LDL binding to lipid emulsion particles: effects of incubation duration,temperature, and addition of plasma subfractions

Lipid emulsions used in parenteral nutrition interact with lipoproteins leading to exchanges of lipids and acquisition of several apolipoproteins (apo). It has been previously observed that, during in vitro incubation of emulsions with purified LDL, a variable fraction of LDL binds to TG-rich emulsion particles. The purpose of this study was to better characterize such an interaction. Two emulsions containing 20% soybean oil (Endolipid, B. Braun AG, Melsungen, Germany) or fish oil were incubated with LDL, either alone or in the presence of various plasma subfractions, for different durations and at different temperatures. The fraction named M-LE (containing TG-rich particles modified after incubation) was separated by ultracentrifugation or gel filtration chromatography, and the apoB content was measured as an index of LDL binding to TG-rich emulsion particles. The formation of such complexes was visualized by freeze-fracture electron microscopy. LDL binding was not influenced by the method used for M-LE isolation. Binding occurred quickly, did not increase with prolonged incubation, was inversely related to increasing incubation or ultracentrifugation temperature, and withstood 40 h of ultracentrifugation at 163,000 x g. The presence of glycerol or excess phospholipids in the emulsion did not markedly affect the formation of the complexes. In contrast, adding very small amounts of lipoprotein-poor plasma (d > 1.210 g/mL) or HDL markedly reduced the process, and albumin had no effect. The TG composition of the emulsion influenced the binding of LDL to TG-rich particles, since more apoB was found in M-LE from fish oil than from soybean oil emulsion.     

A flexible real-time scheduling abstraction: design and implementation

An evolution is happening in the way that operating systems support the real-time requirements of their applications. The need to run real-time applications such as multimedia in the same environment as complex non-real-time servers and applications has motivated much interest in restructuring existing operating systems. Many issues related to thread scheduling and synchronization have been investigated. However, little consideration has been given to the flexibility and modularity required in the support of application-level scheduling needs, although it is well known that application requirements are diverse. In this paper, we describe a real-time scheduling abstraction which provides modularity and flexibility to the scheduling support of operating systems. Our design has been implemented using the Mach 3.0 kernel and a locally developed multiprocessor kernel (the r-kernel) as development platforms. © 1997 John Wiley & Sons, Ltd.     

Experiments on two-phase flow in hydraulic jump on pebbled rough bed: Part 1–Turbulence properties and particle chord time and length

This study reported and discussed turbulence characteristics, such as turbulence intensity, correlation time scales, and advective length scales. The characteristic air–water time scale, including the particle chord time and length and their probability density functions (PDFs), was investigated. The results demonstrated that turbulence intensity was relatively greater on a rough bed in the roller length, whereas further downstream, the decay rate was higher. In addition, the relationship between turbulence intensity and dimensionless bubble count rate reflected an increase in turbulence intensity associated with the number of entrained particles. Triple decomposition analysis (TDA) was performed to determine the contributions of slow and fast turbulent components. The TDA results indicated that, regardless of bed type and inflow conditions, the sum of the band-pass (T'_u) and high-pass (T″_u) filtered turbulence intensities was equal to the turbulence intensity of the raw signal data (T_u). T″_u highlighted a higher turbulence intensity and larger vorticities on the rough bed for an identical inflow Froude number. Additional TDA results were presented in terms of the interfacial velocity, auto- and cross-correlation time scales, and longitudinal advection length scale, with the effects of low- and high-frequency signal components on each highlighted parameter. The analysis of the air chord time indicated an increase in the proportion of small bubbles moving downstream. The second part of this research focused on the basic properties of particle grouping and clustering.     

Experiments on two-phase flow in hydraulic jump on pebbled rough bed: Part 2–Bubble clustering

A survey on bubble clustering in air–water flow processes may provide significant insights into turbulent two-phase flow. These processes have been studied in plunging jets, dropshafts, and hydraulic jumps on a smooth bed. As a first attempt, this study examined the bubble clustering process in hydraulic jumps on a pebbled rough bed using experimental data for 1.70 < Fr₁ < 2.84 (with Fr₁ denoting the inflow Froude number). The basic properties of particle grouping and clustering, including the number of clusters, the dimensionless number of clusters per second, the percentage of clustered bubbles, and the number of bubbles per cluster, were analyzed based on two criteria. For both criteria, the maximum cluster count rate was greater on the rough bed than on the smooth bed, suggesting greater interactions between turbulence and bubbly flow on the rough bed. The results were consistent with the longitudinal distribution of the interfacial velocity using one of the criteria. In addition, the clustering process was analyzed using a different approach: the interparticle arrival time of bubbles. The comparison showed that the bubbly flow structure had a greater density of bubbles per unit flux on the rough bed than on the smooth bed. Bed roughness was the dominant parameter close to the jump toe. Further downstream, Fr₁ predominated. Thus, the rate of bubble density decreased more rapidly for the hydraulic jump with the lowest Fr₁.     

Scalability support for multiparty multimedia communications

In this paper, we propose a new communication abstraction known as the group channel which facilitates and supports the implementation of multiparty interactive multimedia (MIM) applications such as video conferencing. The group channel is a high-level abstraction for group communication. The credit scheme and the dynamic bandwidth calibration scheme are provided as an integral part of the group channel service for allocating network bandwidth dynamically as participants join and leave the group channel. The multimedia transport protocol (MTP) is proposed as a realization of the group channel service in the ATM network. Its prototype implementation and a simple multiparty video-conferencing application built on top of the MTP prototype are described in this paper. Our results show that the group channel is capable of guaranteeing the performance of MIM applications irrespective of the group size and differences in workstation speeds.