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21.
Properties and applications of β‐glycosidase from Bacteroides thetaiotaomicron that specifically hydrolyses isoflavone glycosides
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Da‐Hye Byun Hye‐Jeong Choi Hye‐Won Lee Hye‐Yeon Jeon Woo‐Jae Choung Jae‐Hoon Shim 《International Journal of Food Science & Technology》2015,50(6):1405-1412
To modify the glycan part of glycosides, the gene encoding β‐glycosidase was cloned from Bacteroides thetaiotaomicron VPI‐5482. The cloned gene, bt_1780, was expressed in Escherichia coli MC1061 and the expressed enzyme was purified using Ni‐NTA affinity chromatography. The purified enzyme, BTBG, showed optimal activity at 50 °C and pH 5.5. Interestingly, this enzyme did not have any hydrolysing activity on ordinary β‐linkage–containing substrates such as xylobiose, lactose and cello‐oligosaccharide, but specifically hydrolysed isoflavone glycosides such as daidzin, genistin and glycitin. Compared to a commercial beta glucosidase, BTBG selectively hydrolysed isoflavone glycosides in soybean extract mixture solution. These results suggest that BTBG may be a specialized enzyme for the hydrolysis of glycosides and that the substrate specificity of BTBG is applicable for the bioconversion of isoflavone glycosides in the food industry. 相似文献
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Koo Min-Sung Choi Ho-Lim 《International Journal of Control, Automation and Systems》2020,18(9):2186-2194
International Journal of Control, Automation and Systems - In this paper, a regulation problem for a class of lower triangular nonlinear systems under unknown measurement sensitivity by output... 相似文献
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Jinho Choi Sanghun Jung Deok Gun Park Jaegul Choo Niklas Elmqvist 《Computer Graphics Forum》2019,38(3):249-260
The majority of visualizations on the web are still stored as raster images, making them inaccessible to visually impaired users. We propose a deep‐neural‐network‐based approach that automatically recognizes key elements in a visualization, including a visualization type, graphical elements, labels, legends, and most importantly, the original data conveyed in the visualization. We leverage such extracted information to provide visually impaired people with the reading of the extracted information. Based on interviews with visually impaired users, we built a Google Chrome extension designed to work with screen reader software to automatically decode charts on a webpage using our pipeline. We compared the performance of the back‐end algorithm with existing methods and evaluated the utility using qualitative feedback from visually impaired users. 相似文献
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Ha-Nul Lee Joo-Hee Choi Ji-Yeon Park Jae-Hun Ahn Da Eun Jang Jae Gun Shim Jong-Hwan Park Young-Min Kim 《Food science and biotechnology》2021,30(4):583
Vegetable soup (VS), a plant-based functional food, has been used as a traditional folk medicine and is attracting attention for its ability to enhance the immune response. β-Glucan, a well-established and effective immunomodulator, has synergistic effects when used in combination with some bioactive compounds. In the present study, we aimed to evaluate the synergistic immunomodulatory effects of the combination of VS and β-glucan on macrophage-mediated immune responses. β-Glucan was demonstrated to synergistically enhance the VS-stimulated immune response, including the production of interleukin-6, tumor necrosis factor-α, and nitric oxide, mainly through the mitogen-activated protein kinase pathway in macrophages. In addition, this combination has the potential for further development in functional foods with immune-enhancing activity.Supplementary InformationThe online version contains supplementary material available at 10.1007/s10068-021-00888-x. 相似文献
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Co-browsing is an activity in which a group of users navigate their way through of a set of Web pages together for a shared
purpose. Effective co-browsing among users with different device capabilities requires a shared understanding of those Web
pages. This paper demonstrates the concept of shared viewpoints (SVPs), and personal viewpoints (PVPs), for co-browsing, before
detailing a framework for implementing these concepts. Finally, the effectiveness of the framework is presented through a
perceptual experiment. 相似文献
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S.J. Choi K. Djordjev Zhen Peng Qi Yang Sang Jun Choi P.D. Dapkus 《Photonics Technology Letters, IEEE》2004,16(10):2266-2268
All-buried InP-InGaAsP ring resonators laterally coupled to bus waveguides are demonstrated. The buried configurations offer a lower built-in refractive index step along the resonator periphery, which affords enhanced optical coupling coefficients between the waveguides and reduced scattering losses caused by the resonator sidewall imperfections. Very low optical intensity attenuations of 0.4 cm/sup -1/ and coupling-limited quality factors of greater than 10/sup 5/ are observed from 200-/spl mu/m-radii ring resonators. The measured spectral linewidth is as narrow as 0.0145 nm. 相似文献