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131.
Dr. Ying Wang Prof. Dr. Andrew Ewing 《Chembiochem : a European journal of chemical biology》2021,22(5):807-813
Exocytosis plays an essential role in the communication between cells in the nervous system. Understanding the regulation of neurotransmitter release during exocytosis and the amount of neurotransmitter content that is stored in vesicles is of importance, as it provides fundamental insights to understand how the brain works and how neurons elicit a certain behavior. In this minireview, we summarize recent progress in amperometric measurements for monitoring exocytosis in single cells and electrochemical cytometry measurements of vesicular neurotransmitter content in individual vesicles. Important steps have increased our understanding of the different mechanisms of exocytosis. Increasing evidence is firmly establishing that partial release is the primary mechanism of release in multiple cell types. 相似文献
132.
Liangtao Yang Liang-Yin Kuo Juan Miguel López del Amo Prasant Kumar Nayak Katherine A. Mazzio Sebastian Maletti Daria Mikhailova Lars Giebeler Payam Kaghazchi Teófilo Rojo Philipp Adelhelm 《Advanced functional materials》2021,31(38):2102939
A known strategy for improving the properties of layered oxide electrodes in sodium-ion batteries is the partial substitution of transition metals by Li. Herein, the role of Li as a defect and its impact on sodium storage in P2-Na0.67Mn0.6Ni0.2Li0.2O2 is discussed. In tandem with electrochemical studies, the electronic and atomic structure are studied using solid-state NMR, operando XRD, and density functional theory (DFT). For the as-synthesized material, Li is located in comparable amounts within the sodium and the transition metal oxide (TMO) layers. Desodiation leads to a redistribution of Li ions within the crystal lattice. During charging, Li ions from the Na layer first migrate to the TMO layer before reversing their course at low Na contents. There is little change in the lattice parameters during charging/discharging, indicating stabilization of the P2 structure. This leads to a solid-solution type storage mechanism (sloping voltage profile) and hence excellent cycle life with a capacity of 110 mAh g-1 after 100 cycles. In contrast, the Li-free compositions Na0.67Mn0.6Ni0.4O2 and Na0.67Mn0.8Ni0.2O2 show phase transitions and a stair-case voltage profile. The capacity is found to originate from mainly Ni3+/Ni4+ and O2-/O2-δ redox processes by DFT, although a small contribution from Mn4+/Mn5+ to the capacity cannot be excluded. 相似文献
133.
Romero-Garcés A. Salles De Freitas R. Marfil R. Vicente-Chicote C. Martínez J. Inglés-Romero J. F. Bandera A. 《Multimedia Tools and Applications》2022,81(3):3603-3628
Multimedia Tools and Applications - The design of robots capable of operating autonomously in changing and unstructured environments, requires using complex software architectures in which,... 相似文献
134.
Belov M. I. Anashin D. V. Kabdin N. E. Storchevoy V. F. Sudnik Yu. A. 《Russian Engineering Research》2022,42(11):1110-1116
Russian Engineering Research - The stability of robot-mower motion in a specific direction is considered. The direction is regulated by means of an angular sensor and a programmable controller... 相似文献
135.
Revin A. A. Mikhaylova A. M. Konakov A. A. Tsyplenkov V. V. Shastin V. N. 《Semiconductors》2021,55(12):879-884
Semiconductors - The wave functions of electrons localized at P, As, and Sb shallow donors in Ge are calculated in the envelope function approximation taking into account valley–orbit... 相似文献
136.
Yang Hu Stephanie M. Sjoberg Chunpen Chen Amber L. Hauvermale Craig F. Morris Stephen R. Delwiche Ashley E. Cannon Camille M. Steber Zhiwu Zhang 《Comprehensive Reviews in Food Science and Food Safety》2022,21(3):2105-2117
This review examines the application, limitations, and potential alternatives to the Hagberg–Perten falling number (FN) method used in the global wheat industry for detecting the risk of poor end-product quality mainly due to starch degradation by the enzyme α-amylase. By viscometry, the FN test indirectly detects the presence of α-amylase, the primary enzyme that digests starch. Elevated α-amylase results in low FN and damages wheat product quality resulting in cakes that fall, and sticky bread and noodles. Low FN can occur from preharvest sprouting (PHS) and late maturity α-amylase (LMA). Moist or rainy conditions before harvest cause PHS on the mother plant. Continuously cool or fluctuating temperatures during the grain filling stage cause LMA. Due to the expression of additional hydrolytic enzymes, PHS has a stronger negative impact than LMA. Wheat grain with low FN/high α-amylase results in serious losses for farmers, traders, millers, and bakers worldwide. Although blending of low FN grain with sound wheat may be used as a means of moving affected grain through the marketplace, care must be taken to avoid grain lots from falling below contract-specified FN. A large amount of sound wheat can be ruined if mixed with a small amount of sprouted wheat. The FN method is widely employed to detect α-amylase after harvest. However, it has several limitations, including sampling variability, high cost, labor intensiveness, the destructive nature of the test, and an inability to differentiate between LMA and PHS. Faster, cheaper, and more accurate alternatives could improve breeding for resistance to PHS and LMA and could preserve the value of wheat grain by avoiding inadvertent mixing of high- and low-FN grain by enabling testing at more stages of the value stream including at harvest, delivery, transport, storage, and milling. Alternatives to the FN method explored here include the Rapid Visco Analyzer, enzyme assays, immunoassays, near-infrared spectroscopy, and hyperspectral imaging. 相似文献
137.
Alawiya Reslan Juliano G. Haddad Liadrine Moukendza Koundi Philippe Desprs Jean-Loup Bascands Gilles Gadea 《International journal of molecular sciences》2021,22(5)
Mosquito-borne Zika virus (ZIKV) became a real threat to human health due to the lack of vaccine and effective antiviral treatment. The virus has recently been responsible for a global outbreak leading to millions of infected cases. ZIKV complications were highlighted in adults with Guillain–Barré syndrome and in newborns with increasing numbers of congenital disorders ranging from mild developmental delays to fatal conditions. The ability of ZIKV to establish a long-term infection in diverse organs including the kidneys has been recently documented but the consequences of such a viral infection are still debated. Our study aimed to determine whether the efficiency of ZIKV growth in kidney cells relates to glucose concentration. Human kidney HK-2 cells were infected with different ZIKV strains in presence of normal and high glucose concentrations. Virological assays showed a decrease in viral replication without modifying entry steps (viral binding, internalization, fusion) under high glucose conditions. This decrease replication was associated with a lower virus progeny and increased cell viability when compared to ZIKV-infected HK-2 cells in normal glucose concentration. In conclusion, we showed for the first time that an elevated glucose level influences ZIKV replication level with an effect on kidney cell survival. 相似文献
138.
Kumar S. Ganesh Sridhar S. S. Hussain Azham Manikanthan S. V. Padmapriya T. 《Multimedia Tools and Applications》2022,81(7):9091-9109
Multimedia Tools and Applications - Discovering the relevant web services for specific applications in the dynamically changing business world becomes very critical. Researchers have used many... 相似文献
139.
Belcher Calvin H. Zheng Baolong MacDonald Benjamin E. Langlois Eric D. Lehman Benjamin Pearce Charles Delaney Robert Apelian Diran Lavernia Enrique J. Monson Todd C. 《Journal of Materials Science》2022,57(9):5518-5532
Journal of Materials Science - For transformers and inductors to meet the world’s growing demand for electrical power, more efficient soft magnetic materials with high saturation magnetic... 相似文献
140.
Mohammed Amin Salih Venkatachalam K. Hubálovský S. Trojovský P. Prabu P. 《Mobile Networks and Applications》2022,27(3):1050-1059
Mobile Networks and Applications - 5G/6G communication are first generation high speed wireless communication network which integrates the aerial data, terrestrial data and maritime data via... 相似文献