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991.
Retinoids [retinol (vitamin A) and its metabolites] function in the visual cycle, embryonic development, cellular differentiation, and tissue homeostasis. Notwithstanding pivotal roles of retinoids in mammals, the limited number of commercially available retinoid standards is a major roadblock to identifying and studying retinoids in biological samples. Therefore, a need exists for improved methods to identify retinoid metabolites. We analyzed polar and nonpolar retinoids, including retinoic acid, retinol, retinyl acetate, and other retinyl esters, using postsource decay laser desorption/ionization mass spectrometry (PSD-LDI MS). PSD analysis was employed to examine the PSD fragmentation patterns of retinoids, as these patterns can be used for the characterization of retinoids from biological samples without the need for matching retention time with a commercially available or synthetic retinoid. Mechanisms for the formation of these PSD fragment ions are proposed. The feasibility of employing PSD after HPLC separation was demonstrated by characterizing the endogenous retinoids in canine kidney epithelial cell extracts and in mouse lung. We show that the PSD-LDI MS approach described here can facilitate the identification and characterization of retinoids from mammalian cells and tissues. 相似文献
992.
Individual M13 viruses were spatially confined within wells fabricated from nanomolding of a PEG-based random copolymer. The viruses were selectively adhered to the region pretreated with an antibody against the virus, resulting in individual virus arrays. The polymer surface was found to be highly resistant to the attachment of the virus (approximately 0.02 microm-2), approximately 2 orders of magnitude lower than that on a bare silicon surface. The physical height of the template provided an additional barrier to the attachment of the virus due to entropic penalty in bending of a semi-flexible M13 virus. The effects of pattern size and barrier height were investigated, revealing that a certain critical height is needed to ensure successful confinement within the template for a given pattern size. 相似文献
993.
994.
G. H. Zhang R. Wei M. Enomoto D. W. Suh 《Metallurgical and Materials Transactions A》2012,43(3):833-842
The growth kinetics of proeutectoid ferrite in the early stages of transformation were studied in Fe-0.1C-1.5Mn-1Si (mass
pct) quaternary and Fe-0.1C-1.5Mn-1Si-0.2Al quinary alloys. The observed kinetic transition temperatures from partitioned
slow growth to unpartitioned fast growth of ferrite in both alloys are in good agreement with predictions using a local equilibrium
model for multicomponent systems. The measured parabolic growth rate constants were smaller than those calculated assuming
paraequilibrium in the unpartitioned growth region, but the difference between the measured and the calculated growth rate
constants gradually diminished with a decreasing reaction temperature. The dissipation of driving force, derived from the
diffusion of the substitutional solute within the transformation interface, possibly constitutes a major part of the discrepancy
between the measured and the calculated growth kinetics. 相似文献
995.
Yangyang Wang Yong Kweon Suh Sangmo Kang 《Journal of Mechanical Science and Technology》2009,23(3):874-884
In numerical studies on microscale electroosmotic flows, the electric double layer (EDL) effect is usually predicted by solving
the traditional Navier-Stokes equation subjected to the slip velocity induced by the electric-charged wall as a boundary condition.
Recently, Suh and Kang (Physical Review E 77, 2008) presented the asymptotic solutions of the ion transport equations near a polarized electrode under the action of an
AC field, and then proposed a new theoretical model of the slip velocity on the electrode considering the ion adsorption effect.
In the present paper, we apply the model to a two-dimensional AC-electroosmotic flow in a microchannel to calculate the slip
velocity on a pair of coplanar asymmetric electrodes embedded on the bottom wall, and then experimentally measure the slip
velocity using the micro-PIV technique to validate the theoretical model. Comparison shows an excellent overall match between
the theoretical and experimental results, except for on the narrow electrode at low frequencies. Next, we numerically perform
parametric studies regarding the AC frequency, effective Stern-layer thickness and ion adsorption effect to further understand
the characteristics of the AC electroosmotic flow. Results show that, as the frequency increases, the slip velocity also increases.
In addition, the velocity decreases with increasing either Stern-layer thickness or ion adsorption effect.
This paper was recommended for publication in revised form by Associate Editor Dongshin Shin
Sangmo Kang received a B.S. and M.S. degrees from Seoul National University in 1985 and 1987, respectively, and then had worked for five
years in Daewoo Heavy Industries as a field engineer. He also achieved a Ph.D. degree in the field of Mechanical Engineering
from the University of Michigan in 1996. Dr. Kang is currently a Professor at the Division of Mechanical Engineering at Dong-A
University in Busan, Korea. Dr. Kang’s research interests are in the area of micro- and nanofluidics and turbulent flow combined
with the computational fluid dynamics. 相似文献
996.
Nansai K Kagawa S Kondo Y Suh S Nakajima K Inaba R Oshita Y Morimoto T Kawashima K Terakawa T Tohno S 《Environmental science & technology》2012,46(1):155-163
In recent years, greenhouse gas emission controls that incorporate the supply chains of products and services, thereby emphasizing the role of consumers rather than producers, have been drawing increasing attention. A country's consumption-based emissions, including those due to global supply chains, reflect the total emissions on which the national economy relies. To design effective emissions control strategies there is therefore an urgent need for countries to elucidate the structural relationship between their domestic economy and emissions occurring through global supply chains. Here we consider the structural characteristics of consumption-based emissions in Japan, which in 2005 totaled 1675 Mt CO(2)eq. Outside the country the Japanese economy generated global emissions of 541 Mt CO(2)eq, 35.7% of which were UNFCCC Annex I emissions and 64.3% were non-Annex I and other emissions. This figure of 64.3% reveals that Japan is actually relying to a considerable degree on emissions that are subject to no international obligations. We identify key economic contributors to consumption-based emissions at the commodity level and specify items of household expenditure that are effective options for both financial savings and emissions reduction. We then discuss the importance of emissions control for evolving toward a "carbon-debt-free country". 相似文献
997.
Hajin Nam Harry Jung S. Karuppasamy Young Shik Park Yong Seok Cho Jae Yeon Lee Su-Il Seong Jun Gyo Suh 《Food science and biotechnology》2012,21(6):1669-1676
This study investigated the effects of soy bean extract fermented by Bacillus subtilis MORI (BTD-1) on blood glucose, hemoglobin A1c (HbA1c), plasma insulin, and pancreatic β islets in db/db mice. The BTD-1 (500 mg/kg) group showed significantly lower fasting blood glucose level (p<0.01) and postprandial 2 h blood glucose level (p<0.01) compared with the db control group. The BTD-1 (500 mg/kg) group showed significantly lower HbA1c level compared with the db control group (p<0.01). After 8 weeks of BTD-1 administration, the pancreatic islet architecture was preserved and the immunofluorescent intensities of insulin in BTD-1 (500mg/kg) group apparently increased compared to in the db control group. Plasma insulin levels were found to be significantly higher in the BTD-1 (500 mg/kg) group than in the db control group (p<0.05). In summary, our results suggest that BTD-1 has an anti-diabetes effect in a non-insulin dependent diabetes mellitus mouse model. 相似文献
998.
SR Vivekchand CJ Engel SM Lubin MG Blaber W Zhou JY Suh GC Schatz TW Odom 《Nano letters》2012,12(8):4324-4328
This paper reports the manipulation of surface plasmon polaritons (SPPs) in a liquid plasmonic metal by changing its physical phase. Dynamic properties were controlled by solid-to-liquid phase transitions in 1D Ga gratings that were fabricated using a simple molding process. Solid and liquid phases were found to exhibit different plasmonic properties, where light coupled to SPPs more efficiently in the liquid phase. We exploited the supercooling characteristics of Ga to access plasmonic properties associated with the liquid phase over a wider temperature range (up to 30 °C below the melting point of bulk Ga). Ab initio density functional theory-molecular dynamic calculations showed that the broadening of the solid-state electronic band structure was responsible for the superior plasmonic properties of the liquid metal. 相似文献
999.
Park JH Noh JH Han BS Shin SS Park IJ Kim DH Hong KS 《Journal of nanoscience and nanotechnology》2012,12(6):5091-5095
Niobium doped hierarchically organized TiO2 nanostructures composed of 20 nm size anatase nanocrystals were synthesized using pulsed laser deposition (PLD). The Nb doping concentration could be facilely controlled by adjusting the concentration of Nb in target materials. We could investigate the influence of Nb doping in the TiO2 photoelectrode on the cell performance of dye-sensitized solar cells (DSSCs) by the exclusion of morphological effects using the prepared Nb-doped TiO2 anostructures. We found no significant change in short circuit current density (Jsc) as a function of Nb doping concentration. However, open circuit voltage (Voc) and fill factor (FF) monotonously decrease with increasing Nb concentration. Dark current characteristics of the DSSCs reveal that the decrease in Voc and FF is attributed to the decrease in shunt resistance due to the increase in conductivity TiO2 by Nb doping. However, electrochemical impedance spectra (EIS) analysis at open circuit condition under illumination showed that the resistance at the TiO2/dye/electrolyte interface increases with Nb concentration, revealing that Nb doping suppress the charge recombination at the interface. In addition, electron life time obtained using characteristic frequency in Bode plot increases from 14 msec to 56 msec with increasing Nb concentration from 0 to 1.2 at%. This implies that the improved light harvesting can be achieved by increasing diffusion length through Nb-doping in the conventional TiO2 photoelectrode. 相似文献
1000.
To form the Cu circuit with electroless plating method on polyethylene terephthalate (PET) film, the Pd catalyst layer was formed by printing and liquid phase reduction of the Pd(II) ink on surface modified polyethylene terephthalate (PET) film. The Pd(II) ink could be obtained by hydrolysis of PdCl2 and NH4Cl solution and added with a stabilizing agent. To investigate the optimal printing characteristics by surface modification, the PET film was immersed into the KOH solution. The contact angle on surface of PET film greatly depended on the temperature and the concentration of the KOH solution. The printing of Pd(II) ink and Cu electroless plating characteristics were most excellent when the surface modification was carried out for 60 min at 60 degrees C in the 5-10 M KOH solution. 相似文献