Mechanisms of resistance in a human cell line exposed to sequential topoisomerase poisoning

Camptothecins are a new class of anticancer drugs that target DNA topoisomerase I; current efforts are directed toward elucidating optimal combinations of these drugs with other antineoplastic agents. A rationale for the use of sequential therapy involving the combination of camptothecins with topoisomerase II-targeting drugs, such as etoposide, has arisen from observations of increased topoisomerase II protein levels in cell lines resistant to camptothecin. In an effort to understand potential mechanisms of resistance to this strategy, we developed a U-937 cell subline, denoted RERC, that is capable of surviving exposure to sequential topoisomerase poisoning. The RERC cells are 200-fold resistant to camptothecin, 8-fold resistant to etoposide, and 10-fold hypersensitive to cisplatin compared to the parental U-937 cells. Biochemical analyses indicate that the resistant phenotype involves alterations in both topoisomerase I and topoisomerase IIalpha. Topoisomerase I catalytic activity in the resistant cells is similar to that of the parental line but is resistant to camptothecin. Moreover, the resistant cells express a single mRNA species of topoisomerase I that codes for a mutation in codon 533. In addition, topoisomerase IIalpha protein levels are decreased 10-fold in the resistant line, coincident with a two-fold decrease in the expression of topoisomerase IIalpha mRNA. Collectively, these results indicate that resistance to sequential topoisomerase poisoning may involve a reduction in total cellular topoisomerase activity.     

单模光纤的传输衰减随温度变化的研究

本文从实验上测量了单模石英光纤在－50～120℃范围内的损耗。随着温度升高，光纤的损耗随之降低，在常温附近达到最小。其后损耗随着温度的继续升高而增加。损耗的变化主要是由于杂质的吸收和微弯损耗。本文对杂质的吸收以及微弯损耗进行了数值模拟，并且从理论上定性地解释了损耗对温度的依赖关系。     

Multiresolution analysis in extraction of reference lines fromdocuments with gray level background

Based on wavelets, a theoretical method has been developed to process multi-gray level documents. In this method, two-dimensional multiresolution analysis, a wavelet decomposition algorithm, and compactly supported orthonormal wavelets are used to transform a document image into sub-images. According to these sub-images, the reference lines of a multi-gray level document can be extracted, and knowledge about the geometric structure of the document can be acquired. Particularly, this approach is more efficient to process form documents with gray level background. Experiments indicate that this new method can be applied to process documents with promising results     

丙烷脱沥青装置技术改造

对玉门炼油化工总厂 0 .2 5Mt/a丙烷脱沥青装置的技术改造情况进行了较为详细的介绍 ,对改造前后的操作参数、产品质量和经济技术指标进行了对比。改造后加工能力提高到 0 .30Mt/a ,装置的操作弹性大大提高 ,产品质量优良 ,溶剂单耗由 16 .1kg/t降至 1.6 7kg/t,能耗由 2 4 49.3MJ/t降至 1172 .3MJ/t,年经济效率达 30 0 0× 10 4RMB $。     

加氢催化剂器外再生技术(HCRT)的开发与应用

淄博恒基化工有限公司开发的加氢催化剂器外再生技术 (HCRT) ,具有工艺合理、再生催化剂物性恢复好、活性损失少等特点 ,该技术已达到国外器外再生技术的水平。近年来已再生全国 19个炼油厂、2 3个品种的催化剂 12 95t,工业应用均取得满意结果     

肝脏占位的B超与MRI图像诊断分析

肝癌、肝血管瘤、肝囊肿是肝内常见占位性病变．笔者在临床工作中发现B超与MRI联合应用。其互补作用可提高肝脏占位的诊断率。本文通过对筛选出来的84例经B超与MRI诊断的肝脏占位病变进行分析。     

Adaptive predictive control algorithm based on Laguerre Functional Model

Laguerre Functional Model has many advantages such as good approximation capability for the variances of system time‐delay, order and other structural parameters, low computational complexity, and the facility of online parameter identification, etc., so this model is suitable for complex industrial process control. A series of successful applications have been gained in linear and non‐linear predictive control fields by the control algorithm based on Laguerre Functional Model, however, former researchers have not systemically brought forward the theoretical analyses of the stability, robustness, and steady‐state performance of this algorithm, which are the keys to guarantee the feasibility of the control algorithm fundamentally. Aimed at this problem, we introduce the principles of the Incremental Mode Linear Laguerre Predictive Control (IMLLPC) algorithm, and then systemically propose the theoretical analyses and proofs of the stability and robustness of the algorithm, in addition, we also put forward the steady‐state performance analysis. At last, the control performances of this algorithm on two different physical industrial plants are presented in detail, and a number of experimental results validate the feasibility and superiority of IMLLPC algorithm. Copyright © 2005 John Wiley & Sons, Ltd.     

Assessment of underground cable ratings based on distributed temperature sensing

Numerous power utilities have installed advanced distributed temperature sensing (DTS) systems to measure the temperature of underground cables. This paper presents a general procedure for real-time assessment of underground cable ratings based on DTS. After the identification and location of hot spots on a cable, the cable installation data, load, and DTS-recorded temperature over a period of time are used to estimate soil thermal parameters and assess cable ratings under normal and overloading conditions. In order to accurately estimate soil thermal parameters, a combined technique of the finite-element method and gradient-based optimization is used. The technique was validated in the laboratory and applied to a rating assessment of a 230-kV/500-MVA cable circuit. The assessed continuous and emergency ratings are helpful in improving the operation of this circuit.     

Price-maker型水电厂策略性报价

用古诺模型研究了Price-maker型水电厂在电力市场下的策略报价问题.将系统中各厂商的竞争简化为己方和虚拟对手组成的双人博弈,提出一种基于历史数据、分析对手行为的可行方法.该方法通过分析历史可获得数据,求取对手成本函数参数及系统的逆需求函数,由此获得合理的对手策略估计,并求取在此可能对手策略下的己方最优策略,从而达到己方收益最大的目标.