Development of Anticandidal Delivery Systems: (I) Anticandidal Activities of Antifungal Agents and Synergistic Combination with Other Drugs

Susceptibility testing with antifungal agents, e. g., minimum inhibitory concentration (MIC) determination, is performed to obtain reliable data that permit selection of the most suitable agents for treatment of an infective condition. To determine the drugs that provide maximum effectiveness against oral candidiasis, the MICs of various antifungal agents were determined. Also, synergism between two chosen antifungal agents was evaluated, and the effect of benzocaine, an anesthetic, and hydrocortisone, an antiinflammatory agent, on their MICs was examined. It was observed that among all the drugs tested, clotrimazole was the most promising candidate for use as an oral local antifungal. The combination of clotrimazole and chlorhexidine resulted in a decrease in the MIC. While the addition of hydrocortisone to this combination resulted in a slight increase in the MIC, the inclusion of benzocaine resulted in a substantial decrease in the MIC of the antifungal agent combination.     

Adhesion, growth and differentiation of human bone marrow stromal cells on non-porous calcium carbonate and plastic substrata: effects of dexamethasone and 1,25dihydroxyvitamin D3

Bone marrow contains stromal fibroblastic stem cells which have the potential to differentiate into bone-forming cells. Therefore addition of bone marrow to porous bone substitutes such as coral or coralline hydroxyapatite may be expected to enhance bone ingrowth into these implants. This study was designed to evaluate the possibility of growing human bone marrow stromal fibroblastic cells (HBMC) on a calcium carbonate substrate. For this purpose, HBMC were cultured for 20 days on plastic or calcium carbonate and cell adhesion, growth, and differentiation were studied. It was concluded that calcium carbonate is a highly compatible material for the growth of HBMC. Cells were capable of adhesion within 30 min and were spread within 24 h on this material. However, plating efficiency was decreased in comparison to plastic. Population doubling times (PDT) showed that they were similar when the cells were grown on plastic or calcium carbonate as substratum (PDT=4, 5.5 days). Early protein synthesis included collagen I, collagen III, osteopontin and bone sialoprotein. To induce differentiation of HBMC on plastic and calcium carbonate the influence of dexamethasone (Dex) and 1,25dihydroxyvitamin D3 (1,25(OH)₂D₃) on alkaline phosphatase (ALP) expression was studied. Basic ALP activity was similar when cells were grown on plastic or calcium carbonate. However, Dex and 1,25(OH)₂D₃ increased ALP activity of HBMC which could be driven best towards osteogenesis in the presence of Dex and 1,25(OH)₂D₃.     

Adaptive solutions to the mutual exclusion problem

Summary Algorithms for mutual exclusion that adapt to the current degree of contention are developed. Afilter and a leader election algorithm form the basic building blocks. The algorithms achieve system response times that are independent of the total number of processes and governed instead by the current degree of contention. The final algorithm achieves a constant amortized system response time. Manhoi Choy was born in 1967 in Hong Kong. He received his B.Sc. in Electrical and Electronic Engineerings from the University of Hong Kong in 1989, and his M.Sc. in Computer Science from the University of California at Santa Barbara in 1991. Currently, he is working on his Ph.D. in Computer Science at the University of California at Santa Barbara. His research interests are in the areas of parallel and distributed systems, and distributed algorithms. Ambuj K. Singh is an Assistant Professor in the Department of Computer Science at the University of California, Santa Barbara. He received a Ph.D. in Computer Science from the University of Texas at Austin in 1989, an M.S. in Computer Science from Iowa State University in 1984, and a B.Tech. from the Indian Institute of Technology at Kharagpur in 1982. His research interests are in the areas of adaptive resource allocation, concurrent program development, and distributed shared memory.A preliminary version of the paper appeared in the 12th Annual ACM Symposium on Principles of Distributed ComputingWork supported in part by NSF grants CCR-9008628 and CCR-9223094     

GIBC formulation for the resonant frequencies and fielddistribution of a substrate-mounted dielectric resonator

Dielectric resonators (DR's) are widely used in telecommunication systems. A method is proposed here to find the resonant frequency and field distribution in a substrate-mounted DR structure. The field of a dielectric rod has been decomposed into a combination of guided modes with unknown coefficients and an unknown continuous spectrum of radiation field. The unknowns are then obtained by applying two generalized impedance boundary conditions (GIBC's) representing the substrate and air layers at the top and bottom of the DR. This leads to the calculation of the total (guided+radiation) field as well as the resonant frequency of the structure     

Prediction of thermal expansion coefficients of plain weave fabric composites

Three plain weave fabric composite analysis models are presented for the prediction of the on-axes thermal expansion coefficients. These are two-dimensional models in the sense that the actual strand cross-sectional geometry, strand undulation and the presence of a gap between the adjacent strands are taken into account. In the first two models, termed refined models, the representative unit cell is discretised into slices and elements and analysed. In the third method, a closed-form solution is presented. In this case, the representative unit cell is idealised as a crossply laminate and analysed. The relative merits and demerits of the models are also discussed. The predicted results are compared with the experimental values. A good correlation is observed.     

Advances in speech transcription at IBM under the DARPA EARS program

This paper describes the technical and system building advances made in IBM's speech recognition technology over the course of the Defense Advanced Research Projects Agency (DARPA) Effective Affordable Reusable Speech-to-Text (EARS) program. At a technical level, these advances include the development of a new form of feature-based minimum phone error training (fMPE), the use of large-scale discriminatively trained full-covariance Gaussian models, the use of septaphone acoustic context in static decoding graphs, and improvements in basic decoding algorithms. At a system building level, the advances include a system architecture based on cross-adaptation and the incorporation of 2100 h of training data in every system component. We present results on English conversational telephony test data from the 2003 and 2004 NIST evaluations. The combination of technical advances and an order of magnitude more training data in 2004 reduced the error rate on the 2003 test set by approximately 21% relative-from 20.4% to 16.1%-over the most accurate system in the 2003 evaluation and produced the most accurate results on the 2004 test sets in every speed category.     

Syntax and consistent equation semantics of hybrid Chi

The hybrid χ (Chi) formalism integrates concepts from dynamics and control theory with concepts from computer science, in particular from process algebra and hybrid automata. It integrates ease of modeling with a straightforward, structured operational semantics. Its ‘consistent equation semantics’ enforces state changes to be consistent with delay predicates, that combine the invariant and flow clauses of hybrid automata. Ease of modeling is ensured by means of the following concepts: (1) different classes of variables: discrete and continuous, of subclass jumping or non-jumping, and algebraic; (2) strong time determinism of alternative composition in combination with delayable guards; (3) integration of urgent and non-urgent actions; (4) differential algebraic equations as a process term as in mathematics; (5) steady-state initialization; and 6) several user-friendly syntactic extensions. Furthermore, the χ formalism incorporates several concepts for complex system specification: (1) process terms for scoping that integrate abstraction, local variables, local channels and local recursion definitions; (2) process definition and instantiation that enable process re-use, encapsulation, hierarchical and/or modular composition of processes; and (3) different interaction mechanisms: handshake synchronization and synchronous communication that allow interaction between processes without sharing variables, and shared variables that enable modular composition of continuous-time or hybrid processes. The syntax and semantics are illustrated using several examples.     

Adaptive Spatial Partitioning for Multidimensional Data Streams

We propose a space-efficient scheme for summarizing multidimensional data streams. Our sketch can be used to solve spatial versions of several classical data stream queries efficiently. For instance, we can track ε-hot spots, which are congruent boxes containing at least an ε fraction of the stream, and maintain hierarchical heavy hitters in d dimensions. Our sketch can also be viewed as a multidimensional generalization of the ε-approximate quantile summary. The space complexity of our scheme is O((1/ε) log R) if the points lie in the domain [0, R]^d, where d is assumed to be a constant. The scheme extends to the sliding window model with a log (ε n) factor increase in space, where n is the size of the sliding window. Our sketch can also be used to answer ε-approximate rectangular range queries over a stream of d-dimensional points.