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31.
32.
An automatic quadrature algorithm especially designed for double integration of functions with some form of singular behaviour on the boundary of the integration region is described, and its FORTRAN code is presented. The algorithm is based on the use of the product trapezoidal rule, after a non-linear transformation of the integrand in both variables renders a new integrand function whose derivatives vanish on the (transformed) boundary. Numerical results demonstrate the ability of the algorithm to obtain high accuracies in dealing automatically with pathological singularities of non-specific types. 相似文献
33.
Data are given for shear and tensile testing of adhesive bonds under conditions of a constant rate of loading. A rate equation is then used to predict lifetime from the mechanical data. The correlations appear to be satisfactory providing that the failure is cohesive within the adhesive. 相似文献
34.
Cissé Mohamed N'guessan Elise Tia V. Etienne Gérard Loiseau Didier Montet 《Journal of Food Safety》2020,40(3):e12785
Lactoperoxidase system (LPOS) was incorporated into chitosan solutions (0.5, 1, and 1.5%) to protect mangoes against two strains of fungi. Coating solutions effectiveness in vitro (in Petri dish) and in vivo (on mango) were studied on fungal (Phomopsis sp. RP257 and Pestalotiopsis sp.) growth isolated from mango cv Amelie. In vitro, chitosan concentration at least 1% containing or not LPOS effectively inhibited Pestalotiopsis sp. growth at 100%. Presence of LPOS or Lactoperoxydase system with iodine (LPOSI) in chitosan at 0.5% increased the percentage of inhibition from 26 to 93%. Edible films with LPOS inhibited Phomopsis sp. RP257 particularly when LPOS was incorporated in chitosan concentrations of 1 and 1.5%. Iodine did not influenced antifungal activity of LPOS against Pestalotiopsis sp. but decreased activity antifungal toward Phomopsis sp. RP257. The properties (water vapor permeability and mechanical properties) of chitosan films were not significantly changed by the incorporation of the enzyme system. in vivo condition, chitosan coating at 1 and 1.5% with or without enzyme system was sufficient to inhibit totally (100%) Pestalotiopsis sp. and was 60% efficient against Phomopsis sp. with chitosan only at 1 and 1.5%. However, when coating solution mainly at 1 and 1.5% was enhanced by LPOS with or without iodine, it inhibited totally (100%) Phomopsis sp. RP257. The presence of iodine slightly reduced antifungal activity against Phomopsis sp. RP257. 相似文献
35.
Microstructure and protein digestibility of beef: The effect of cooking conditions as used in stews and curries 总被引:1,自引:0,他引:1
Beef meat was cooked at 373 K for 10 and 30 min to investigate the effect of the cooking conditions generally used during beef stew and curry preparation on protein digestibility. The cooked meats, along with a raw control, were digested using an in vitro digestion model to simulate gastric and small-intestinal conditions. Samples taken at different digestion times were analyzed using SDS-PAGE, RP-HPLC, ninhydrin assays for amino N and transmission electron microscopy. Simulated gastric conditions quickly led to the loss of basic sarcomere structure in raw meat myofibrils whereas the sarcomere structure of the compact cooked meat myofibrils remained intact after 30 min of gastric digestion. Prolonged cooking of meat (30 min) resulted in incomplete digestion of small MW (<10 kDa) peptides, as observed from SDS-PAGE. This agreed with the amount of ninhydrin-reactive amino N released during digestion, which decreased with an increase in cooking time. The RP-HPLC peak areas of the major identified amino acids (tryptophan, tyrosine and phenylalanine) also decreased with an increase in cooking time. This suggested the formation of “limit peptides” during prolonged cooking of beef, which were not further broken down into free amino acids by digestive enzymes and therefore might not be bioavailable. 相似文献
36.
Thermoneutrality results in prominent diet‐induced body weight differences in C57BL/6J mice,not paralleled by diet‐induced metabolic differences 下载免费PDF全文
37.
Coma V Portes E Gardrat C Richard-Forget F Castellan A 《Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment》2011,28(2):218-225
Many plant pathogens produce toxic metabolites when growing on food and feed. Some antioxidative components seem to prevent fungal growth and mycotoxin formation. Recently, we synthesized a new class of powerful antioxidative compounds, i.e. tetrahydrocurcuminoids, and its structure/antioxidant activity relationships have been established. The South West of France produces large amounts of corn, which can be infected by Fusarium species, particularly F. proliferatum. In this context, the efficiency of tetrahydrocurcuminoids, which can be obtained from natural curcuminoids, was investigated to control in vitro the growth of F. proliferatum and the production of its associated mycotoxin, fumonisin B?. The relation between structure and antifungal activity was studied. Tetrahydrocurcumin (THC1), with two guaiacyl phenolic subunits, showed the highest inhibitory activity (measured as radial growth on agar medium) against the F. proliferatum development (67% inhibition at a concentration of 13.6 μmol ml?1). The efficiencies of THC2 (36% at a concentration of 11.5 μmol ml?1), which contains syringyl phenolic units, and THC3 (30% at a concentration of 13.6 μmol ml?1), which does not have any substituent on the aromatic rings, were relatively close. These results indicate that the simultaneous presence of guaiacyl phenols and the enolic function of the β-diketone moiety play an important role in the inhibition mechanisms. The importance of this combination was confirmed using n-propylguaiacol and acetylacetone as molecular models. Under the same conditions, ferulic acid and eugenol, other natural phenolic antioxidants, were less efficient in inhibiting fungal growth. THC1 also reduced fumonisin B? production in liquid medium by approximately 35, 50 and 75% at concentrations of 0.8, 1.3, and 1.9 μmol ml?1, respectively. These very low inhibitory concentrations show that tetrahydrocurcuminoids could be one of the most promising biobased molecules for the control of mycotoxinogen fungal strains. 相似文献
38.
Measurements of anthropogenic radionuclides in marine sediments can provide good estimates of past and present radioactive contamination of the marine environment. The Barents Sea is one of the world's richest ocean areas, and it is therefore of major interest for Norwegian fisheries to document the levels of radioactive contamination of this and adjacent ocean areas. In this study, concentrations of 137Cs, 238Pu, (239,240)Pu and 241Am were measured in marine sediments collected mainly in the Spitsbergen-Bear Island region. Additional samples collected in the deep Norwegian Sea and near the Greenland ice-edge have been included for comparison. The highest radionuclide levels were found near Spitsbergen, reaching over 50% higher than in adjacent areas. Determinations of the mineral content and particle size distribution indicate a relation between the content of clay minerals and radionuclide levels. The present study confirms earlier observations of elevated levels of 137Cs in sediments in the Spitsbergen-Bear Island area and documents elevated levels of 238Pu, (239,240)Pu and 241Am in these sediments. Using an exponential curve fit based on determinations of unsupported 210Pb in sediment cores, sedimentation rates of 0.28, 0.05 and 0.26 cm year(-1) were calculated at locations south of Spitsbergen, near the Greenland ice-edge and in the deep Norwegian Sea, respectively. 相似文献
39.
Marie Bisconti Jean-Franois Simon Sarah Grassi Baptiste Leroy Baptiste Martinet Vanessa Arcolia Vladimir Isachenko Elise Hennebert 《International journal of molecular sciences》2021,22(23)
Male infertility is a common health problem that can be influenced by a host of lifestyle risk factors such as environment, nutrition, smoking, stress, and endocrine disruptors. These effects have been largely demonstrated on sperm parameters (e.g., motility, numeration, vitality, DNA integrity). In addition, several studies showed the deregulation of sperm proteins in relation to some of these factors. This review inventories the literature related to the identification of sperm proteins showing abundance variations in response to the four risk factors for male infertility that are the most investigated in this context: obesity, diabetes, tobacco smoking, and exposure to bisphenol-A (BPA). First, we provide an overview of the techniques used to identify deregulated proteins. Then, we summarise the main results obtained in the different studies and provide a compiled list of deregulated proteins in relation to each risk factor. Gene ontology analysis of these deregulated proteins shows that oxidative stress and immune and inflammatory responses are common mechanisms involved in sperm alterations encountered in relation to the risk factors. 相似文献
40.
Claudia Fecher-Trost Karin Wolske Christine Wesely Heidi Lhr Daniel S. Klawitter Petra Weissgerber Elise Gradhand Christine P. Burren Anna E. Mason Manuel Winter Ulrich Wissenbach 《International journal of molecular sciences》2021,22(23)
Recently, we reported a case of an infant with neonatal severe under-mineralizing skeletal dysplasia caused by mutations within both alleles of the TRPV6 gene. One mutation results in an in frame stop codon (R510stop) that leads to a truncated, nonfunctional TRPV6 channel, and the second in a point mutation (G660R) that, surprisingly, does not affect the Ca2+ permeability of TRPV6. We mimicked the subunit composition of the unaffected heterozygous parent and child by coexpressing the TRPV6 G660R and R510stop mutants and combinations with wild type TRPV6. We show that both the G660R and R510stop mutant subunits are expressed and result in decreased calcium uptake, which is the result of the reduced abundancy of functional TRPV6 channels within the plasma membrane. We compared the proteomic profiles of a healthy placenta with that of the diseased infant and detected, exclusively in the latter two proteases, HTRA1 and cathepsin G. Our results implicate that the combination of the two mutant TRPV6 subunits, which are expressed in the placenta of the diseased child, is responsible for the decreased calcium uptake, which could explain the skeletal dysplasia. In addition, placental calcium deficiency also appears to be associated with an increase in the expression of proteases. 相似文献