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991.
Base excision repair (BER) constitutes a ubiquitous excision repair mechanism, which is responsible for the removal of multiple types of damaged and inappropriate bases in DNA. We have employed a yeast cell-free system to examine the biochemical mechanism of the BER pathway in lower eukaryotes. Using uracil-containing DNA as a model substrate, we demonstrate that yeast BER requires Apn1 protein, an Escherichia coli endonuclease IV homolog. In extracts of an apn1 deletion mutant, the 5'-incision at AP (apurinic/apyrimidinic) sites is not detectable, supporting the notion that yeast contains only one major 5'-AP endonuclease. The processing of the 5'-deoxyribose phosphate moieties was found to be a rate-limiting step. During BER of uracil-containing DNA, repair patch sizes of 1-5 nucleotides were detected, with single nucleotide repair patches predominant. 相似文献
992.
H9000系统在西北电网水调自动化系统工程中的应用 总被引:2,自引:0,他引:2
西北电网水调自动化系统主要用于自动采集管理西北电网分布在三大水系的8个水电厂、相关流域内100个水文站、200个雨量站的实时信息,以便实现水库群跨流域补偿,统一协调发电与防洪、排沙、防凌、灌溉等一系列矛盾,解决水库群综合利用问题.系统主要具有上。下特点:(1)开放的系统环境;(2)高可乘性;(3)多样化的数据规约;(4)开放的人机联系手段;(5)务性能的数据库;(6)丰富的水调应用软件.其中水调应用软件包括:(1)汉江上游石泉、安康梯级联合洪水预报;(2)黄河上游龙羊峡、李家峡、刘家峡两库三站短期(日)调度;(3)水务综合统计分析软件.西北水调自动化系统将使H9000系统在大规模网络关系数据库管理技术、高级图形处理技术、复杂网络组网技术、容措技术、水调高级应用软件技术等方面提高到一个更高的水平. 相似文献
993.
Previous film autoradiographic studies demonstrated that, during corticogenesis, dopamine receptors of the D1 class are abundant in the embryonic primate cerebral wall. In the present study, we expand these findings by identifying the cellular elements of the fetal occipital cerebral wall expressing D1 and D5 subtypes of the D1 dopamine receptor class. We have examined tissue from monkey fetuses collected at 70, 90 and 120 days of gestation using antibodies directed against C-termini of the D1 and D5 dopamine receptors. At all three embryonic ages studied, we found D1 and D5 receptors expressed by multiple cell types of the embryonic cerebral wall. Both D1 and D5 receptor proteins are produced by pyramidal neurons of the cortical plate and by a variety of interstitial neurons of the subplate and intermediate zones. D1 and D5 receptors are also present in cells of the proliferative ventricular and subventricular zones, some of which were identified as dividing cells. In addition, D1 and D5 receptors are detectable in the protoplasmic astroglial and ependymal cells distinguishable in monkey fetuses collected at 120 days of gestation. Some cellular elements of the embryonic monkey cerebral wall express only one subtype of the D1 dopamine receptor class. For example, embryonic Cajal-Retzius neurons in the marginal zone and migrating neurons in the intermediate zone are immunoreactive only to D5 antisera. In contrast, radial glia can be labeled only with D1 receptor-specific antisera. Finally, only D1 receptors are detectable in the blood vessels penetrating the embryonic monkey cerebral wall. Based on these observations, we propose that dopamine receptors of the D1 class play an important role in regulating cerebral cortical formation and that D1 and D5 receptor subtypes may participate in regulation of different aspects of this process. 相似文献
994.
多灰度级显示器上的曲线绘制 总被引:1,自引:0,他引:1
光栅扫描显示器的屏幕是由位置固定的,离散的象素点所组成的,因而在其上生成的曲线只能是实际曲线的某种近似表示,并不是很美观的,例如有曲线的阶梯效应及曲线亮度不均度等问题,该文说明利用多灰度光栅显露器可以在一定程度上解决这些问题,并且用给出的主些算法绘制曲线时,可以较好地解决上述问题,这些算法在动画制作中也可有典型的应用。 相似文献
995.
A deterministic digital simulation model with finite sum of properly weighted sinusoids is used to simulate a frequency nonselective mobile channel with LOS component. Measurement data are used to optimise the parameters. Which are then used in the deterministic simulation model. The probability distribution and level cross rate (LCR) obtained from the measured data agree well with the analytical and simulation results 相似文献
996.
The narrow pore-size distribution of activated carbon fibres (ACF) limits their application in the fields concerning larger
molecules, such as liquid adsorption and catalyst support. On the addition of carbon particles and organic materials to polyacrylonitrile
fibres, and after stabilizing and activating, the mesoporosity in the resultant ACF has been obviously increased. Among these
additives, carbon black gives the best effect. With 1 wt% carbon black I in precursor and on activating at 880°C for 30 min,
a mesoporosity of 48.2% (total pore volume 0.704 ml g-1, mesopore volume 0.340 ml g-1) has been obtained. The mechanism of
mesopore formation is also discussed.
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
997.
Design of finite-length metal-clad optical waveguide polarizer 总被引:1,自引:0,他引:1
Chyong-Hua Chen Likarn Wang 《Quantum Electronics, IEEE Journal of》1998,34(7):1089-1097
The performance of optical metal-clad waveguide polarizers is analyzed in this paper with a view to optimizing the extinction ratio, a condition for which power of the TM mode is completely attenuated is identified here. In general, such a condition corresponding to an infinitely high extinction ratio can be met for a wide range of buffer layer thicknesses by selection of metal film thickness and polarizer length. When a very thick (e.g., semi-infinitely thick) metal film is used, the aforementioned condition can be met with a properly chosen buffer layer thickness and polarizer length. The numerical results show that all the polarizers designed here for realization of infinitely high extinction ratios have either quite low or reasonably acceptable attenuations for the TE mode 相似文献
998.
In this paper we propose a multiobjective decision making based neural-network model and algorithm for image reconstruction from projections. This model combines the Hopfield's model and multiobjective decision making approach. We develop a weighted sum optimization based neural-network algorithm. The dynamical process of the net is based on minimization of a weighted sum energy function and Euler's iteration, and apply this algorithm to image reconstruction from computer-generated noisy projections and Siemens Somatson DR scanner data, respectively. Reconstructions based on this method is shown to be superior to conventional iterative reconstruction algorithms such as the multiplicate algebraic reconstruction technique (MART) and convolution from the point of view of accuracy of reconstruction. Computer simulation using the multiobjective method shows a significant improvement in image quality and convergence behavior over the conventional algorithms 相似文献
999.
Wang Menghua 《中国皮革》1998,(9)
本文讨论了在黄牛鞋面革生产过程中产生松面的原因,并经过多年的生产实践,摸索出解决松面问题行之有效的技术措施。 相似文献
1000.
Myostatin (GDF-8) is a member of the transforming growth factor-beta superfamily and plays a role in muscle growth and development. Mice having targeted disruption of this gene display marked increases in muscle mass, a phenotype similar to the muscular hypertrophy (mh) in several cattle breeds. Physical mapping data developed from YAC clones indicate the bovine myostatin gene lies close to the centromere of bovine Chromosome (Chr) 2 (BTA2) at 2q11, indistinguishable from the cytogenetic location of the mh locus. In addition, a polymorphism in the second intron of the gene was used to show that myostatin maps within the interval previously shown to contain mh. These data suggest myostatin may be the gene causing muscular hypertrophy in cattle. 相似文献