Citizen Participation in Planning: The Relationship Between Objectives and Techniques

Problem: As Richard Florida's writings about the creative class garnered attention across the globe, planners and local government officials responded by enacting policies to attract and retain creative workers, often favoring spending for amenity and lifestyle attractions over more established economic development approaches. It is not clear, however, if the presence of these workers drives regional growth and development as effectively as more traditionally accepted place-based and institutional factors.

Purpose: In this article we explore the relationships between the presence of the creative class and regional economic performance, contrasting measures of regional creative capacity with traditional competitiveness factors.

Methods: We examine how Florida's creative class measures correlate with each other and with common indicators of economic performance for U.S. metropolitan areas. We also estimate multivariate regression models to compare the influence of Florida's measures to those of more traditional indicators of economic competitiveness on metropolitan job growth, income growth, and job instability.

Results and conclusions: We find that differences in Florida's measures of creativity are not generally associated with differences in metropolitan economic performance. Indicators of human capital and industry composition perform as well or better than talent, tolerance, and technology in explaining metropolitan job and income growth and job instability.

Takeaway for practice: Since we find measures derived from Florida's creative class hypotheses to be no more associated with positive economic outcomes than traditional competitiveness measures, we do not advocate replacing traditional economic development strategies with those based primarily on attracting the creative class. Programs supporting education, business creation, and industrial diversity are more likely to be effective tools for promoting economic well-being.

Research support: We received support from the Center for the Study of the American South and the Office of Economic and Business Development's Program on Southern Politics, Media, and Public Life, both of the University of North Carolina at Chapel Hill.     

Simulating Cities: Process,Product, and Prognosis

Problem, research strategy, and findings: Little research examines the effect of diversity on regional economic resilience to natural disasters. We examine whether economic diversity benefits regional economies in typical circumstances and in recovery after a natural disaster, using the case of the 1993 U.S. Midwest flood. By matching counties in the nine states affected by the flood to control counties, we isolate the influence of diversity on employment and income in normal circumstances and after a substantial shock. We found economic diversity to have mixed associations with employment and income in typical circumstances. On average, economically diverse counties tended to experience faster employment gains but slower growth in per capita income than less diverse areas. The effect of economic diversity upon resilience following a natural disaster was unambiguous. Economic diversity aided counties in weathering the downturn following the flood and sped their return to long-term patterns of employment and income growth.

Takeaway for practice: In promoting policies to enhance economic diversity, planners in communities located in areas prone to natural disasters should consider both the goal of disaster resilience and the potential tradeoffs between different aspects of economic performance.

Research support: None.     

Molecular species of phosphatidylethanolamine from continuous cultures of Saccharomyces pastorianus syn. carlsbergensis strains

Saccharomyces pastorianus syn. carlsbergensis strain 34/70 is well known to be the most used strain for lager beer production. The difference between this strain and very closely related strain 34/78 is the latter's greater flocculating character. This single physiological trait can cause technical difficulties in beer production. The aim of this study was to determine whether lipid analysis by a combination of thin layer chromatography (TLC) with electrospray ionization mass spectrometry (ESI-MS) could be used as a strain-typing technique in order to distinguish S. pastorianus syn. carlsbergensis strain 34/70 from strain 34/78. Both strains (34/70 and 34/78) were harvested after continuous culture under standard conditions. Polar lipids were then extracted from lyophilized cultures and analysed by TLC in order to separate phospholipid families. Phosphatidylethanolamine (PE) was extracted and investigated using ESI-MS, to gain further information on individual molecular species. Using TLC analysis, lipids were separated corresponding to standards for PE, phosphatidylcholine (PC), phosphatidylglycerol (PG), cardiolipin (CL), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA) and sphingomyelin (SM). ESI-MS of the PE band, separated by TLC, showed that electrospray mass spectra were highly reproducible for repeat cultures. Novel findings were that both brewing strains displayed major phospholipid peaks with m/z 714, PE (34 : 2) m/z 742, PE (36 : 2) and m/z 758, PE (37 : 1). However, strain 34/78 had additional peaks of m/z 700, PE (33 : 2) and m/z 728, PE (35 : 2). Strain 34/70 had an extra peak with m/z 686 PE (32 : 2). We conclude that combined TLC/ESI-MS can distinguish between S. pastorianus syn. carlsbergensis 34/70 and 34/78 and may be a useful typing technique for differentiation of closely related yeast strains. This novel approach may aid quality assurance and could be suitable for yeast collections and larger industrial companies.     

The MIDAS project at ASU: John Cowley's vision and practical results

An overview of the conception and development of the MIDAS system at Arizona State University is given: a Microscope for Imaging, Diffraction and Analysis of Surfaces. John Cowley's vision in the early 1980s was ambitious and far-reaching, and it was because of him the authors came to ASU. We were centrally involved in the design and implementation of MIDAS from the mid 1980s onwards; the novel design features are briefly reviewed. Practical results obtained using this instrument are listed, and the scope for future development and applications are indicated. While it is clear that many new results have been demonstrated, even more possibilities still remain to be explored. Some comments are made about the feasibility of such developments in the light of competing instrumentation.     

Polar Lipids of Brewer's Yeasts

Both traditional and DNA‐based methods sometimes fail to differentiate between closely related strains of commercial interest in the brewing industry. The aim of this study was to compare species and sub species of Saccharomyces cerevisiae on the basis of their polar lipid chemistry using chromatographic methods. Six isolates were studied after propagation under batch conditions. Polar lipids were then extracted from lyophilised cultures and analysed by TLC in order to separate phospholipid families. TLC showed that the major phospholipid classes present were PC > PE > PG. Two unidentified phospholipids were found, one only in strain 34/70. The major peaks detected by GLC were identified as methyl esters of palmitic acid and palmitoleic acid. The fatty acid composition of PC varied between strains and novel data on lecithin acyl constituents were observed. The polar lipid method succeeded in differentiating strain 34/70 — one of the most commonly used brewer's lager yeast — from strain 34/78 and other species tested. The presence of unusual polar lipids in Saccharomyces sensu stricto yeasts may be useful in distinguishing between other closely related strains.     

Phospholipid molecular species distribution of Porphyromonas asaccharolytica ATCC 25260T: effects of temperature, culture age and pH

The aim of this study was to determine the extent to which phospholipid molecular species profiles are affected by different environmental factors in Porphyromonas asaccharolytica ATCC 25260T. Phospholipids were analysed by Fast Atom Bombardment Mass Spectrometry (FAB-MS) in negative-ion mode. Under standard growth conditions (37 degrees C, pH 7.0, 48 h), the most intense high mass anions were m/z 653 and 662. The latter is consistent with the expected presence of PE (30:0). The only changes in profiles were quantitative. These were compared using the Pearson Coefficient of Linear Correlation. The r-values for initial pH comparisons ranged from 0.82 (pH 7.0 vs pH 6.0) to 1.00 (pH 5.0 vs pH 8.0), for incubation period, from 0.86 (48 vs 72 h) to 0.97 (96 vs 168 h), and for temperature, from 0.57 (40 vs 37 degrees C) to 0.96 (37 vs 36 degrees C). Differences were also seen when plates were incubated in anaerobe jars as opposed to an anaerobic work station (r = 0.75). It is concluded that it is essential to standardize growth parameters, and to use an anaerobe jar or an anaerobe work station, but not both.     

Refinery: Visual Exploration of Large,Heterogeneous Networks through Associative Browsing

Browsing is a fundamental aspect of exploratory information‐seeking. Associative browsing represents a common and intuitive set of exploratory strategies in which users step iteratively from familiar to novel bits of information. In this paper, we examine associative browsing as a strategy for bottom‐up exploration of large, heterogeneous networks. We present Refinery, an interactive visualization system informed by guidelines for associative browsing drawn from literature on exploratory information‐seeking. These guidelines motivate Refinery's query model, which allows users to simply and expressively construct queries using heterogeneous sets of nodes. This system computes degree‐of‐interest scores for associated content using a fast, random‐walk algorithm. Refinery visualizes query nodes within a subgraph of results, providing explanatory context, facilitating serendipitous discovery, and stimulating continued exploration. A study of 12 academic researchers using Refinery to browse publication data demonstrates how the system enables discovery of valuable new content, even within existing areas of expertise.