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101.
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Sarka Jelinkova Yvonne Sleiman Petr Fojtík Franck Aimond Amanda Finan Gerald Hugon Valerie Scheuermann Deborah Beckerov Olivier Cazorla Marie Vincenti Pascal Amedro Sylvain Richard Josef Jaros Petr Dvorak Alain Lacampagne Gilles Carnac Vladimir Rotrekl Albano C. Meli 《International journal of molecular sciences》2021,22(9)
Duchenne muscular dystrophy (DMD) is a devastating condition shortening the lifespan of young men. DMD patients suffer from age-related dilated cardiomyopathy (DCM) that leads to heart failure. Several molecular mechanisms leading to cardiomyocyte death in DMD have been described. However, the pathological progression of DMD-associated DCM remains unclear. In skeletal muscle, a dramatic decrease in stem cells, so-called satellite cells, has been shown in DMD patients. Whether similar dysfunction occurs with cardiac muscle cardiovascular progenitor cells (CVPCs) in DMD remains to be explored. We hypothesized that the number of CVPCs decreases in the dystrophin-deficient heart with age and disease state, contributing to DCM progression. We used the dystrophin-deficient mouse model (mdx) to investigate age-dependent CVPC properties. Using quantitative PCR, flow cytometry, speckle tracking echocardiography, and immunofluorescence, we revealed that young mdx mice exhibit elevated CVPCs. We observed a rapid age-related CVPC depletion, coinciding with the progressive onset of cardiac dysfunction. Moreover, mdx CVPCs displayed increased DNA damage, suggesting impaired cardiac muscle homeostasis. Overall, our results identify the early recruitment of CVPCs in dystrophic hearts and their fast depletion with ageing. This latter depletion may participate in the fibrosis development and the acceleration onset of the cardiomyopathy. 相似文献
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W. E. Woldt B. I. Dvorak M. F. Dahab 《Soft Computing - A Fusion of Foundations, Methodologies and Applications》2003,7(6):419-433
This research describes a framework and case study application that merges fuzzy set methods, pollution prevention, and sustainable
production concepts. There is a direct linkage between industrial pollution prevention, sustainability, and the solution of
large-scale environmental problems. This linkage stems from the inherent desire for economic production, while at the same
time protecting the environment from further degradation. The methodology combines systems analysis under imprecise conditions
with a life cycle assessment method that is able to accept imprecise data. Analysis of systems under imprecise conditions
is accomplished through analysis of process flow diagrams using fuzzy set techniques. Introduction of imprecision into life
cycle assessment is accomplished by integration of fuzzy set approaches into a decision support system utilizing multiple
criteria decision making. The framework is described and a case study application of an industrial parts cleaning system using
an open top vapor degreaser is presented. Results of applying the method show that: (1) It is well suited for analysis of
complex systems in which input data is sparse and expensive to collect. (2) The proposed framework includes a decision support
system that is able to consider life cycle assessment concepts, and is able to reconcile differing opinions on available options
for modification of production systems, thereby leading to more sustainable solutions.
The authors would like to acknowledge the USEPA Region VII Pollution Prevention Division, and the Pollution Prevention Incentives
for States program, for their support of this research. In addition, the authors would like to recognize the support of the
University of Nebraska Water Resources Center and Center for Infrastructure Research. The authors would also like to thank
Dan Crist and Yuezhang Wang for their assistance with fuzzy systems modeling. 相似文献
106.
JohnC.Dvorak 《个人电脑》2003,9(4):147-147
名字之谜。我看英特尔又给它的产品想出了一个怪名字,这就是刚宣布的Centrino(中文名“迅驰”),英特尔最新的包括802.11b协议支持的芯片。我猜这是英特尔的一些构词专家们共同琢磨出来的。显然这个名字来自某些词语的组合。诸如中心的(centric),中央的(central),中微子(neutrino)甚至圣婴(bambino)等等。 相似文献
107.
A novel procedure is proposed for evaluation of stress intensity factors of planar Mode III shear cracks perpendicular to
a nearby interface between two isotropic elastic solids. Shear cracks traversing a flat layer bonded to two different elastic
solids are also analyzed. The method is based on superposition of singular near tip stress and displacement fields generated
by both the main crack and certain image cracks. Both the main and the image cracks are loaded by self-equilibrating shear
tractions of different magnitude, such that matching parts of the said fields are made to satisfy traction and displacement
continuity conditions at the interface. Selected comparisons with results obtained by different methods show good agreement.
Applications of the method to other crack problems are discussed. 相似文献
108.
This paper is concerned with the development of constitutive equations for fibrous composites which contain a family of longitudinal slit cracks. The general theory in which the crack length is of the same order as the fiber diameter gives rise to a three-phase model. When the fiber diameter is much smaller than the crack length, we obtain the associated two-phase model. Detailed numerical results are given for selected systems. 相似文献
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The application of the atomic force microscope to studies of medically important protozoan parasites 总被引:1,自引:0,他引:1
Dvorak JA Kobayashi S Abe K Fujiwara T Takeuchi T Nagao E 《Journal of electron microscopy》2000,49(3):429-435
Both living and fixed specimens of the medically-important parasitic protozoa, Trypanosoma cruzi, Toxoplasma gondii, Giardia lamblia, Entamoeba histolytica, and Acanthamoeba spp. were studied by atomic force microscopy (AFM). The preparation of fixed specimens was similar to methods used for either scanning or transmission electron microscopy. AFM scanning was performed using both contact and tapping modes. A classical fixation procedure utilizing glutaraldehyde followed by ethanol dehydration was not suitable for all parasite species. AFM images could not be obtained from fixed samples of T. cruzi, T. gondii or E. histolytica. However, excellent topographic images could be obtained from specimens of G. lamblia and Acanthamoeba under identical conditions. Critical point drying permitted AFM imaging of both trypomastigote and epimastigote stages of T. cruzi. Phase imaging of T. cruzi elucidated unique surface details at a level of resolution not visible using any other imaging modalities. AFM elasticity map imaging of T. cruzi-infected and T. gondii-infected cells demonstrated that both parasites were markedly firmer than the surrounding host cell cytoplasm. The parasitophorous vacuole surrounding replicating T. gondii tachyzoites was also visualized by elasticity map imaging. These data suggest that although much remains to be learned about preparing parasitic protozoa for AFM imaging, the technique has the potential of providing unique and important insights into these disease causing organisms. 相似文献