Kell and Kx are two quantitatively minor proteins from the human erythrocyte membrane which carry blood groups antigens and are thought to be a metalloprotease and a membrane transporter, respectively. In the red cell membrane, these proteins form a complex stabilized by disulfide bond(s). Phosphorylation status of these proteins was studied, in the presence or absence of effectors of several kinases, either on intact cells incubated with [32P]-orthophosphate or on ghosts incubated with [gamma-32P]ATP. Purification of Kell-Kx complex, by immunochromatography on an immobilized human monoclonal antibody of Kell blood group specificity allowed to establish that (i) neither protein is phosphorylated on tyrosine; (ii) the Kell protein is a putative substrate for Casein Kinase II (CKII) and Casein Kinase I (CKI) but not for protein kinase C (PKC), whereas Kx protein is phosphorylated by CKII and PKC but not by CKI; (iii) Protein Kinase A neither phosphorylates the Kell nor the Kx proteins. 相似文献
Recently many runoff models based on cellular automaton (CA) have been developed to simulate floods; however, the existing models cannot be readily applied to complex urban environments. This study proposes a novel rainfall-runoff model based on CA (RRCA) to simulate inundation. Its main contributions include a fine runoff generation process that considers 12 urban scenarios rather than a single land use type and the confluence process determined by the new transition rules considering water supply and demand (WS-WD transition rules). RRCA was compared with another CA based flood model (E2DCA). With the benchmark model, the results showed that there was good agreement, with an R-squared greater than 0.9, and that RRCA was more sensitive to waterlogging levels than E2DCA. Furthermore, the simulated vegetation interception, infiltration and drainage processes had varying degrees of impact on waterlogging. Corresponding measures can be taken in urban flood management according to the identification of areas experiencing drainage difficulties.
The Journal of Supercomputing - Sentiment analysis in Danmaku video interaction aims at measuring public mood in respect of the video, which is helpful for the potential applications in behavioral... 相似文献
Neural Computing and Applications - RNA-binding proteins play an important role in the biological process. However, the traditional experiment technology to predict RNA-binding residues is... 相似文献
The rapid growth of motion capture data increases the importance of motion retrieval. The majority of the existing motion retrieval approaches are based on a labor-intensive step in which the user browses and selects a desired query motion clip from the large motion clip database. In this work, a novel sketching interface for defining the query is presented. This simple approach allows users to define the required motion by sketching several motion strokes over a drawn character, which requires less effort and extends the users’ expressiveness. To support the real-time interface, a specialized encoding of the motions and the hand-drawn query is required. Here, we introduce a novel hierarchical encoding scheme based on a set of orthonormal spherical harmonic (SH) basis functions, which provides a compact representation, and avoids the CPU/processing intensive stage of temporal alignment used by previous solutions. Experimental results show that the proposed approach can well retrieve the motions, and is capable of retrieve logically and numerically similar motions, which is superior to previous approaches. The user study shows that the proposed system can be a useful tool to input motion query if the users are familiar with it. Finally, an application of generating a 3D animation from a hand-drawn comics strip is demonstrated. 相似文献