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21.
The GM2 activator protein is a small monomeric protein containing a single site for Asn-linked glycosylation. Its only proven in vivo function is to act as a substrate specific cofactor for the hydrolysis of GM2 ganglioside by lysosomal beta-hexosaminidase A. However, we and others have shown it can act as a general glycolipid transporter at neutral pH in vitro. Any other possible in vivo functions would require that some of the newly synthesized activator molecules not be targeted to the lysosome. The lysosomal targeting mechanism for the activator has not been conclusively identified. While earlier reports suggested that it is likely through the mannose-6-phosphate receptor, another more recent report demonstrated that deficient human cells could recapture nonglycosylated, bacterially produced activator, suggesting its use of an alternate targeting pathway. Here, we demonstrate that the mannose-6-phosphate pathway is likely the major intracellular, biosynthetic route to the lysosome, as well as a high affinity recapture pathway for the endocytosis of activator protein from extracellular fluids. Additionally, we show that there exists a second lower affinity recapture pathway that requires its native protein structure, is carbohydrate independent, and likely does not involve its ability to bind glycosphingolipids in the plasma membrane. Finally, we document that the pool of newly synthesized precursor activator protein contains a majority of molecules with a complex-type oligosaccharide, which cannot contain a functional mannose-6-phosphate targeting signal. These molecules makeup the secreted forms of the protein in normal human fibroblasts. 相似文献
22.
The complex backpropagation algorithm 总被引:6,自引:0,他引:6
The backpropagation (BP) algorithm that provides a popular method for the design of a multilayer neural network to include complex coefficients and complex signals so that it can be applied to general radar signal processing and communications problems. It is shown that the network can classify complex signals. The generalization of the BP to deal with complex signals should make it possible to expand the line of applications of this powerful nonlinear signal processing algorithm 相似文献
23.
This paper presents an analysis of a monopole in free space. The result is given in a concise form that facilitates numerical programming. Moreover, the calculation involves no numerical integration and, thus, the computation is extremely fast 相似文献
24.
Abstract: Non-classical fractals generated by many generators are of interest. Random fractals caused by the random action of given generators and by random generrrtors are discussed The generators may have different scales. The fractal spectral function, the generalized fractal dimensions and scaling index are derived explicitly. Examples include the cracking patterns of concrete mix. 相似文献
25.
P M Reid A E Wilkinson K C Leung M N Jones 《Computer applications in the biosciences》1989,5(2):133-135
Analytical ultracentrifugation is commonly used for the determination of molecular weights (sedimentation equilibrium) and sedimentation coefficients (sedimentation rate) of biological macromolecules in solution. A Turbo Pascal program for the analysis of sedimentation equilibrium centrifugation data produced by absorbance optical systems is described. The user may enter data from a scan of absorbance versus distance from the centre of rotation, via a graphics tablet (or ASCII file). This is subsequently manipulated to yield an apparent weight average molecular weight for the given sample. Plots of ln (absorbance) versus (radius2) may also be produced. The method described uses readily available computational equipment requiring only a graphics tablet in addition to an IBM PC compatible computer. This technique and the software developed have been used to investigate the molecular weight range of two International Humic Substances Society (IHSS) reference samples from the Suwannee River. 相似文献
26.
An annular slot-coupled dielectric resonator antenna is investigated experimentally. As compared with the previous rectangular-slot version, the new configuration offers a much wider bandwidth of 18%. The return loss, radiation patterns, and antenna gain of the configuration have been measured and are discussed 相似文献
27.
A. Edwards Mulpuri V. Rao B. Molnar A. E. Wickenden W. Holland P. H. Chi 《Journal of Electronic Materials》1997,26(3):334-339
Doping by ion implantation using Si, O, Mg, and Ca has been studied in single crystal semi-insulating and n-type GaN grown
on a-sapphire substrates. The n-and p-type dopants used in this study are Si and O; Mg and Ca, respectively. Room temperature
activation of Si and O donors has been achieved after 1150°C annealing for 120 s. The activation of Mg and Ca acceptors is
too low to measure at both room temperature and 300°C. Using higher doses to achieve a measurable p-type conduction increases
the amount of damage created by the implantation. Rutherford back scattering measurements on this material indicate that the
damage is still present even after the maximum possible heat treatment. Secondary ion mass spectrometry measurements have
indicated a redistribution in the measured profiles of Mg due to annealing. 相似文献
28.
StructureandMagneticPropertiesofLaCo_(13-X)M_XIntermetallicCompoundsWUJianMin;LIFeng;TAILiChiandZHEMGQunStructureandMagneticP... 相似文献
29.
短毫米波和亚毫米波接收机的发展现状 总被引:1,自引:0,他引:1
本文概述了短毫米和亚毫米波接收机的发展现状,介绍了接收机的重要组成部件:混频器、振荡器和放大器的发展水平以及毫米波单片集成电路的现状,给出了接收机的最新性能指标。 相似文献
30.
EFFECTOFTHERATIOTh/UONTLDATINGACCURACY¥P.L.Leung(梁宝鎏);MichaelJ.Stokes(DepartmentofPhysicsandMaterialsScience,CityPolytechnico... 相似文献