of 3.0 to 5.0 x 10(2) CFU/g of cheese and stored at 4 or 12 degrees C. Duplicate samples of cheeses were tested for levels of A. hydrophila and pH after up to 29 days of storage. At 4 degrees C, A. hydrophila grew in Myzithra and Anthotyros with a generation time of ca. 19 h, but no growth was observed in Manouri. In Myzithra, average maximum populations of 8.87 log CFU/g (type strain) and 8.79 log CFU/g (food isolate) were recorded after 20 and 22 days of storage at 4 degrees C, respectively. The average maximum populations observed in Anthotyros stored at 4 degrees C were 6.72 log CFU/g (food isolate) and 6.13 log CFU/g (type strain) and were observed after 15 and 16 days of storage, respectively. A. hydrophila grew rapidly and reached high numbers in cheeses stored at 12 degrees C. The average generation times were 3.7 and 3.9 h (Myzithra), 4.1 and 6.1 h (Anthotyros), and 8.0 and 9.2 h (Manouri) for the type strain and the food isolate, respectively. Among the different whey cheese trials, the highest A. hydrophila population recorded (10.13 log CFU/g) was in Myzithra that had been inoculated with the food isolate after 8 days of storage at 12 degrees C. To prevent A. hydrophila growth in whey cheeses, efforts must be focused on preventing postprocessing contamination and temperature abuse during transportation and storage.     

Study of aging in oil paintings by 1D and 2D NMR spectroscopy

Nuclear magnetic resonance spectroscopy is proposed as an efficient analytical tool in the study of painted artworks. The binding medium from two original oil paintings, dated from the early 20th and the late 17th century, was studied via high-resolution 1D and 2D NMR, establishing the advanced state of hydrolysis and oxidation of the oil paint. Studies of the solvent-extractable component from model samples of various drying oils, raw oil paints, and aged oil paints allowed the definition of several markers based on the integral ratios of various chemical species present in the 1H and 13C NMR spectra. These markers are sensitive to hydrolytic and oxidative processes that reflect the extent of aging in oil paintings. The rapidity, simplicity, and nondestructive nature of the proposed analytical NMR methodology represents a great advantage, since the usually minute sample quantities available from original artwork can be subsequently analyzed further by other analytical techniques, if necessary.     

Distributed resistance modeling of wire-wrapped rod bundles

Three-dimensional flow hydrodynamic distributed resistance models for rod bundles were developed. The models specifically account for the presence of the wire-wrap spacer and may be used for any lumped parameter thermohydraulic analysis numerical program. Validation studies of the hydrodynamic resistance models were also performed using a subchannel code ASFRE. The models were tested against subchannel velocity and temperature data taken from bundles of triangular rod array configurations with wire-spacers. Overall the models performed satisfactorily predicting the most important qualitative trends for flows in wire-wrapped rod bundles.     

Polysilicon TFT technology on flexible metal foil for AMPLED displays

Abstract— A top‐emitting 230‐dpi active‐matrix polymer light‐emitting diode (AMPLED) display, having a VGA format and a 3.3‐in.‐diagonal size, on a flexible stainless‐steel‐foil substrate is reported. The active‐matrix array was fabricated with laser‐crystallized polysilicon TFTs at a maximum process temperature of 700°C. The top‐emitting PLED diodes were prepared by spin‐casting organic light‐emitting polymers. This work demonstrates the compatibility of polysilicon‐TFT technology with flexible metal‐foil substrates for active‐matrix organic light‐emitting‐diode (AMOLED) display applications.     

Dynamic flow behavior measurements in gas–solid fluidized beds using different non-intrusive techniques and polyethylene powder

Pressure fluctuations and X-ray computed tomography (CT) measurements were utilized to characterize the flow behavior of gas–solid fluidized beds using polyethylene particles in three Plexiglas columns with diameters of 10, 20, and 30 cm. Air was used as the gas phase. The gas–solid flow dynamic under ambient conditions was characterized from statistical analysis of pressure fluctuation data and CT images. The time-averaged voidage distribution, bubble-phase area fraction, bubble diameter and bubble number distribution varying with the bed heights were extracted from all the three columns. The bed scales had significant effect on the hydrodynamics. The scale-up effects on the gas–solid two-phase flow behavior were discussed.     

International evidence on aggregate short-run and long-run interfuel substitution

In this paper, we build on recent work by Serletis et al. (2010, in press) and report short- and long-run estimates of aggregate interfuel substitution for a number of OECD and non-OECD countries. In doing so, we use recent pooled intercountry data (since 1980), and state-of-the-art advances in microeconometrics, including duality theory and flexible functional forms. Also, motivated by the widespread practice in the empirical energy demand literature of ignoring theoretical regularity, we estimate our model subject to global curvature (but not monotonicity), using methods developed by Diewert and Wales (1987). We provide inference, and also a policy perspective, using parameter estimates that are consistent with the theoretical regularity conditions of neoclassical microeconomic theory.