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91.
Natalia E. Llamas María S. Di Nezio Miriam E. Palomeque Beatriz S. Fernández Band 《Food Analytical Methods》2008,1(1):43-48
A simple and rapid analytical procedure for determination of saccharin (SAC) and acesulfame-K (AC-K) based on UV-vis measurements
and partial least squares (PLS) was proposed. Thus, an experimental design at levels 2 and 15 mg l−1 for SAC and 2 and 20 mg l−1 for AC-K was applied. Because real samples usually contain SAC and AC-K combined with aspartame (ASP), this interference
was also included in the model. The procedure was successfully applied for SAC and AC-K simultaneous determination in sweeteners
and fruit juice powders, without any separation step to remove ASP. The results were validated by using spiked samples, and
the obtained recoveries were satisfactory. 相似文献
92.
93.
The correlation between the ontogeny of Ubisch bodies and pollen development in Oxalis articulata was studied with Transmission Electron Microscopy (TEM). The ultrastructural changes observed during the different stages of development in the tapetal cells are related to Ubisch bodies, sporopollenin and pollen-kitt formation. The pro-orbicules have the appearance of lipid globuli and their formation is related to the endoplasmic reticulum of rough type (ERr). The lipid globules or pro-orbicules disappear in the mature Ubisch bodies, and the places that they occupied remain free of contents or with pollen-kitt. 相似文献
94.
95.
Paula Gregorio‐Godoy Jorge Pérez‐Juste Luis M. Liz‐Marzán Beatriz H. Juárez J. Ricardo Arias‐Gonzalez 《Small (Weinheim an der Bergstrasse, Germany)》2014,10(2):376-384
Temperature changes in the vicinity of a single absorptive nanostructure caused by local heating have strong implications in technologies such as integrated electronics or biomedicine. Herein, the temperature changes in the vicinity of a single optically trapped spherical Au nanoparticle encapsulated in a thermo‐responsive poly(N‐isopropylacrylamide) shell (Au@pNIPAM) are studied in detail. Individual beads are trapped in a counter‐propagating optical tweezers setup at various laser powers, which allows the overall particle size to be tuned through the phase transition of the thermo‐responsive shell. The experimentally obtained sizes measured at different irradiation powers are compared with average size values obtained by dynamic light scattering (DLS) from an ensemble of beads at different temperatures. The size range and the tendency to shrink upon increasing the laser power in the optical trap or by increasing the temperature for DLS agree with reasonable accuracy for both approaches. Discrepancies are evaluated by means of simple models accounting for variations in the thermal conductivity of the polymer, the viscosity of the aqueous solution and the absorption cross section of the coated Au nanoparticle. These results show that these parameters must be taken into account when considering local laser heating experiments in aqueous solution at the nanoscale. Analysis of the stability of the Au@pNIPAM particles in the trap is also theoretically carried out for different particle sizes. 相似文献
96.
Beatriz Jurado‐Sánchez Sirilak Sattayasamitsathit Wei Gao Luis Santos Yuri Fedorak Virendra V. Singh Jahir Orozco Michael Galarnyk Joseph Wang 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(4):499-506
Self‐propelled activated carbon‐based Janus particle micromotors that display efficient locomotion in environmental matrices and offer effective ‘on‐the‐fly’ removal of wide range of organic and inorganic pollutants are described. The new bubble‐propelled activated carbon Janus micromotors rely on the asymmetric deposition of a catalytic Pt patch on the surface of activated carbon microspheres. The rough surface of the activated carbon microsphere substrate results in a microporous Pt structure to provide a highly catalytic layer, which leads to an effective bubble evolution and propulsion at remarkable speeds of over 500 μm/s. Such coupling of the high adsorption capacity of carbon nanoadsorbents with the rapid movement of these catalytic Janus micromotors, along with the corresponding fluid dynamics and mixing, results in a highly efficient moving adsorption platform and a greatly accelerated water purification. The adsorption kinetics and adsorption isotherms have been investigated. The remarkable decontamination efficiency of self‐propelled activated carbon‐based Janus micromotors is illustrated towards the rapid removal of heavy metals, nitroaromatic explosives, organophosphorous nerve agents and azo‐dye compounds, indicating considerable promise for diverse environmental, defense, and public health applications. 相似文献
97.
Beatriz Galiana Carlos Algora Ignacio Rey-Stolle 《Solar Energy Materials & Solar Cells》2006,90(16):2589-2604
An extensive analysis of the front contact influence on concentrator GaAs solar cell performance has been carried out. The fill factor, open circuit voltage and efficiency have been calculated by varying the front contact specific resistance and the metal sheet resistance for 500X, 1000X and 2000X. An optimum front grid design has also been developed. The simulations have been carried out using a 3D model based on distributed circuit units, and by a classic lumped model, showing the need to use distributed models to achieve an accurate concentrator solar cell modeling as well as a precise front grid design. 相似文献
98.
Maria Beatriz Takahashi Aline Florencio Teixeira Ana Lucia Tabet Oller Nascimento 《International journal of molecular sciences》2022,23(24)
Leptospirosis is a neglected infectious disease with global impact on both humans and animals. The increase in urban development without sanitation planning is one of the main reasons for the disease spreading. The symptoms are similar to those of flu-like diseases, such as dengue, yellow fever, and malaria, which can result in a misleading clinical diagnosis. The characterization of host–pathogen interactions is important in the development of new vaccines, treatments, and diagnostics. However, the pathogenesis of leptospirosis is not well understood, and many gaps remain to be addressed. Here, we aimed to determine if Leptospira strains, virulent, culture-attenuated, and saprophytic, and the major outer membrane proteins OmpL37, OmpL1, LipL21, LipL41, and LipL46 are able to adhere to different endothelial, epithelial and fibroblast cell lines in vitro. We showed that virulent leptospires robustly bind to all cells compared to the culture-attenuated and saprophytic lines. The recombinant proteins exhibited certain adhesion, but only OmpL1 and LipL41 were able to bind to several cell lines, either in monolayer or in cell suspension. Blocking OmpL1 with polyclonal antibodies caused a decrease in bacterial binding to cells, contrasting with an increase observed when anti-LipL41 antibodies were used. The adhesion of OmpL1 to HMEC-1 and EA.hy926 was inhibited when cells were pre-incubated with collagen IV, suggesting that both compete for the same cell receptor. We present here for the first time the interaction of five leptospiral outer membrane proteins with several cell lines, and we conclude that LipL41 and OmpL1 may have an impact on leptospiral adhesion to mammalian cells and may mediate the colonization process in leptospiral pathogenesis. 相似文献
99.
100.
Beatriz M. Oliveira Lucas Ferreira de Almeida Amanda L. Deluque Claudia S. Souza Ana Lívia D. Maciel Heloísa D. C. Francescato Roberto S. Costa Cleonice Giovanini Francisco Jos A. de Paula Terezila M. Coimbra 《International journal of molecular sciences》2022,23(24)
Cisplatin treatment is one of the most commonly used treatments for patients with cancer. However, thirty percent of patients treated with cisplatin develop acute kidney injury (AKI). Several studies have demonstrated the effect of bioactive vitamin D or calcitriol on the inflammatory process and endothelial injury, essential events that contribute to changes in renal function and structure caused by cisplatin (CP). This study explored the effects of calcitriol administration on proximal tubular injury, oxidative stress, inflammation and vascular injury observed in CP-induced AKI. Male Wistar Hannover rats were pretreated with calcitriol (6 ng/day) or vehicle (0.9% NaCl). The treatment started two weeks before i.p. administration of CP or saline and was maintained for another five days after the injections. On the fifth day after the injections, urine, plasma and renal tissue samples were collected to evaluate renal function and structure. The animals of the CP group had increased plasma levels of creatinine and of fractional sodium excretion and decreased glomerular filtration rates. These changes were associated with intense tubular injury, endothelial damage, reductions in antioxidant enzymes and an inflammatory process observed in the renal outer medulla of the animals from this group. These changes were attenuated by treatment with calcitriol, which reduced the inflammation and increased the expression of vascular regeneration markers and antioxidant enzymes. 相似文献