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11.
Lorenzo Ntogramatzidis Michael Cantoni Ran Yang 《Multidimensional Systems and Signal Processing》2008,19(3-4):449-475
In this paper we consider the problem of internally and externally stabilising controlled invariant and output-nulling subspaces for two-dimensional (2-D) Fornasini–Marchesini models, via static feedback. A numerically tractable procedure for computing a stabilising feedback matrix is developed via linear matrix inequality techniques. This is subsequently applied to solve, for the first time, various 2-D disturbance decoupling problems subject to a closed-loop stability constraint. 相似文献
12.
Analysis of PCR-based methods for characterization of Listeria monocytogenes strains isolated from different sources 总被引:3,自引:0,他引:3
Cocolin L Stella S Nappi R Bozzetta E Cantoni C Comi G 《International journal of food microbiology》2005,103(2):167-178
Listeria monocytogenes strains, isolated from various sources (food, environment, and animals), were used to test different PCR-based methods to investigate their capability to define the strain origin. RAPD-PCR with three primers and the SAU-PCR method, in which the DNA was first digested with the Sau3A restriction endonuclease and then amplified with a primer designed on the restriction site, were carried out, and the profiles obtained were used to perform cluster analysis. Based on the cluster analysis of Listeria spp. strains, obtained from international collections, the coefficient of similarity was selected. The results obtained showed that the methods tested in the study gave different levels of differentiation between the strains tested. The RAPD protocol using the P1254 primer and the SAU-PCR gave appreciable results only for strains isolated from animals and from a food processing plant in two different periods of the year 2003. Better differentiation was observed using the RAPD-PCR with primer D8635. As a matter of fact, it was able to distinguish L. monocytogenes obtained from different species of animals, different food samples and strains from the same production plant isolated in different periods of the year. Also primer M13 gave positive results, but the coefficient of similarity to use had to be increased to 80%. On the basis of the results observed, RAPD-PCR with primers D8635 and M13 should be considered reliable tools for epidemiological investigations focusing on L. monocytogenes. 相似文献
13.
1. The effects of three different NO donors on tert-butylhydroperoxide (tB-OOH)-induced DNA cleavage and toxicity were investigated in U937 cells. 2. Treatment with S-nitroso-N-acetyl-penicillamine (SNAP, 1-30 microM), while not in itself DNA-damaging, potentiated the DNA strand scission induced by 200 microM tB-OOH in a concentration-dependent fashion. The enhancing effects of SNAP were observed with two different techniques for the assessment of DNA damage. Decomposed SNAP was inactive. S-nitrosoglutathione (GSNO, 300 microM) and (Z)-1-[(2-aminoethyl)-N-(2-ammonioethyl) amino]diazen-1-ium-1,2-diolate (DETA-NO, 1 mM) also increased DNA cleavage generated by tB-OOH and these responses, as well as that mediated by SNAP, were prevented by the NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazolin-1-oxyl-3-oxide (PTIO). 3. SNAP neither inhibited catalase activity nor increased the formation of DNA lesions in cells exposed to H2O2. Furthermore, SNAP did not affect the rate of rejoining of the DNA single strand breaks generated by tB-OOH. 4. Under the conditions utilized in the DNA damage experiments, treatment with tB-OOH alone or associated with SNAP did not cause cell death. However, SNAP as well as GSNO markedly reduced the lethal response promoted by millimolar concentrations of tB-OOH and these effects were abolished by PTIO. Decomposed SNAP was inactive. 5. It is concluded that low levels of NO donors, which probably release physiological concentrations of NO, enhance the accumulation of DNA single strand breaks in U937 cells exposed to tB-OOH. This NO-mediated effect appears to (a) not depend on inhibition of either DNA repair (which would increase the net accumulation of DNA lesions by preventing DNA single strand break removal) or catalase activity (which would also enhance the net accumulation of DNA lesions since H2O2 is one of the species mediating the tB-OOH-induced DNA cleavage) and (b) be caused by enforced formation of tB-OOH-derived DNA-damaging species. In contrast to these results, similar concentrations of NO prevented cell death caused by millimolar concentrations of tB-OOH. Hence, DNA single strand breakage generated by tB-OOH in the absence or presence of NO does not represent a lethal event. 相似文献
14.
15.
On‐Chip Magnetic Platform for Single‐Particle Manipulation with Integrated Electrical Feedback
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Marco Monticelli Andrea Torti Matteo Cantoni Daniela Petti Edoardo Albisetti Alessandra Manzin Erica Guerriero Roman Sordan Giacomo Gervasoni Marco Carminati Giorgio Ferrari Marco Sampietro Riccardo Bertacco 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(7):921-929
Methods for the manipulation of single magnetic particles have become very interesting, in particular for in vitro biological studies. Most of these studies require an external microscope to provide the operator with feedback for controlling the particle motion, thus preventing the use of magnetic particles in high‐throughput experiments. In this paper, a simple and compact system with integrated electrical feedback is presented, implementing in the very same device both the manipulation and detection of the transit of single particles. The proposed platform is based on zig‐zag shaped magnetic nanostructures, where transverse magnetic domain walls are pinned at the corners and attract magnetic particles in suspension. By applying suitable external magnetic fields, the domain walls move to the nearest corner, thus causing the step by step displacement of the particles along the nanostructure. The very same structure is also employed for detecting the bead transit. Indeed, the presence of the magnetic particle in suspension over the domain wall affects the depinning field required for its displacement. This characteristic field can be monitored through anisotropic magnetoresistance measurements, thus implementing an integrated electrical feedback of the bead transit. In particular, the individual manipulation and detection of single 1‐μm sized beads is demonstrated. 相似文献
16.
In envelope-constrained (EC) filter design, the effect of input noise is minimised subject to the constraint that the filter's response to a specified signal fits into a prescribed envelope. The continuous-time version of this problem has been addressed using orthonormal analogue filters. The paper addresses the EC filter-design problem using a filter comprised of an A/D converter, a digital processor, a D/A converter and an analogue interpolation filter. Numerical results are presented for a number of design examples 相似文献
17.
Dam H.H. Cantoni A. Nordholm S. Teo K.L. 《IEEE transactions on circuits and systems. I, Regular papers》2006,53(5):1108-1118
In this paper, we formulate a general design of transversal filter structures with maximum relative passband-to-stopband energy ratio subject to complex frequency response constraints in the passband and the stopband as well as additional constraints such as constraints. These constraints are important for applications where the suppression of noise at certain frequencies are important. Additional constraints are introduced allowing approximately linear phase and constant group delay in the passband. For a given set of basis functions, the design problem can be formulated as a semi-infinite quadratic optimization problem in the filter coefficients, which are the decision variables to be optimized. In this paper, we focus on the design of digital Laguerre filter and digital finite impulse response (FIR) filter structures. A modified bridging algorithm is developed for searching for the optimum pole of the Laguerre filters. Design examples are given to demonstrate the effectiveness of the proposed algorithm. 相似文献
18.
Cantoni A. Walker J. Tomlin T.-D. 《IEEE transactions on circuits and systems. I, Regular papers》2007,54(5):1032-1040
We characterize a proposed metastability measurement system in which asynchronous data input and sampling clock frequencies trigger metastability. We develop an equation describing the time interval between data and clock inputs for practical frequencies and show that it takes on discrete values in the absence of jitter and that the presence of jitter perturbs these values. Finally, we present experimental results supporting our characterization 相似文献
19.
The jitter generated by two different approaches to synchronization of an external timing source is analyzed. The two approaches are: the use of conventional stuffing and the use of a simpler frame sampling synchronizer. It is shown that these two approaches have the same jitter power density spectrum. The result is obtained using a new time domain method developed in the paper. Exact expressions for the jitter on the synchronized external timing source are obtained. Furthermore, the effect of quantization in the conventional stuffing synchronizer on the jitter expressions obtained is also explored. Jitter generated by the frame sampling synchronizer is also analyzed using the time domain method, which again produces an exact expression. In the case where certain parameters of the two approaches are related, the location of spectral lines in their respective jitter spectra is shown to be identical. It is also shown that both approaches to synchronization introduce the so-called waiting-time jitter 相似文献
20.
Multiprocessor computing for images 总被引:1,自引:0,他引:1
Cantoni V. Levialdi S. 《Proceedings of the IEEE. Institute of Electrical and Electronics Engineers》1988,76(8):959-969
A review of image-processing systems developed until now is given, highlighting the weak points of such systems and the trends that have dictated their evolution through the years producing different generations of machines. Each generation may be characterized by the hardware architecture, the programmability features and the relative application areas. The need for multiprocessing hierarchical systems is discussed, focusing on pyramidal architectures. Their computational paradigms, their virtual and physical implementation, their programming and languages, are discussed 相似文献