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排序方式: 共有596条查询结果,搜索用时 18 毫秒
111.
Hepatic and Adipose Tissue Depot-Specific Changes in Lipid Metabolism in Late-Onset Obese (LOB) Rats
Transgenic Late-onset OBesity (LOB) rats slowly develop a male-specific, autosomal dominant, obesity phenotype with a specific
increase in peri-renal white adipose tissue (WAT) depot and preserved insulin sensitivity (Bains et al. in Endocrinology 145:2666–2679,
2004). To better understand the remarkable phenotype of these rats, the lipid metabolism was investigated in male LOB and non-transgenic
(NT) littermates. Total plasma cholesterol (C) levels were normal but total plasma triacylglycerol (TAG) (2.8-fold) and hepatic
TAG content (25%) was elevated in LOB males. Plasma VLDL-C and VLDL-TAG levels were higher while plasma apoB levels were 60%
lower in LOB males. Increased hepatic TAG secretion explained the increased VLDL levels in LOB males. The hepatic gene expression
of FAS, SCD-1, mitochondrial (mt)GPAT, and DGAT2 was up-regulated in both old obese and young non-obese LOB rats. Lipoprotein
lipase (LPL) activity in heart and epididymal white adipose tissue (WAT) was unchanged, while LPL activity was increased in
peri-renal WAT (30%) and decreased in soleus muscle (40%). Moreover, FAS, SCD-1 and DGAT2 gene expression was increased in
peri-renal, but not in epididymal WAT. Basal lipolysis was reduced or unchanged and β-adrenergic stimulated lipolysis was
reduced in WAT from both old obese and young non-obese LOB rats. To summarize, the obese phenotype of LOB male rats is associated
with increased hepatic TAG production and secretion, a shift in LPL activity from skeletal muscle to WAT, reduced lipolytic
response in WAT depots and a specific increase in expression of genes responsible for fatty acid and TAG synthesis in the
peri-renal depot.
F. Frick and R. Hume contributed equally to this work. 相似文献
112.
113.
Bezanson G Delaquis P Bach S McKellar R Topp E Gill A Blais B Gilmour M 《Journal of food protection》2012,75(3):480-487
Little is known about the influence of abiotic factors such as climate and soil chemistry on the survival of Escherichia coli O157:H7 in field lettuce. We applied a nalidixic acid-resistant derivative of strain ATCC 700728 to field-grown romaine lettuce in two regions in Canada characterized by large variances in soil type and climate. Surviving populations in soil and on lettuce leaves were estimated on sorbitol MacConkey agar supplemented with nalidixic acid. Data were fitted with the Weibull decline function to permit comparison of decay rates in the two experimental sites. E. coli O157:H7 populations fell from 10? to <102 CFU/g on leaves, and <103 CFU/g in soil within 7 days after inoculation. Analysis revealed there was no significant difference between decay rates at the two experimental sites in either environment. The results of this study suggest that the inherent ecological fitness of E. coli O157:H7 ATCC 700728 determines the extent of survival in the production environment. 相似文献
114.
Furuno S Foss S Wild E Jones KC Semple KT Harms H Wick LY 《Environmental science & technology》2012,46(10):5463-5470
To cope with heterogeneous subsurface environments mycelial microorganisms have developed a unique ramified growth form. By extending hyphae, they can obtain nutrients from remote places and transport them even through air gaps and in small pore spaces, repectively. To date, studies have been focusing on the role that networks play in the distribution of nutrients. Here, we investigated the role of mycelia for the translocation of nonessential substances, using polycyclic aromatic hydrocarbons (PAHs) as model compounds. We show that the hyphae of the mycelial soil oomycete Pythium ultimum function as active translocation vectors for a wide range of PAHs. Visualization by two-photon excitation microscopy (TPEM) demonstrated the uptake and accumulation of phenanthrene (PHE) in lipid vesicles and its active transport by cytoplasmic streaming of the hyphae ('hyphal pipelines'). In mycelial networks, contaminants were translocated over larger distances than by diffusion. Given their transport capacity and ubiquity, hyphae may substantially distribute remote hydrophobic contaminants in soil, thereby improving their bioavailability to bacterial degradation. Hyphal contaminant dispersal may provide an untapped potential for future bioremediation approaches. 相似文献
115.
Le Huy; Oh In-Sue; Robbins Steven B.; Ilies Remus; Holland Ed; Westrick Paul 《Canadian Metallurgical Quarterly》2011,96(1):113
The relationships between personality traits and performance are often assumed to be linear. This assumption has been challenged conceptually and empirically, but results to date have been inconclusive. In the current study, we took a theory-driven approach in systematically addressing this issue. Results based on two different samples generally supported our expectations of the curvilinear relationships between personality traits, including Conscientiousness and Emotional Stability, and job performance dimensions, including task performance, organizational citizenship behavior, and counterproductive work behaviors. We also hypothesized and found that job complexity moderated the curvilinear personality–performance relationships such that the inflection points after which the relationships disappear were lower for low-complexity jobs than they were for high-complexity jobs. This finding suggests that high levels of the two personality traits examined are more beneficial for performance in high- than low-complexity jobs. We conclude by discussing the implications of these findings for the use of personality in personnel selection. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
116.
Pepich BV Prakash B Domino MM Dattilio TA Munch DJ Price EK 《Environmental science & technology》2005,39(13):4996-5004
Method 527 was developed to address the occurrence monitoring needs of the U.S. Environmental Protection Agency (EPA) under its second unregulated contaminant monitoring rule (UCMR 2). This method includes a wide range of semivolatile organic contaminants, including pesticides that were deferred during the first UCMR, flame retardants, and pyrethroid pesticides. This paper discusses the rationale for selection and inclusion of the various contaminants included in Method 527 and describes the challenges associated with developing analytical methods that will be used for the occurrence monitoring of such a diverse group of organic molecules. Method 527 employs solid-phase extraction with analysis by gas chromatography/ mass spectrometry (GC/MS). The final method preservation scheme requires the storage of samples in amber bottles buffered at pH 3.8 using citric acid to prevent degradation from acid-catalyzed hydrolysis and from UV light. Citric acid is also an effective antimicrobial reagent, preventing this mode of loss during storage. Ethylenediaminetetraacetic acid (EDTA) is added to remove transition metals such as copper, which was determined to degrade target analytes upon storage. Finally, free available chlorine (FAC), which is present in many finished waters and found to degrade a number of the targets, is removed using ascorbic acid. The final method meets all of the EPA UCMR survey requirements for sample storage, precision, accuracy, and sensitivity and will be proposed for use under the UCMR 2. 相似文献
117.
Developing high‐quality, error‐free message‐passing concurrent programs is not trivial. Although a number of different primitives with associated semantics are available to assist such development, they often increase the complexity of the testing process. In this paper, we extend our previous test model for message‐passing programs and present new structural testing criteria, taking into account additional features used in this paradigm, such as collective communication, non‐blocking sends, distinct semantics for non‐blocking receives, and persistent operations. Our new model also recognizes that sender primitives cannot always be matched with every receive primitive. This improvement allows us to remove statically a significant number of infeasible synchronization edges that would otherwise have to be analyzed later by the tester. In this paper, the test model is presented using the Message‐Passing Interface standard; however, our new model has been designed to be flexible, and it can be configured to support a range of different message‐passing environments or languages. We have carried out case studies showing the applicability of the new test model to represent message‐passing programs and also to reveal errors, mainly those errors related to inter‐process communication. In addition to increasing the number of features supported by the test model, we have also reduced the overall cost of testing significantly. Our case studies suggest that the number of synchronization edges can be reduced by up to 93%, mainly by eliminating infeasible edges between unmatchable communication primitives. The main contribution of the paper is to present a more flexible test model that provides improved coverage for message‐passing programs and at the same time reduces the cost of testing significantly. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
118.
Pim van Ooij Annetje Guédon Henk A. Marquering Joppe J. Schneiders Charles B. Majoie Ed van Bavel Aart J. Nederveen 《Magma (New York, N.Y.)》2013,26(3):261-270
Objective
The objective of this study was to investigate the performance of k-t BLAST (Broad-use Linear Acquisition Speed-up Technique) accelerated time-resolved 3D PC-MRI compared to SENSE (SENSitivity Encoding) acceleration in an in vitro and in vivo intracranial aneurysm.Materials and methods
Non-accelerated, SENSE and k-t BLAST accelerated time-resolved 3D PC-MRI measurements were performed in vivo and in vitro. We analysed the consequences of various temporal resolutions in vitro.Results
Both in vitro and in vivo measurements showed that the main effect of k-t BLAST was underestimation of velocity during systole. In the phantom, temporal blurring decreased with increasing temporal resolution. Quantification of the differences between the non-accelerated and accelerated measurements confirmed that in systole SENSE performed better than k-t BLAST in terms of mean velocity magnitude. In both in vitro and in vivo measurements, k-t BLAST had higher SNR compared to SENSE. Qualitative comparison between measurements showed good similarity.Conclusion
Comparison with SENSE revealed temporal blurring effects in k-t BLAST accelerated measurements. 相似文献119.
Goldberg HI Neighbor WE Hirsch IB Cheadle AD Ramsey SD Gore E 《The Joint Commission journal on quality improvement》2002,28(4):155-166
BACKGROUND: The design of delivery systems that can truly conduct continuous quality improvement (CQI) as a routine part of clinical care provision remains a vexing problem. The effectiveness of the "computerized firm system" approach to chronic disease CQI was examined, with diabetes as the focus of a 5-year case study. METHODS: A large family medical center had been divided into two parallel group practices for reasons of efficiency. These frontline structures (also known as primary care "firms") were supported to serially adapt and evaluate selected CQI interventions by first introducing process changes on one firm but not the other and comparing the groups. Because all the required longitudinal data were contained in a computerized repository, it was possible to conduct these controlled "firm trials" in a matter of months at low cost. RESULTS: During a 3-year period, implementation of point-of-service reminders and a pharmacist out-reach program increased recommended glycohemoglobin (HbA1c) testing by 50% (p = 0.02) and reduced the number of diabetic patients inadequately controlled by 43% (p < 0.01). Following this outcome improvement, patients exhibited a 16% reduction in ambulatory visit rates (p = 0.04). The observed outcome improvement, however, was reversed during the subsequent 2 years, when staffing austerities forced by unrelated declines in clinic revenue caused the withdrawal of trial interventions. CONCLUSIONS: The processes and outcomes of diabetes care were improved, demonstrating that CQI and controlled trials are not mutually exclusive in moving toward the practice of evidence-based management. Health care systems can, by conducting serial firm trials, become learning organizations. CQI programs of all kinds will likely never flourish, however, until quality improvement and reimbursement mechanisms have become better aligned. 相似文献
120.
Paul Lieverse Pieter Van Der Wolf Kees Vissers Ed Deprettere 《The Journal of VLSI Signal Processing》2001,29(3):197-207
We present a methodology for the exploration of signal processing architectures at the system level. The methodology, named SPADE, provides a means to quickly build models of architectures at an abstract level, to easily map applications, modeled as Kahn Process Networks, onto these architecture models, and to analyze the performance of the resulting system by simulation. The methodology distinguishes between applications and architectures, and uses a trace-driven simulation technique for co-simulation of application models and architecture models. As a consequence, architecture models need not be functionally complete to be used for performance analysis while data dependent behavior is still handled correctly. We have used the methodology for the exploration of architectures and mappings of an MPEG-2 video decoder application. 相似文献