Elevated amniotic fluid levels of leukemia inhibitory factor, interleukin 6, and interleukin 8 in intra-amniotic infection

OBJECTIVE: The study's objective was to determine and correlate amniotic fluid levels of leukemia inhibitory factor, interleukin 6, and interleukin 8 in patients with and without intra-amniotic infection. STUDY DESIGN: Amniocentesis was performed on 41 pregnant women with preterm contractions, labor, or premature rupture of membranes. Intra-amniotic infection was defined as the presence of a positive amniotic fluid culture result. Amniotic fluid tests for Gram stain, glucose, leukocyte counts, creatinine level, pH, and specific gravity were performed. Amniotic fluid levels of leukemia inhibitory factor, interleukin 6, and interleukin 8 were measured by an enzyme-linked immunoassay. Unlike in previous reports, cytokines were normalized by amniotic fluid creatinine levels. RESULTS: Fifteen patients had intra-amniotic infection and 26 did not. Amniotic fluid median levels of leukemia inhibitory factor, interleukin 6, and interleukin 8 were significantly higher in pregnant women with intra-amniotic infection than in those without intra-amniotic infection (leukemia inhibitory factor median 3912 pg/mg creatinine, range 0.0-199314, vs 56 pg/mg creatinine, range 0. 0-12148, P =.01; interleukin 6 median 2005 ng/mg creatinine, range 27-4071, vs 990 ng/mg creatinine, range 7.5-3409, P =.005; interleukin 8: median 4933 ng/mg creatinine, range 0.0-55058, vs 61 ng/mg creatinine, range 0.0-2399, P =.005). Amniotic fluid levels of leukemia inhibitory factor, interleukin 6, and interleukin 8 were positively correlated. CONCLUSIONS: The data indicate that leukemia inhibitory factor plays an important role in the pathogenesis of intra-amniotic infection. In addition, significant elevations of and correlations among amniotic fluid levels of leukemia inhibitory factor, interleukin 6, and interleukin 8 suggest that measurements of these cytokines in amniotic fluid may be of diagnostic and prognostic importance.     

A hierarchical distributed-shared memory parallel Branch&Bound application with PVM and OpenMP for multiprocessor clusters

Branch&Bound (B&B) is a technique widely used to solve combinatorial optimization problems in physics and engineering science. In this paper we show how the combined use of PVM and OpenMP libraries can be a promising approach to exploit the intrinsic parallel nature of this class of application and to obtain efficient code for hybrid computational architectures. We described how both the shared memory and the distributed memory programming models can be applied to implement the same algorithm for the inter-nodes and intra-node parallelization. Some experimental tests on a local area network (LAN) of workstations are finally discussed.     

A simulation model for localization of pervasive objects using heterogeneous wireless networks

Position represents a relevant attribute needed by many applications, whose contexts are characterized by pervasiveness of the objects/things in the considered scenarios. In order to infer positions of pervasive objects, which are neither equipped with any location-sensing technologies, nor are unable to locate themselves, it is needed to support them with an appropriate infrastructure, which allows to determine their position in a manner that is transparent to applications. In this work, we aim at extending the positioning service using an agent-based approach, in order to discover and localize different kinds of objects, exploiting cheap and embedded technologies. We describe the design and the implementation of a layered architecture, that supports the localization of devices, and simple pervasive and ubiquitous objects. A simulation tool has been develop to evaluate the proposed solution in different application scenarios of every-day life.     

Experimental Evaluation of Quality Parameters During Drying of Carrot Samples

The aim of this work was to evaluate the effect of operating conditions on the quality of dried foods, monitoring shrinkage, rehydration capacity, and color changes. Carrot samples of different shapes and dimensions were dried in a convective oven by air whose temperature and velocity were chosen in a range of physical significance. Two values of air velocity, i.e., 2.8 and 2.2 m/s, were considered; air temperature was chosen equal to 50 °C, 70 °C, and 85 °C. It was observed that more drastic drying conditions indeed improved the drying rate, but were also responsible for a decrease of food rehydration capacity. Food behavior was observed comparing some characteristic parameters as obtained upon fitting the experimental data by simple modeling equations. In particular, the drying curves were fitted by the Newton’s model, whereas, shrinkage was modeled by a linear relationship between volume variation and moisture content. Rehydration capacity was estimated by water regain percentage, which was evaluated after 5 h.     

Biased T-cell antigen receptor repertoire in Lyme arthritis

A common concern with many autoimmune diseases of unknown etiology is the extent to which tissue T-lymphocyte infiltrates, versus a nonspecific infiltrate, reflect a response to the causative agent. Lyme arthritis can histologically resemble rheumatoid synovitis, particularly the prominent infiltration by T lymphocytes. This has raised speculation about whether Lyme synovitis represents an ongoing response to the causative spirochete, Borrelia burgdorferi, or rather a self-perpetuating autoimmune reaction. In an effort to answer this question, the present study examined the repertoire of infiltrating T cells in synovial fluid from nine Lyme arthritis patients, before and after stimulation with B. burgdorferi. Using a highly sensitive and consistent quantitative PCR technique, a comparison of the T-cell antigen receptor (TCR) beta-chain variable (Vbeta) repertoires of the peripheral blood and synovial fluid showed a statistically significant increase in expression of Vbeta2 and Vbeta6 in the latter. This is remarkably similar to our previous findings in studies of rheumatoid arthritis and to other reports on psoriatic skin lesions. However, stimulation of synovial fluid T cells with B. burgdorferi provoked active proliferation but not a statistically significant increase in expression of any TCR Vbeta, including Vbeta2 and Vbeta6. Collectively, the findings suggest that the skewing of the TCR repertoire of fresh synovial fluid in Lyme arthritis may represent more a synovium-tropic or nonspecific inflammatory response, similar to that occurring in rheumatoid arthritis or psoriasis, rather than a specific Borrelia reaction.