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51.
Xiang Jim; Sha Yuhua; Prasad Lata; Delbaere Louis T.J. 《Protein engineering, design & selection : PEDS》1996,9(6):539-543
Structural analysis derived from the crystallographic studyof the chimeric B72.3 antibody illustrated some major atomicinteractions between complementarity determining region (CDR)residues. For example, hydrogen bonds are formed between H35/H95,L50/H97, H53/H55 and H96/L96 respectively. These CDR residuesmay play important roles in the B72.3TAG72 (antibody-antigen)interaction either by direct interaction with the TAG72 antigenor by maintaining a CDR loop conformation through atomic interactionsbetween CDR residues. In order to confirm these assumptions,we altered these CDR residues by site-directed mutagenesis anddetermined binding affinities of these mutant chimeric antibodiesfor the TAG72 antigen in a solid-phase radioimmunoassay. Wefound that H55, H95, H97 and L96 are important CDR residuesfor the B72.3TAG72 interaction. Single amino acid substitutionsof aspartic acid and serine by alanine at H55 of CDR2 and atH95 of CDR3 respectively and of tyrosine by phenylalanine atH97 and L96 of CDR3, significantly reduced the binding affinityfor the TAG72 antigen by 20-, 8-, 16- and 45-fold respectively.Therefore, this study reveals some of the requirements for maintainingthe integrity of the B72.3 antibody combining sites. 相似文献
52.
介绍了硫酸生产中能量利用及我国硫酸工业能源利用状况。分析了硫酸工艺和设备选择中的节能降耗问题,做好投资费用与能耗的技术经济评估及塔设备、干吸塔除雾器、主鼓风机等主要耗能设备的设计与选型,合理地选择工艺、设备及其参数,提高能量利用率,实现投资最小化和节能最大化,构筑“节能型”硫酸工业。 相似文献
53.
Antioxidant activity of grape extracts in a lecithin liposome system 总被引:15,自引:0,他引:15
Ock-Sook Yi Anne S. Meyer Edwin N. Frankel 《Journal of the American Oil Chemists' Society》1997,74(10):1301-1307
Extracts of 14 different grapes were tested for their antioxidant activities in a copper-catalyzed lecithin liposome oxidation
assay and analyzed for their phenolic components by high-performance liquid chromatography (HPLC). The total phenolic contents
of the grape extracts varied from 176 to 1236 mg gallic acid equivalents (GAE)/L. Extracts of red wine grape varieties contained
higher concentrations of phenolics than other varieties. When compared at the same 20 μM GAE basis, the grape extracts inhibited
formation of conjugated diene hydroperoxides by 25.1 to 67.9%, and hexanal formation by 49.3 to 97.8%. Extracts of red table
grape varieties Red Globe and Emperor and white wine grape varieties Chardonnay and Sauvignon Blanc gave the highest antioxidant
activities. The relative percentage inhibition of conjugated dienes and hexanal correlated with total phenols (r=0.86 and 0.89). HPLC analyses showed that anthocyanins were the most abundant phenolic compounds in extracts of red grapes,
and flavonols were most abundant in extracts of white grapes. 相似文献
54.
55.
Sheldon M. Widerhorn Edwin R. Fuller Jr. 《Journal of the American Ceramic Society》1989,72(2):248-251
Data on the growth of cracks tested in aqueous solutions were interpreted in terms of surface force theory. For applied stress intensity factors greater than 0.25 MPa · m½, the position and the slope of the curves and their dependence on pH and ion concentration can be explained in terms of surface force theory, provided these forces are of a magnitude and range that are representative of those involved in the cohensive bonding of solids. Weaker forces, such as structural, double-layer, or dispersion forces, have little effect on crack growth in silica glass for K I > 0.25 MPa · m½. 相似文献
56.
A block copolymer nanotemplate for mechanically tunable polarized emission from a conjugated polymer
A polymer blend system consisting of polystyrene grafted onto poly (p-phenylene ethynylene) (PS-g-PPE) and poly (styrene-block-isoprene-block-styrene) triblock copolymer (SIS) yields highly polarized emission due to the unidirectional alignment of the PPE molecules. During the roll casting, the triblock copolymer microphase separates and creates unidirectionally aligned PS cylindrical microdomains in the rubbery PI matrix. PPE, a fluorescent conjugated polymer, was grafted with polystyrene (PS) side chains that enabled sequestration and alignment of these rigid backbone emitter molecules into the PS microdomains of the SIS triblock copolymer. Deforming the thermoplastic elastomer in a direction perpendicular to the orientation direction of the cylinders causes rotation of the PS cylinders and the PPE emitter molecules and affords tunable polarized emission due to re-orientation of the PPE containing PS cylinders as well as film thinning from Poisson effect. 相似文献
57.
In this paper we aim to characterize graphs in terms of a structural measure of complexity. Our idea is to decompose a graph into layered substructures of increasing size, and then to measure the information content of these substructures. To locate dominant substructures within a graph, we commence by identifying a centroid vertex which has the minimum shortest path length variance to the remaining vertices. For each graph a family of centroid expansion subgraphs is derived from the centroid vertex in order to capture dominant structural characteristics of the graph. Since the centroid vertex is identified through a global analysis of the shortest path length distribution, the expansion subgraphs provide a fine representation of a graph structure. We then show how to characterize graphs using depth-based complexity traces. Here we explore two different strategies. The first strategy is to measure how the entropies on the centroid expansion subgraphs vary with the increasing size of the subgraphs. The second strategy is to measure how the entropy differences vary with the increasing size of the subgraphs. We perform graph classification in the principal component space of the complexity trace vectors. Experiments on graph datasets abstracted from some bioinformatics and computer vision databases demonstrate the effectiveness and efficiency of the proposed graph complexity traces. Our methods are competitive to state of the art methods. 相似文献
58.
De-Long Zhang Ping-Rang Hua Edwin Yue-Bun Pun Guei-Gu Siu 《Journal of the American Ceramic Society》2007,90(9):2893-2899
Vapor transport equilibration (VTE) treatments were performed on a Y-cut bulk Er (1.6 mol%)-doped congruent LiNbO3 crystal and an X-cut pure congruent crystal, on one surface of which a 40 nm-thick film of erbium metal was coated before the VTE treatment. Scanning electron microscope, powder or single-crystal X-ray diffraction (XRD), polarized infrared absorption/emission of Er3+ as well as micro-Raman spectroscopy were used to study the two VTE crystals. The results are discussed in comparison with a corresponding as-grown bulk Er-doped crystal, calcined ErNbO4 powder, and a locally Er-doped congruent LiNbO3 crystal prepared by using the standard Er-diffusion technique. The experimental results show that the VTE treatment induces the formation of micrometer-sized ErNbO4 precipitates with the crystallographic morphology of a flat polyhedron not only on the surfaces of both crystals but also in the bulk of the homogeneously Er-doped one. The optical absorption and emission studies show that the formation of the precipitates results in substantial spectral changes in both the 0.98 and 1.5 μm regions. The micro-Raman studies allow to resolve four additional Raman peaks around 800 cm−1 in the E(TO) spectra of the two VTE crystals. These additional Raman peaks are associated with the characteristic vibrations with respect to the NbO4 3− group. Characteristic XRD, optical absorption, and emission and Raman peaks for identifying the ErNbO4 phase are proposed. Finally, the formation mechanism and light-scattering effect of the precipitates are discussed. 相似文献
59.
Effect of natural antioxidants in virgin olive oil on oxidative stability of refined,bleached, and deodorized olive oil 总被引:1,自引:0,他引:1
M. Teresa Satue Shu-Wen Huang Edwin N. Frankel 《Journal of the American Oil Chemists' Society》1995,72(10):1131-1137
The factors influencing the oxidative stability of different commercial olive oils were evaluated. Comparisons were made of
(i) the oxidative stability of commercial olive oils with that of a refined, bleached, and deodorized (RBD) olive oil, and
(ii) the antioxidant activity of a mixture of phenolic compounds extracted from virgin olive oil with that of pure compounds
andα-tocopherol added to RBD olive oil. The progress of oxidation at 60°C was followed by measuring both the formation (peroxide
value, PV) and the decomposition (hexanal and volatiles) of hydroperoxides. The trends in antioxidant activity were different
according to whether PV or hexanal were measured. Although the virgin olive oils contained higher levels of phenolic compounds
than did the refined and RBD oils, their oxidative stability was significantly decreased by their high initial PV. Phenolic
compounds extracted from virgin olive oils increased the oxidative stability of RBD olive oil. On the basis of PV, the phenol
extract had the best antioxidant activity at 50 ppm, as gallic acid equivalents, but on the basis of hexanal formation, better
antioxidant activity was observed at 100 and 200 ppm.α-Tocopherol behaved as a prooxidant at high concentrations (>250 ppm) on the basis of PV, but was more effective than the
other antioxidants in inhibiting hexanal formation in RBD olive oil.o-Diphenols (caffeic acid) and, to a lesser extent, substitutedo-diphenols (ferulic and vanillic acids), showed better antioxidant activity than monophenols (p- ando-coumaric), based on both PV and hexanal formation. This study emphasizes the need to measure at least two oxidation parameters
to better evaluate antioxidants and the oxidative stability of olive oils. The antioxidant effectiveness of phenolic compounds
in virgin olive oils can be significantly diminished in oils if their initial PV are too high. 相似文献
60.
针对现有聚集数据调度近似算法具有较高延时上界的问题,提出一种改进的聚集数据调度近似算法。建立一棵根在中心结点的广度优先搜索树,分层构造一个最大独立集(MIS),使MIS中相邻的2个结点相距两跳。将MIS中的结点连接起来,形成一棵根在中心结点的数据聚集调度树,使结点按数据聚集调度树进行分层数据调度。在数据聚集调度树的构造过程中,对于任意支配点,以最小的结点连接其相距两跳的支配点。对于2个相邻支配点的公共邻居支配点,通过在距中心点最近的支配点加入数据聚集树,使其在数据调度过程中将数据发送给距中心点最近的支配点,从而降低数据的聚集延时。实验结果表明,与SAS算法、Guo’s算法和IAS算法相比,该算法的数据聚集延时更低,其延时上界为14R+△?10。 相似文献