全文获取类型
收费全文 | 1384篇 |
免费 | 72篇 |
国内免费 | 3篇 |
专业分类
电工技术 | 10篇 |
综合类 | 3篇 |
化学工业 | 402篇 |
金属工艺 | 22篇 |
机械仪表 | 19篇 |
建筑科学 | 73篇 |
能源动力 | 17篇 |
轻工业 | 210篇 |
水利工程 | 16篇 |
石油天然气 | 1篇 |
无线电 | 105篇 |
一般工业技术 | 221篇 |
冶金工业 | 195篇 |
原子能技术 | 10篇 |
自动化技术 | 155篇 |
出版年
2024年 | 5篇 |
2023年 | 25篇 |
2022年 | 68篇 |
2021年 | 87篇 |
2020年 | 53篇 |
2019年 | 33篇 |
2018年 | 44篇 |
2017年 | 36篇 |
2016年 | 45篇 |
2015年 | 48篇 |
2014年 | 44篇 |
2013年 | 82篇 |
2012年 | 102篇 |
2011年 | 101篇 |
2010年 | 75篇 |
2009年 | 82篇 |
2008年 | 104篇 |
2007年 | 68篇 |
2006年 | 49篇 |
2005年 | 41篇 |
2004年 | 35篇 |
2003年 | 36篇 |
2002年 | 24篇 |
2001年 | 24篇 |
2000年 | 12篇 |
1999年 | 20篇 |
1998年 | 12篇 |
1997年 | 12篇 |
1996年 | 14篇 |
1995年 | 8篇 |
1994年 | 8篇 |
1993年 | 7篇 |
1992年 | 7篇 |
1991年 | 1篇 |
1990年 | 4篇 |
1989年 | 6篇 |
1988年 | 1篇 |
1987年 | 11篇 |
1986年 | 5篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 6篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1976年 | 2篇 |
1968年 | 1篇 |
排序方式: 共有1459条查询结果,搜索用时 0 毫秒
51.
Chitosan-based fiber-sponge materials as a promising tool for microalgae harvesting from Lake Baikal
Timofei E. Grigoriev Ksenia I. Lukanina Pavel M. Gotovtsev Kirill V. Gorin Anna A. Melnikova Christina G. Antipova Roman Kamyshinsky Sergei N. Chvalun 《应用聚合物科学杂志》2020,137(40):49209
Mass eutrophication of microalgae and cyanobacteria is observed in Lake Baikal in the past decade. In this paper, the concept of replaceable adsorption filter material based on chitosan flocculant filler and chlorinated polyvinyl chloride polymer nonwoven material are proposed. Functional and mechanical properties and morphology of the material are investigated depending on a packing density and a degree of chitosan filling. The introduction of 45% chitosan increases the Young's modulus up to 10 times, and it makes the material more rigid in 2.8 times. The high efficiency of sorption and growth inhibition of cumulative biomass culture was shown. The biomass source is taken from the coast of Barguzinsky Bay of Lake Baikal. Dominant species is microalgae of Scenedesmus genus. 相似文献
52.
Christina Skiera Panagiotis Steliopoulos Thomas Kuballa Ulrike Holzgrabe Bernd Diehl 《Journal of the American Oil Chemists' Society》2012,89(8):1383-1391
Within the course of lipid peroxidation, hydroperoxides are formed as primary products. They can be used as analytical markers to assess the deterioration status of oils and fats. Here a new 1H-NMR assay to determine the hydroperoxide amount in edible oils is presented. We were able to show that the analytical performance of the method is similar to that of the commonly used peroxide value (PV) according to Wheeler. A total of 290 edible oil samples were analyzed using both methods. For some oil varieties considerable discrepancies were found between the results obtained. In the case of black seed and olive oil, two substances could be identified that cause positive (black seed oil) and negative (olive oils) deviations from the theoretical PV expected from the NMR values. 相似文献
53.
Jake E. Doiron Dr. Christina A. Le Dr. John Bacsa Prof. Gary W. Breton Prof. Kenneth L. Martin Prof. Stephen G. Aller Prof. Mark Turlington 《ChemMedChem》2020,15(18):1720-1730
Although the 1,2,3-triazole is a commonly used amide bioisostere in medicinal chemistry, the structural implications of this replacement have not been fully studied. Employing X-ray crystallography and computational studies, we report the spatial and electronic consequences of replacing an amide with the triazole in analogues of cystic fibrosis drugs in the VX-770 and VX-809 series. Crystallographic analyses quantify subtle differences in the relative positions and conformational preferences of the R1 and R2 substituents attached to the amide and triazole bioisosteres. Computational studies derived from the X-ray data highlight the improved hydrogen bonding donor and acceptor capabilities of the amide in comparison to the triazole. This analysis of the spatial and electronic differences between the amide and 1,2,3-triazole will inform medicinal chemists as they consider using the triazole as an amide bioisostere. 相似文献
54.
Agon Kokollari Marius Werner Christina Lindner Truc Lam Pham Prof. Dr. Franziska Thomas 《Chembiochem : a European journal of chemical biology》2023,24(22):e202300571
N-formylation is a common pre- and post-translational modification of the N-terminus or the lysine side chain of peptides and proteins that plays a role in the initiation of immune responses, gene expression, or epigenetics. Despite its high biological relevance, protocols for the chemical N-formylation of synthetic peptides are scarce. The few available methods are elaborate in their execution and the yields are highly sequence-dependent. We present a rapid, easy-to-use one-pot procedure that runs at room temperature and can be used to formylate protected peptides at both the N-terminus and the lysine side chain on the resin in near-quantitative yields. Only insensitive, storage-stable standard chemicals – formic acid, acetic anhydride, pyridine and DMF – are used. Formylation works for both short and long peptides of up to 34 amino acids and over the spectrum of canonical amino acids. 相似文献
55.
Mercer Kelly E.; Ahn Christina E.; Coke Amanda; Compadre Cesar M.; Drake Richard R. 《Protein engineering, design & selection : PEDS》2002,15(11):903-911
Understanding the functional and mechanistic properties of themulti-substrate herpes simplex virus type-1 thymidine kinase(HSV-1 TK) remains critical to defining its role as a majorpharmacological target in herpesvirus and gene therapies forcancer. An inherent limitation of the activity of HSV-TK isthe >70-fold difference in the Kms for phosphorylation ofthymidine over the pro-drug ganciclovir (GCV). To engineer anHSV-1 TK isoform that is specific for GCV as the preferred substrate,16 site-specific mutants were generated. The mutations wereconcentrated at conserved residues involved in nucleoside basebinding, Gln125 and near sites 3 and 4 involved in catalysisand substrate binding. The substrate preferences of each mutantenzyme were compared with wild-type HSV-1 TK. One mutant, termedQ7530 TK, had a lower Km for GCV than thymidine. Expressionof the Q7530 TK in tumor cells indicated comparable metabolismto and improved sensitivity to GCV over wild-type HSV-1 TK,with minimal thymidine phosphorylation activity. A molecularmodeling simulation of the different HSV-1 TK active-sites wasdone for GCV and thymidine binding. It was concluded that mutationsat Gln125 and near site 4, especially at Ala168, were responsiblefor loss of deoxypyrimidine substrate binding. 相似文献
56.
Sridhar Komarneni Bharat L. Newalkar Dongsheng Li Tarun Gheyi Christina L. Lopano Peter J. Heaney Jeffrey E. Post 《Journal of Porous Materials》2003,10(4):243-248
Several nitrate containing anionic clays were synthesized at different temperatures and the kinetics of NO3
– release were determined to test their suitability as slow-release N fertilizers. A sample (Mg:Al = 2:1) synthesized at 60°C with smaller particle size released 75, 86 and 100% of its NO3
– in 1, 3 and 7 days, respectively when equilibrated with a simulated soil solution. On the other hand, the 175°C/2 hrs sample with larger particle size released 65, 77 and 84% of its nitrate in 1, 3 and 7 days, respectively. Another anionic clay (synthesized at 175°C/24 hrs) of higher charge density (Mg:Al = 2:1) containing NO3
– was equilibrated with a 0.012 N NaCl or Na2CO3 to test the role of different anions in releasing the NO3
– anion from the interlayers. The results showed that Cl– released more NO3
– than did CO3
2– from this anionic clay after all the treatment times probably as a result of the CO3
2– anion blocking the release of NO3
– from the interior of the crystals. When a lower charge density (Mg:Al = 3:1) sample (synthesized at 175°C/48 hrs) was equilibrated with 0.02N solution of anions the release of nitrate was as follows: Cl– < F– < SO4
= CO3
2–. These results suggest that the divalent SO4
= and CO3
2– anions are more effective in the release of NO3
– from this lower charge density anionic clay. Time-resolved structural analysis of NO3
– exchange with CO3
2– in the above anionic clay using synchrotron x-ray diffraction showed that ion exchange is rapid because of small crystal size and lower charge density. Thus the release of NO3
– from anionic clays is an interplay among the type of anions present in soil solution, their concentration, pH of soil solution, the charge density and crystal size of anionic clay etc. 相似文献
57.
Anna Schüller Daniel Matzner Dr. Christina E. Lünse Prof. Dr. Valentin Wittmann Dr. Catherine Schumacher Sandra Unsleber Prof. Dr. Heike Brötz‐Oesterhelt Prof. Dr. Christoph Mayer Prof. Dr. Gabriele Bierbaum Prof. Dr. Günter Mayer 《Chembiochem : a European journal of chemical biology》2017,18(5):435-440
The ever‐growing number of pathogenic bacteria resistant to treatment with antibiotics call for the development of novel compounds with as‐yet unexplored modes of action. Here, we demonstrate the in vivo antibacterial activity of carba‐α‐d ‐glucosamine (CGlcN). In this mode of action study, we provide evidence that CGlcN‐mediated growth inhibition is due to glmS ribozyme activation, and we demonstrate that CGlcN hijacks an endogenous activation pathway, hence utilizing a prodrug mechanism. This is the first report describing antibacterial activity mediated by activating the self‐cleaving properties of a ribozyme. Our results open the path towards a compound class with an entirely novel and distinct molecular mechanism. 相似文献
58.
Dr. Christina Haußner Dominik Damm Sandra Nirschl Anette Rohrhofer Prof. Dr. Barbara Schmidt Prof. Dr. Jutta Eichler 《Chembiochem : a European journal of chemical biology》2017,18(7):647-653
The broadly neutralizing HIV‐1 antibody b12 recognizes the CD4 binding site of the HIV‐1 envelope glycoprotein gp120 and efficiently neutralizes HIV‐1 infections in vitro and in vivo. Based on the 3D structure of a b12 ? gp120 complex, we have designed an assembled peptide (b12‐M) that presents the parts of the three heavy‐chain complementarity‐determining regions (CDRs) of b12, which contain the contact sites of the antibody for gp120. This b12‐mimetic peptide, as well as a truncated peptide presenting only two of the three heavy‐chain CDRs of b12, were shown to recognize gp120 in a similar manner to b12, as well as to inhibit HIV‐1 infection, demonstrating functional mimicry of b12 by the paratope mimetic peptides. 相似文献
59.
Etya Amsalem Mario Padilla Paul M. Schreiber Naomi S. Altman Abraham Hefetz Christina M. Grozinger 《Journal of chemical ecology》2017,43(6):563-572
Reproduction in social insect societies reflects a delicate balance between cooperation and conflict over offspring production, and worker reproduction is widespread even in species showing strong reproductive skew in favor of the queen. To navigate these conflicts, workers are predicted to develop the means to estimate the queen’s fecundity - potentially through behavioral and/or chemical cues - and to adjust their reproduction to maximize their fitness. Here, we introduced bumble bee, Bombus impatiens, workers to queens of different mating and reproductive status and examined worker reproduction and expression levels of two genes which were previously shown to be sensitive to the presence of the queen, vitellogenin and Krüppel-homolog 1. We further explored whether the queen’s chemical secretion alone is sufficient to regulate worker reproduction, aggression and gene expression. We found that worker ovary activation was inhibited only in the presence of egg-laying queens, regardless of their mating status. Workers reared in the presence of newly-mated queens showed intermediate vitellogenin expression levels relative to workers reared with mated egg-laying and virgin queens. However, none of the whole-body chemical extracts of any of the queen treatment groups affected ovary activation, aggressive behavior, or gene expression in workers. Our findings indicate that only the presence of a freely-behaving, egg-laying queen can fully inhibit worker reproduction. It remains to be determined if workers detect differences in queen mating status and fecundity through differences in the queens’ behavior alone or through the queen’s behavior in concert with fertility signals. 相似文献
60.
Ganoderma is normally dried to extend its shelf life without using chemical preservative and to concentrate the medicinal value in the fruiting body. Convective hot air drying characteristics of Ganoderma tsugae Murrill were evaluated in hot air circulated oven at different drying temperatures, sizes, and air flow rates. The drying kinetics of Ganoderma tsugae in kidney shape and slices were investigated and compared at different drying conditions. The variation of effective moisture diffusivity values at decreasing moisture contents during drying was determined from the drying data. Four well-known thin-layer drying models were fitted to the experimental data and the Midilli model was found to satisfactory describe the drying characteristics of kidney-shaped Ganoderma tsugae. Ganoderma tsugae dried at 50°C with air velocity of 1.401 ms?1 showed the highest retention of crude ganoderic acid content compared to other drying conditions. 相似文献