Application of HUMF13A01 (AAAG)n STR polymorphism to the genetic diagnosis of coagulation factor XIII deficiency

Deficiency of the A subunit of coagulation factor XIII causes a severe bleeding disorder requiring life long replacement therapy. The mutations causing A subunit deficiency appear to be very heterogeneous, and it is impractical to identify each mutation before genetic counselling or prenatal diagnosis can be attempted. In this study we have shown that a highly polymorphic short tandem repeat element, HUMF13A01 (AAAG)n that occurs in the 5' flanking sequence of the factor XIII A subunit gene, can be used to follow the segregation of deficiency causing mutations. We studied 6 families with factor XIII A subunit deficiency from 5 different ethnic groups. All parents were heterozygous for the repetitive element and therefore all the families were informative. The linked polymorphism was used to carry out the first prenatal diagnosis of factor XIII A subunit deficiency. The analysis of this polymorphism by the polymerase chain reaction is rapid, reliable, requires little DNA and is ideal for the genetic analysis of factor XIII A subunit deficiency.     

Three-dimensional electromagnetic cross-well inversion

An inversion algorithm for a vertical magnetic dipole source and a vertical magnetic field component receiver is presented. A three-dimensional integral equation algorithm is used for calculating the electromagnetic response of a particular trial reservoir model. The inversion formalism used is the Marquardt technique of nonlinear least-squares optimization. The system derivatives are calculated using an exact expression derived from reciprocity. The derivative calculation involves introducing sources at the receiver locations with subsequent back-substitution into the impedance matrix equation. The inversion algorithm was tested on data gathered with a laboratory scale model. Convergence to the neighborhood of the correct model from distant initial trial models is good     

An OFDM scheme with a half complexity

The paper deals with an OFDM (orthogonal frequency division multiplexing) system based on filter-bank architecture. The known implementation uses a DFT (discrete Fourier transform) processor and a polyphase network (PPN). Even if it is based on complex components, in the final step it operates the real part extraction of the incoming signal. This leads to redundant operations in the DFT processor and in the PPN. Specifically, for the transmission of N complex symbol sequences at a given rate ½F, an N-point DFT processor and an N-branch PPN, both working at the rate F, are required. This implementation can be improved with a complexity reduction by a factor of two. In fact, in the paper an architecture is presented based on an N-point DFT processor and N-branch PPN at the rate F, for the transmission of 2N (in place of N) complex symbol sequences at the rate ½F     

A stochastic deconvolution method to reconstruct insulin secretionrate after a glucose stimulus

Insulin secretion rate (ISR) is not directly measurable in man but it can be reconstructed from C-peptide (CP) concentration measurements by solving an input estimation problem by deconvolution. The major difficulties posed by the estimation of ISR after a glucose stimulus, e.g., during an intravenous glucose tolerance test (IVGTT), are the ill-conditioning of the problem, the nonstationary pattern of the secretion rate, and the nonuniform/infrequent sampling schedule. In this work, a nonparametric method based on the classic Phillips-Tikhonov regularization approach is presented. The problem of nonuniform/infrequent sampling is addressed by a novel formulation of the regularization method which allows the estimation of quasi time-continuous input profiles. The input estimation problem is stated into a Bayesian context, where the a priori known nonstationary characteristics of ISR after the glucose stimulus are described by a stochastic model. Deconvolution is tackled by linear minimum variance estimation, thus allowing the derivation of new statistically based regularization criteria. Finally, a Monte-Carlo strategy is implemented to assess the uncertainty of the estimated ISR arising from CP measurement error and impulse response parameters uncertainty     

Zirconium recovery from zircaloy shavings

A chlorination process for recovering Zr from zircaloy scrap has been studied. Zircaloy chlorination was possible at temperatures as low as 220 °C. The scale microstructure and its effect on the zircaloy reactivity was analysed using Thermogravimetric analysis (TGA), X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDXS) and scanning electron microscopy (SEM) techniques. A solid-solid phase transformation took place into the oxide scale during the zircaloy chlorination. Zirconium, as ZrCl₄(g), was separated from the oxide scale and chlorides of Cr and Fe. The effect of the reaction temperature was also analysed.     

Vessel wall detection and blood noise reduction in intravascularultrasound imaging

Scattering from blood limits the contrast between the vessel wall and the lumen in intravascular ultrasound imaging. This makes it difficult to localize the vessel wall, especially on still images. This paper presents a method for automatic detection of vessel walls and reduction of blood noise based on correlation of the RF-signal between adjacent frames. The ultrasound RF-signal is quadrature demodulated, digitized, stored in memory, and transferred to a computer for processing and analysis. The absolute value of the cross-correlation coefficient between two adjacent frames is used to differentiate between stationary and fluctuating signals. Models and numerical calculations presented in this work indicate that the cross-correlation coefficient obtained from a radially dilating vessel wall will be larger than 0.8 under standard 20 MHz imaging conditions. The corresponding value from blood is less than 0.2 for blood velocities exceeding 0.5 cm s^-1 . The blood-noise filter is based on detecting this difference in correlation and displays vessel wall regions with no modifications, while regions detected as blood are rejected. A simplified vessel-wall detector that is suitable for real-time implementation is proposed. The performance of this detector and the blood noise filter are demonstrated by in vitro experiments     

Evaluating design metrics on large-scale software

Design-quality metrics that are being developed for predicting the potential quality and complexity of the system being developed are discussed. The goal of the metrics is to provide timely design information that can guide a developer in producing a higher quality product. The metrics were tested on projects developed in a university setting with client partners in industry. To further validate the metrics, they were applied to professionally developed systems to test their predictive qualities. The results of applying two design metrics and a composite metric to data from a large-scale industrial project are presented     

A dislocation shielding prediction of the toughness transition during cleavage of semibrittle crystals

An interconnected set of observations assesses current equilibrium models of the ductile-brittle-transition temperature (DBTT). This involvesin situ transmission electron microscopy (TEM) studies of crack-tip dislocations in single and polycrystals and bulk fracture toughness tests at various temperatures. Beyond K_I values of 8 MPa · m^1/2 in both iron-base single and polycrystals, large numbers of redundant dislocations are created, as postulated recently by Weertman. [38] Still, the necessary shielding dislocations, as required by equilibrium, can be detected at values as high as 20 and 40 MPa · m^1/2 byex situ TEM and electron channeling, respectively. In addition, the close approach of dislocations to the crack tip in some of the studies, as opposed to others, suggests that large dislocation free zones (DFZ) are a thin-film artifact. However, a failure criterion based partly on the Rice-Thomson model’21 is both consistent with the absence of a large DFZ and observed fracture toughness variations with test temperature. It is emphasized that this toughness transition is entirely in the semibrittle regime where cleavage is the failure mode. Nevertheless,K _lc values increase from 3 to 60 MPa·m^1/2 with an increase in test temperature. This article is based on a presentation made in the symposium “Quasi-Brittle Fracture” presented during the TMS fall meeting, Cincinnati, OH, October 21–24, 1991, under the auspices of the TMS Mechanical Metallurgy Committee and the ASM/MSD Flow and Fracture Committee.