Rapid and sensitive detection of Salmonella Typhimurium on eggshells by using wireless biosensors

This article presents rapid, sensitive, direct detection of Salmonella Typhimurium on eggshells by using wireless magnetoelastic (ME) biosensors. The biosensor consists of a freestanding, strip-shaped ME resonator as the signal transducer and the E2 phage as the biomolecular recognition element that selectively binds with Salmonella Typhimurium. This ME biosensor is a type of mass-sensitive biosensor that can be wirelessly actuated into mechanical resonance by an externally applied timevarying magnetic field. When the biosensor binds with Salmonella Typhimurium, the mass of the sensor increases, resulting in a decrease in the sensor's resonant frequency. Multiple E2 phage-coated biosensors (measurement sensors) were placed on eggshells spiked with Salmonella Typhimurium of various concentrations (1.6 to 1.6 × 10(7) CFU/cm(2)). Control sensors without phage were also used to compensate for environmental effects and nonspecific binding. After 20 min in a humidity-controlled chamber (95%) to allow binding of the bacteria to the sensors to occur, the resonant frequency of the sensors was wirelessly measured and compared with their initial resonant frequency. The resonant frequency change of the measurement sensors was found to be statistically different from that of the control sensors down to 1.6 × 10(2) CFU/cm(2), the detection limit for this work. In addition, scanning electron microscopy imaging verified that the measured resonant frequency changes were directly related to the number of bound cells on the sensor surface. The total assay time of the presented methodology was approximately 30 min, facilitating rapid detection of Salmonella Typhimurium without any preceding sampling procedures.     

L-亮氨酸产生菌的选育及其发酵条件优化

以黄色短杆菌TQ980 6(Met- +IleL+2 TAr+α ABr+β HLr+SGr+Rifr3 0 0 μg/mL)为出发菌株 ,经硫酸二乙酯、紫外线分别与LiCl复合诱变 ,选育出一株利福平抗性为 80 0 μg/mL的L 亮氨酸产生菌TK0 3 0 3。通过对其摇瓶发酵条件的研究和优化 ,产量由出发菌株TQ980 6的 2 2 7g/L提高至目的突变株TK0 3 0 3的 2 8 3g/L。     

东北亚地区铜矿资源与供需概况

我国是全球最大的铜消费国,铜矿资源供需矛盾问题日益突出,需大量从国外进口。本文通过对比分析东北亚地区俄罗斯、蒙古、朝鲜、韩国、日本和我国东北地区的铜矿资源概况和供需情况,明确了东北亚地区境外铜矿资源在俄罗斯、蒙古和朝鲜相对丰富,富集区分布在俄罗斯西伯利亚地区和乌拉尔地区、蒙古国北部的额尔登特地区以及与中国接壤的南戈壁和东戈壁省、朝鲜惠山-利原地区,我国与日本、韩国是东北亚地区主要的铜消费区。结合我国铜矿资源对外依存度过高的现状,发挥我国东北地区的地域优势,加强区域矿业合作,勘探、开发、利用东北亚地区铜矿资源,加大地质矿产国际合作的科研投入,深入交流与研究,将有利于缓解我国铜矿资源供需不平衡的矛盾。     

Quantification and reduction of organic sulfur compound formation in a commercial wood pulping process

This study experimentally quantified the formation of organic sulfur compounds in a commercial SuperBatch kraft pulping process using a laboratory pilot-scale digester. The results indicate that wood chips not only can adsorb HS- but also methyl mercaptan (MM) in the black liquor used for pretreatment during the two pretreatment stages. The absorption rate of MM is much faster than that of HS-. In the third stage of SuperBatch pulping, the rate of formation of MM and dimethyl sulfide (DMS) is very similar to that found in conventional batch kraft pulping processes. The study examined the applicability of the phase transition cooking (PTC) concept for reducing organic sulfur compounds in pulping, previously developed in laboratory batch pulping using a bomb-type digester, in a SuperBatch process. It was confirmed that there is a phase transition point (PTP) corresponding to PTC in SuperBatch pulping beyond which further delignification significantly increases the formation of volatile organic sulfur compounds. The results indicate that a 40% reduction of TRS formation can be achieved by using PTC.     

结合局部全局特征与多尺度交互的三维多器官分割网络

目的 高度适形放射治疗是常用的癌症治疗方法,该方法的有效性依赖于对癌组织和周边多个危及器官（organ at risk,OAR）解剖结构的精确刻画,因此研究三维图像多器官的高精度自动分割具有重要意义。以视觉Transformer(vision Transformer,ViT)和卷积神经网络（convolutional neural network,CNN）结合为代表的三维医学图像分割方法表现出了丰富的应用优势。然而,这类方法往往忽略同一尺度内和不同尺度间的信息交互,使得CNN和ViT特征的提取和融合受限。本文提出一种端到端多器官分割网络LoGoFUNet(local-global-features fusion UNet),旨在应对现有方法的缺陷。方法 首先,针对单一器官分割,提出在同一尺度下并行提取并融合CNN和ViT特征的LoGoF(local-global-features fusion)编码器,并构建了一个端到端的三维医学图像分割多尺度网络M0。此外,考虑到器官内部以及器官之间的相互关系,该方法在M0网络的基础上设计并引入了多尺度交互（multi-scale interacti...     

Repetitive Sequence Barcode Probe for Karyotype Analysis in Tripidium arundinaceum

The barcode probe is a convenient and efficient tool for molecular cytogenetics. Tripidium arundinaceum, as a polyploid wild allied genus of Saccharum, is a useful genetic resource that confers biotic and abiotic stress resistance for sugarcane breeding. Unfortunately, the basic cytogenetic information is still unclear due to the complex genome. We constructed the Cot-20 library for screening moderately and highly repetitive sequences from T. arundinaceum, and the chromosomal distribution of these repetitive sequences was explored. We used the barcode of repetitive sequence probes to distinguish the ten chromosome types of T. arundinaceum by fluorescence in situ hybridization (FISH) with Ea-0907, Ea-0098, and 45S rDNA. Furthermore, the distinction among homology chromosomes based on repetitive sequences was constructed in T. arundinaceum by the repeated FISH using the barcode probes including Ea-0663, Ea-0267, EaCent, 5S rDNA, Ea-0265, Ea-0070, and 45S rDNA. We combined these probes to distinguish 37 different chromosome types, suggesting that the repetitive sequences may have different distributions on homologous chromosomes of T. arundinaceum. In summary, this method provide a basis for the development of similar applications for cytogenetic analysis in other species.     

基于工作流的农电缺陷管理评估系统研究与设计

缺陷管理是农电管理中重要的一部分,为提高农电缺陷管理水平,研究设计了一套农电缺陷管理评估系统.系统运用工作流技术对缺陷管理中的流程化业务实现闭环管理,使各部分业务间环环相扣,规范了业务流程;采用从工作流中抽取指标数据的方法对下属单位的缺陷管理水平进行评价,为市级供电公司与县级供电公司各提出-套评价指标体系.     

滚子链链板理论应力集中系数的研究

通过有限元法理论计算、光弹试验，研究了滚子链链板理论应力集中系数问题。结果表明，链板理论应力集中系数与链板节距和外载荷大小无关，只与腰部宽度有关，并给出了按链板腰宽计算理论应力集中系数的算式。     

求解非线性互补问题的光滑化拟牛顿法

利用光滑Chen Harker KanzowSmale函数和Robinson正则法,将非线性互补问题转化为与之等价的光滑非线性方程组,并基于无导数线搜索技术提出了一种新的求解P0非线性互补问题的光滑化拟牛顿法.在一定条件下获得了算法的全局收敛性,数值实验表明该算法是有效的.