国外如何打造节能住宅

有关国内专家在对英国、德国、挪威、瑞典、荷兰等欧洲国家进行学习考察后发现，单在多、高层住宅的形体高度、外观造型和立面色彩方面，国内与欧洲国家已看不出有多大的区别，国内许多著名小区的住宅放到欧洲去也称得上是上乘之作。但在节能等内在品质上，差距非常之大。[编者按]     

Effects of dissolved organic matter from the rhizosphere of the hyperaccumulator Sedum alfredii on sorption of zinc and cadmium by different soils

Pot experiments were conducted to investigate the changes of the dissolved organic matter (DOM) in the rhizosphere of hyperaccumulating ecotype (HE) and non-hyperaccumulating ecotype (NHE) of Sedum alfredii and its effects on Zn and Cd sorption by soils. After planted with HE, soil pH in the rhizosphere reduced by 0.5-0.6 units which is consistent with the increase of DOM. The hydrophilic fractions (51%) in DOM from the rhizosphere of HE (HE-DOM) was much greater than NHE-DOM (35%). In the presence of HE-DOM, Zn and Cd sorption capacity decreased markedly in the following order: calcareous clay loam > neutral clay loam > acidic silty clay. The sorption isotherms could be well described by the Freundlich equation (R² > 0.95), and the partition coefficient (K) in the presence of HE-DOM was decreased by 30.7-68.8% for Zn and 20.3-59.2% for Cd, as compared to NHE-DOM. An increase in HE-DOM concentration significantly reduced the sorption and increased the desorption of Zn and Cd by three soils. DOM derived from the rhizosphere of the hyperaccumulating ecotype of S. alfredii could significantly reduce metal sorption and increase its mobility through the formation of soluble DOM-metal complexes.     

Glucagon-like peptide-2 (GLP-2) increases small intestinal blood flow and mucosal growth in ruminating calves

Glucagon-like peptide-2 (GLP-2) increases small intestinal mass and blood flow in nonruminants but its effect in ruminants is unknown. Eight Holstein calves with an ultrasonic flow probe around the superior mesenteric artery and catheters in the carotid artery and mesenteric vein were paired by age and randomly assigned to treatment of a control (0.5% of BSA in saline; n = 4) or GLP-2 (50 μg/kg of body weight of bovine GLP-2 in BSA; n = 4) given subcutaneously every 12 h for 10 d. Blood flow was measured on d 0 (acute) and d 10 (chronic) and included 3 periods: baseline (saline infusion), treatment (infusion of BSA or 1,000 pmol of GLP-2/kg of body weight per h), and recovery (saline infusion). On d 11, calves were killed 2 h after injection of 5-bromo-2′-deoxyuridine (BrdU). Gastrointestinal tissues were weighed and epithelial samples were obtained to determine villus height, crypt depth, and BrdU staining. Infusion of GLP-2 increased superior mesenteric artery blood flow to 175% of baseline on d 0 but to only 137% of baseline after chronic treatment. Compared with that of the control, GLP-2 increased small intestinal mass by 24% by increasing epithelial mass in the jejunum and ileum. Additionally, GLP-2 increased villus height, crypt depth, and BrdU-labeling in small intestinal segments. These results demonstrate that GLP-2 induces similar increases in small intestinal blood flow and growth in ruminants to those observed in nonruminants. Furthermore, GLP-2 increases small intestinal blood flow in ruminants but this response is attenuated after 10 d of GLP-2 administration. In cattle, GLP-2 may be an important hormone in the regulation of intestinal blood flow and epithelial growth.     

Engineered Amp C β-lactamase as a fluorescent screening tool for class C β-lactamase inhibitors

Class C β-lactamases mediate antibiotic resistance in bacteria by efficiently hydrolyzing a broad range of β-lactam antibiotics. With their clinical significance and the lack of commercially available effective inhibitors, development of class C β-lactamase inhibitors has become one of the recent hot issues in the pharmaceutical industry. In this paper, we report the protein engineering of a fluorescent Amp C β-lactamase mutant designated as V211Cf for the in vitro screening of class C β-lactamase inhibitors. When a fluorescein (f) was incorporated at the entrance of the enzyme's active site (position 211), Amp C β-lactamase from Enterobacter cloacae P99 was tailor-made into a novel fluorescent biosensing protein that could display a fluorescence enhancement upon binding with its β-lactam substrates/inhibitors. With its catalytic activity close to the wild-type level, V211Cf can act as a "natural" fluorescent drug target for screening small binding molecules. In addition, V211Cf can allow specific detection for its active-site binding molecules and discriminate them from nondruglike molecules in the screen. Furthermore, V211Cf is amenable to a high throughput format. Taken together, V211Cf demonstrates the potential as an efficient tool for screening class C β-lactamase inhibitors and facilitates the discovery of therapeutics that can combat the clinically important class C β-lactamases.     

高阻硅作衬底的短沟p—MOS晶体管

文章从定性方面研究了在高阻硅(3～10Ω·cm)衬底上,用3μmCMOS工艺制作的短沟(2.5～25μm)pMOS晶体管的性能,特别是这些器件用于模拟场合时在本体(|V_(gs)|>|V_r|)下的噪声性能。两种模式的噪声性能都用1/f噪声(谱频达约100Hz)和由衬底决定的白噪声来表征。研究了它们在导通区和关断区的性能。模拟和实验数据表明,这些晶体管具有二次线性区(称之为准线性区)而不是通常理论所描述的饱和区。