Comparison of the Translational Potential of Human Mesenchymal Progenitor Cells from Different Bone Entities for Autologous 3D Bioprinted Bone Grafts

Reconstruction of segmental bone defects by autologous bone grafting is still the standard of care but presents challenges including anatomical availability and potential donor site morbidity. The process of 3D bioprinting, the application of 3D printing for direct fabrication of living tissue, opens new possibilities for highly personalized tissue implants, making it an appealing alternative to autologous bone grafts. One of the most crucial hurdles for the clinical application of 3D bioprinting is the choice of a suitable cell source, which should be minimally invasive, with high osteogenic potential, with fast, easy expansion. In this study, mesenchymal progenitor cells were isolated from clinically relevant human bone biopsy sites (explant cultures from alveolar bone, iliac crest and fibula; bone marrow aspirates; and periosteal bone shaving from the mastoid) and 3D bioprinted using projection-based stereolithography. Printed constructs were cultivated for 28 days and analyzed regarding their osteogenic potential by assessing viability, mineralization, and gene expression. While viability levels of all cell sources were comparable over the course of the cultivation, cells obtained by periosteal bone shaving showed higher mineralization of the print matrix, with gene expression data suggesting advanced osteogenic differentiation. These results indicate that periosteum-derived cells represent a highly promising cell source for translational bioprinting of bone tissue given their superior osteogenic potential as well as their minimally invasive obtainability.     

Full-Field Strain Rate Measurement by White-Light Speckle Image Correlation

Abstract:  This paper explains a numerical procedure to process sequences of digital images and to return a full-field evaluation of the strain rate. The processing procedure is based on a nonlinear least squares fitting performed globally, on the whole image, and simultaneously on several images. The use of a highly optimised code allows the analysis of long sequences in a few minutes. The results of calculations are presented as movies built by blending the colour maps of the measured strain field with the specimen pictures used in the correlation procedure. Our application is focused on studying the plastic behaviour of metals and, in particular, on highlighting any transient phenomena that might occur during yielding and strain-hardening phases on thin sheets used in the manufacture of sheet metals. A typical example for such phenomena is the Portevin-Le Châtelier effect, a repetitive yielding of alloys during plastic deformation.     

Analytical and Field Investigation of Roma Suspension Bridge

The Roma–Ciudad Miguel Aleman International Suspension Bridge is an historic unstiffened suspension bridge with a 192 m (630 ft) main suspended span, originally constructed in 1928. In 1997 the bridge was inspected and a full-scale nondestructive load test was conducted. The resulting experimental data are evaluated and compared to the results of analyses by finite-element method modeling. The history of the bridge is reviewed, with an emphasis on modifications and retrofits to the structure. The unique behavior and attributes of unstiffened suspension bridges are discussed in the specific context of this particular bridge.     

Reliable changes in psychotherapy behavior among first-year psychiatric residents.

Recorded differences between 19 1st-yr psychiatric residents (novices) and 19 experienced psychotherapists during interviews with a standard film patient. In an initial interview, novices asked more questions/total number of statements than did experienced therapists and their statements contained relatively fewer words. During 2 subsequent interviews, these differences were not present and several lines of evidence suggest that their disappearance was not due to increased patient contact. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Censure sensitivity in schizophrenia.

Hypotheses derived from the Rodnick-Garmezy theory of schizophrenic censure sensitivity were tested in a 3 Groups (Normals and Poor and Good Premorbid Schizophrenics) × 2 Sex (Man and Woman Parental Surrogate) factorial design. Predictions were tested both at predispositional (recollections of parental child-rearing attitudes and practices) and behavioral levels (susceptibility to social influence). The results at both levels failed to support the predictions of general schizophrenic hypersensitivity and differential schizophrenic sensitivity to sex according to the adequacy of premorbid adjustment. The general clinical theory of hyposensitivity was considered as an alternative but was also found to be inadequate. Reported parental dominance was unrelated to susceptibility to influence. Acquiescence response tendency was found to be highly related to reports of parental attitudes and to extent of social influence. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Development and validation of the Neuropsychology Behavior and Affect Profile.

This series of 4 studies describes the psychometric properties of the Neuropsychology Behavior and Affect Profile, which consists of 5 peer-rated scales (106 items) designed to measure personality change in brain-impaired individuals. Study 1 pertains to item derivation. Study 2 used relatives of 61 Ss identified as demented to determine the test's internal consistency. Results showed moderate levels of internal consistency across the 5 scales, with slightly higher coefficients (.68–.82) obtained for present (vs. premorbid) emotional status. High test–retest reliability was demonstrated in Study 3 (intraclass correlation coefficients ranged from .92 to .99). Study 4 established discriminant validity; the instrument differentiated 61 demented Ss from 88 normal elderly controls on the basis of present behavioral affective style. (PsycINFO Database Record (c) 2010 APA, all rights reserved)