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991.
Environmental education and computers? That was traditionally seen as an antagonism. But environmental educators who compete for attention and face new challenges in an age of mobile devices, have begun to explore the opportunities that mobile computers may offer in supporting environmental learning experiences. This study investigates the impact of a mobile guide system on different parameters of environmental literacy in comparison to traditional instruments of environmental education (i.e. brochure, human guide). In a field experiment at a floodplain conservation site, 185 school children and 76 adults participated in a guided tour using different media. Despite the novelty of mobile devices and usability issues associated with the prototype mobile nature guide, participants using the computer-assisted medium achieved similar results concerning environmental literacy components. The computer as mobile guide can lead to an increase in environmental knowledge and in case of the children it can increase their motivation to engage in environmental education activities. 相似文献
992.
Erin E Powell Gordon A Hill Bernhard HJ Juurlink D Julie Carrier 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2005,80(9):992-997
Glucoraphanin is a potentially valuable plant compound that has shown efficacy in the treatment of hypertension and the removal of carcinogens in animals. Our recent work determined the optimum extraction conditions for the recovery of high amounts of glucoraphanin from the noxious weed Cardaria draba in a single‐staged batch extractor. In this study, a multiple contact, countercurrent flow extraction process was used to achieve further improvements in glucoraphanin recovery. The yield increased by 1.5 times when compared with a single, batch contact extraction. A three‐stage process was sufficient to extract over 90% of the glucoraphanin from C draba at a dry weight plant loading of 50 g dm?3. The experimental results at several solids loadings agreed with the leaching theory and the theoretical model was used to predict the number of stages needed to extract all the glucoraphanin from C draba at high solids loadings. Finally the efficacy and toxicity of the crude glucoraphanin extracts were tested using hepatoma cells. The efficacy was found to be much higher than single‐stage extracts, confirming that cell efficacy is related to the increased quantity of glucoraphanin extracted due to countercurrent operation. The crude extract demonstrated negligible acute toxicity to the hepatoma cells. Copyright © 2005 Society of Chemical Industry 相似文献
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995.
Rahmer J Weizenecker J Gleich B Borgert J 《IEEE transactions on medical imaging》2012,31(6):1289-1299
Magnetic particle imaging (MPI) is a new tomographic imaging approach that can quantitatively map magnetic nanoparticle distributions in vivo. It is capable of volumetric real-time imaging at particle concentrations low enough to enable clinical applications. For image reconstruction in 3-D MPI, a system function (SF) is used, which describes the relation between the acquired MPI signal and the spatial origin of the signal. The SF depends on the instrumental configuration, the applied field sequence, and the magnetic particle characteristics. Its properties reflect the quality of the spatial encoding process. This work presents a detailed analysis of a measured SF to give experimental evidence that 3-D MPI encodes information using a set of 3-D spatial patterns or basis functions that is stored in the SF. This resembles filling 3-D k-space in magnetic resonance imaging, but is faster since all information is gathered simultaneously over a broad acquisition bandwidth. A frequency domain analysis shows that the finest structures that can be encoded with the presented SF are as small as 0.6 mm. SF simulations are performed to demonstrate that larger particle cores extend the set of basis functions towards higher resolution and that the experimentally observed spatial patterns require the existence of particles with core sizes of about 30 nm in the calibration sample. A simple formula is presented that qualitatively describes the basis functions to be expected at a certain frequency. 相似文献
996.
Exposure of an adult population to perfluorinated substances using duplicate diet portions and biomonitoring data 总被引:4,自引:0,他引:4
Fromme H Schlummer M Möller A Gruber L Wolz G Ungewiss J Böhmer S Dekant W Mayer R Liebl B Twardella D 《Environmental science & technology》2007,41(22):7928-7933
Because dietary intake is supposed to be an important route of human exposure we quantified the dietary intake of perfluorooctane sulfonate (PFOS), perfluorooctanoate (PFOA), perfluorohexane sulfonate (PFHxS), perfluorohexanoate (PFHxA), and perfluorooctane sulfonamide (PFOSA) using 214 duplicate diet samples. The study was carried out with a study population of 15 female and 16 male healthy subjects aged 16-45 years. The participants collected daily duplicate diet samples over seven consecutive days in 2005. Duplicate samples were homogenized and their ultrasonic extracts were cleaned up by SPE and subjected to HPLC-ESI-MS/MS. In addition, individual intakes were estimated based on blood levels of PFOS and PFOA using a pharmacokinetic model. Blood samples were collected once during the sampling period. The median (90th percentile) daily dietary intake of PFOS and PFOA was 1.4 ng/kg b.w. (3.8 ng/kg b.w.) and 2.9 ng/kg b.w. (8.4 ng/kg b.w.), respectively. PFHxS and PFHxA could be detected only in some samples above detection limit with median (maximum) daily intakes of 2.0 ng/kg b.w. (4.0 ng/kg b.w.) and 4.3 ng/kg b.w. (9.2 ng/kg b.w.), respectively. Because PFOSA could not be detected above the limit of detection of 0.2 ng/g f.w. this indirect route of exposure seems to be of less significance. Overall, the results of this study demonstrate that the German population is exposed to PFOS and PFOA, but the median dietary intake did not reach the recommended tolerable daily intake by far. Biomonitoring data predict an exposure in a comparable range. We suppose that, normally, food intake is the main source of exposure of the general population to PFOS and PFOA. 相似文献
997.
Liao Wang Yisheng Chen Zhiyang Ye Bernhard Hellmann Xueming Xu Zhengyu Jin Qinqin Ma Na Yang Fengfeng Wu Yamei Jin 《Food Analytical Methods》2018,11(11):3170-3178
The cytotoxicity of phenolic antioxidants had attracted marked attention, posing serious challenges to food safety. This paper presented a screening method for two major phenolic antioxidants (butylated hydroxytoluene and tert-butylhydroquinone) added in edible oils. To specifically visualize the targeted compounds after developing with toluene/ethyl acetate/methanol 8:1:1 (v/v/v) to 70 mm solvent front, the plate was subjected to a standardized 1,1-diphenyl-2-picrylhydrazyl assay. In addition to synoptical eye inspection, accurate quantification was realized by modified densitometric measurements: fluorescence mode, excitation wavelength 530 nm (D2 and W lamp) without optical filter, which offered satisfactory sensitivity (8.5–17.5 mg/kg) and acceptable linearity (R2?>?0.999 within 50–200 ng/zone). Moreover, the established method was validated with edible oil samples, against EU Directive 2006/52/EC. Apart from that, the unambiguous confirmation of positive results was conveniently achieved by TLC-MS interface-mediated mass spectrometry. Featuring the merits of screening conception, the proposed method not only reached the goal of accurate quantification and conclusive identification of multi-phenolic antioxidants, but also excellently balanced the simplicity, detectability, and throughput of the screening workup. Therefore, it might be an attractive alternative to conventional methods. 相似文献
998.
999.
Bastian Tewes Dr. Bastian Frehland Dirk Schepmann Dr. Kai‐Uwe Schmidtke Thomas Winckler Prof. Dr. Bernhard Wünsch Prof. Dr. 《ChemMedChem》2010,5(5):687-695
Cleavage and reconstitution of a bond in the piperidine ring of ifenprodil ( 1 ) leads to 7‐methoxy‐2,3,4,5‐tetrahydro‐1H‐3‐benzazepin‐1‐ols, a novel class of NR2B‐selective NMDA receptor antagonists. The secondary amine 7‐methoxy‐2,3,4,5‐tetrahydro‐1H‐3‐benzazepin‐1‐ol ( 12 ), which was synthesized in six steps starting from 2‐phenylethylamine 3 , represents the central building block for the introduction of several N‐linked residues. A distance of four methylene units between the basic nitrogen atom and the phenyl residue in the side chain results in high NR2B affinity. The 4‐phenylbutyl derivative 13 (WMS‐1405, Ki=5.4 nM ) and the conformationally restricted 4‐phenylcyclohexyl derivative 31 (Ki=10 nM ) represent the most potent NR2B ligands of this series. Whereas 13 shows excellent selectivity, the 4‐phenylcyclohexyl derivative 31 also interacts with σ1 (Ki=33 nM ) and σ2 receptors (Ki=82 nM ). In the excitotoxicity assay the phenylbutyl derivative 13 inhibits the glutamate‐induced cytotoxicity with an IC50 value of 360 nM , indicating that 13 is an NMDA antagonist. 相似文献
1000.
Elvira Ruppel Dr. Bernhard Aÿ Dr. Prisca Boisguerin Sabine Dölle Prof. Dr. Margitta Worm Dr. Rudolf Volkmer 《Chembiochem : a European journal of chemical biology》2010,11(16):2283-2293
Celery is a frequent cause of food allergy in pollen‐sensitized patients and can induce severe allergic reactions. Clinical symptoms cannot be predicted by skin prick tests (SPTs) or by determining allergen‐specific immunoglobulin E (IgE). Our aim was to identify specific IgE binding peptides by using an array technique. For our study, the sera of 21 patients with positive double‐blind, placebo‐controlled food challenge (DBPCFC) to celery, as well as the sera of 17 healthy patients were used. Additionally, all patients underwent skin tests along with determinations of specific IgE binding. The major allergen of celery Api g 1.0101 (Apium graveolens) was synthesized as an array of overlapping peptides and probed with the patients' sera. We developed an improved immunoassay protocol by investigating peptide lengths, peptide densities, incubation parameters, and readout systems, which could influence IgE binding. Sera of celery‐allergic patients showed binding to three distinct regions of Api g 1.0101. The region including amino acids 100 to 126 of Api g 1.0101 is the most important region for IgE binding. This region caused a fivefold higher binding of IgE from the sera of celery‐allergic patients compared to those of healthy individuals. In particular, one peptide (VLVPTADGGSIC) was recognized by all sera of celery‐allergic patients. In contrast, no binding to this peptide was detected in sera of the healthy controls. Our improved assay strategy allows us to distinguish between celery‐allergic and healthy individuals, but needs to be explored in a larger cohort of well‐defined patients. 相似文献