Validation of a Cleanroom Compliant Sonication-Based Decellularization Technique: A New Concept in Nerve Allograft Production

Defects of the peripheral nervous system are extremely frequent in trauma and surgeries and have high socioeconomic costs. If the direct suture of a lesion is not possible, i.e., nerve gap > 2 cm, it is necessary to use grafts. While the gold standard is the autograft, it has disadvantages related to its harvesting, with an inevitable functional deficit and further morbidity. An alternative to autografting is represented by the acellular nerve allograft (ANA), which avoids disadvantages of autograft harvesting and fresh allograft rejection. In this research, the authors intend to transfer to human nerves a novel technique, previously implemented in animal models, to decellularize nerves. The new method is based on soaking the nerve tissues in decellularizing solutions while associating ultrasounds and freeze–thaw cycles. It is performed without interrupting the sterility chain, so that the new graft may not require post-production γ-ray irradiation, which is suspected to affect the structural and functional quality of tissues. The new method is rapid, safe, and inexpensive if compared with available commercial ANAs. Histology and immunohistochemistry have been adopted to evaluate the new decellularized nerves. The study shows that the new method can be applied to human nerve samples, obtaining similar, and, sometimes better, results compared with the chosen control method, the Hudson technique.     

Vandetanib versus Cabozantinib in Medullary Thyroid Carcinoma: A Focus on Anti-Angiogenic Effects in Zebrafish Model

Medullary thyroid carcinoma (MTC) is a tumor deriving from the thyroid C cells. Vandetanib (VAN) and cabozantinib (CAB) are two tyrosine kinase inhibitors targeting REarranged during Transfection (RET) and other kinase receptors and are approved for the treatment of advanced MTC. We aim to compare the in vitro and in vivo anti-tumor activity of VAN and CAB in MTC. The effects of VAN and CAB on viability, cell cycle, and apoptosis of TT and MZ-CRC-1 cells are evaluated in vitro using an MTT assay, DNA flow cytometry with propidium iodide, and Annexin V-FITC/propidium iodide staining, respectively. In vivo, the anti-angiogenic potential of VAN and CAB is evaluated in Tg(fli1a:EGFP)^y1 transgenic fluorescent zebrafish embryos by analyzing the effects on the physiological development of the sub-intestinal vein plexus and the tumor-induced angiogenesis after TT and MZ-CRC-1 xenotransplantation. VAN and CAB exert comparable effects on TT and MZ-CRC-1 viability inhibition and cell cycle perturbation, and stimulated apoptosis with a prominent effect by VAN in MZ-CRC-1 and CAB in TT cells. Regarding zebrafish, both drugs inhibit angiogenesis in a dose-dependent manner, in particular CAB shows a more potent anti-angiogenic activity than VAN. To conclude, although VAN and CAB show comparable antiproliferative effects in MTC, the anti-angiogenic activity of CAB appears to be more relevant.     

Centrifugal partition chromatography applied to the isolation of oak wood aroma precursors

Flavours extracted from oak wood during barrel ageing contribute to the organoleptic character of wines and spirits. The aim of this work was to identify the glycosidic precursors of the key volatile compounds responsible for oak wood aroma. Oak extract is a very complex matrix and, furthermore, precursors are present in very small quantities. Preparative centrifugal partition chromatography (CPC) is a promising solution for purifying the oak extract. The solvent system was selected on the basis of the partition coefficient of glycosidase enzyme activity (K_ca). Thanks to the efficacy of CPC separation, three glucoside gallates were subsequently isolated by HPLC chromatography. Vanillin-(6′-O-galloyl)-O-β-d-glucopyranoside, 3,4,5-trimethoxyphenyl-(6′-O-galloyl)-O-β-d-glucopyranoside, and (6R,9R)-3-oxo-α-ionol-9-O-(6′-O-galloyl)-β-glucopyranoside (macarangioside E) were isolated and identified. This was the first time that vanillin-(6′-O-galloyl)-O-β-d-glucopyranoside was identified and the first time that macarangioside E was isolated from oak wood. Heating macarangioside E resulted in the formation of megastigmatrienone, which has an aroma reminiscent of tobacco.     

BETaaS: A Platform for Development and Execution of Machine-to-Machine Applications in the Internet of Things

Wireless Personal Communications - The integration of everyday objects into the Internet represents the foundation of the forthcoming Internet of Things (IoT). Smart objects will be the building...     

Optimal design of Service Overlay Networks with economic and performance constraints

In the last years, Service Overlay Networks (SONs) have emerged as a promising means to address some of the issues (e.g. end‐to‐end QoS) affecting the current Internet and to favor the development and deployment of new value‐added Internet services. The deployment of an SON is a capital‐intensive investment, since bandwidth with certain QoS guarantees must be purchased from the individual network domains through bilateral Service Level Agreements. Thus, minimizing the economic cost of the logical end‐to‐end service delivery infrastructure is one of the key objectives for the SON provider. When a SON is aimed at end‐to‐end QoS provisioning, its topology must be designed so as to also satisfy the specific requirements of QoS‐sensitive applications. This paper deals with the problem of planning the SON topology in order to take into account both cost and QoS constraints. More specifically, the paper proposes a set of new algorithms for the design of an optimized SON topology, which minimizes the economic cost while simultaneously meeting bandwidth and delay constraints. A performance comparison among such algorithms is finally carried out. Copyright © 2009 John Wiley & Sons, Ltd.     

On the Spatial Distribution of Fingerprint Singularities

Fingerprint singularities play an important role in several fingerprint recognition and classification systems. Although some general relationships and constraints about the location of singularities in the different fingerprint classes are well known, to the best of our knowledge no statistical models have been developed until now. This paper studies the spatial distributions of singularity locations in nature and derives, from a representative dataset of labelled samples, the probability density functions of the four main fingerprint classes. The results obtained can be directly exploited to improve the accuracy of many techniques relying on the position of singularities, as confirmed by the results of two experiments on fingerprint classification and synthesis.     

Mitigation of Liquefaction-Induced Lateral Deformation in a Sloping Stratum: Three-dimensional Numerical Simulation

Finite-element (FE) simulations are increasingly providing a versatile environment for conducting lateral ground deformation studies. In this environment, mitigation strategies may be assessed in order to achieve economical and effective solutions. On the basis of a systematic parametric study, three-dimensional FE simulations are conducted to evaluate mitigation by the stone column (SC) and the pile-pinning approaches. Mildly sloping saturated cohesionless strata are investigated under the action of an applied earthquake excitation. For that purpose, the open-source computational platform OpenSees is employed, through a robust user interface that simplifies the effort-intensive pre- and postprocessing phases. The extent of deployed remediation and effect of the installed SC permeability are investigated. The influence of mesh resolution is also addressed. Generally, SC remediation was found to be effective in reducing the sand stratum lateral deformation. For a similar stratum with permeability in the silt range, SC remediation was highly ineffective. In contrast, pile pinning appeared to be equally effective for the sand and silt strata permeability scenarios. Overall, the conducted study highlights the potential of computations for providing insights toward the process of defining a reliable remediation solution.     

Reoptimization of the Shortest Common Superstring Problem

A reoptimization problem describes the following scenario: given an instance of an optimization problem together with an optimal solution for it, we want to find a good solution for a locally modified instance.     

Use of microfibrillated cellulose and dendritic copper for the elaboration of conductive films from water- and ethanol-based dispersions

In this study, microfibrillated cellulose (MFC) and dendritic copper were used as binder and conductive phase for the elaboration of self-standing conductive films and coatings. A filtration technique was used to prepare MFC/Cu films from particle dispersions in water and ethanol. In aqueous slurries copper oxidized and an additional corona treatment or the use of zinc particles as sacrificial anode were necessary to obtain films with conductivities ranging from 70 to 2500 S m^?1, respectively. In ethanol-based MFC/Cu slurries, copper was subjected to limited oxidation. However, the low packing density of conductive particles (below the percolation threshold) led to resistive films which, after densification by calendering, displayed extremely high conductivities, up to 70000 S m^?1. Aqueous MFC/Cu slurries were successfully used for the deposition of conductive coatings on copy paper by Mayer rod coating and screen printing, which were subsequently treated by corona discharge and calendering. The obtained coatings displayed intermediate conductivity (i.e. 95 and 570 S m^?1 for rod coating and screen printing, respectively), which can be further increased using zinc particles or ethanol-based formulations.