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111.
112.
We describe an original microscope for high-resolution optical coherence tomography applications. Our system is based on a Linnik interference microscope with high-numerical-aperture objectives. Lock-in detection of the interference signal is achieved in parallel on a CCD by use of a photoelastic birefringence modulator and full-field stroboscopic illumination with an infrared LED. Transverse cross-section (en-face, or XY) images can be obtained in real time with better than 1-microm axial (Z) resolution and 0.5-microm transverse (XY) resolution. A sensitivity of approximately 80 dB is reached at a 1-image/s acquisition rate, which allows tomography in scattering media such as biological tissues. 相似文献
113.
Aequorin is a photoprotein originating from jellyfish, whose luminescent activity is dependent on the concentration of calcium ions. Due to the high sensitivity and low background linked to luminescent assays, as well as to its absence of toxicity and its large linear dynamic range, aequorin has been used as an intracellular calcium indicator since its discovery in the early 1960s. The first applications of aequorin involved its microinjection in cells. The cloning of its gene in 1985 opened the way to the stable expression of aequorin in cell lines or even entire organisms. Here we present the validation of aequorin as a functional assay for the screening of G-protein-coupled receptors, ion channels, and tyrosine kinase receptors, as well as for their pharmacological characterization in agonist and antagonist detection assays. We optimized our cell suspension-based assay and determined that the most sensitive assay was performed at room temperature, with mitochondrially expressed aequorin and using coelenterazine derivative h for reconstitution of aequorin. The robustness of the assay and the current availability of luminometers with integrated injectors allow aequorin to fit perfectly with high throughput functional assays requirements. 相似文献
114.
115.
Many neurons of the central nervous system are broadly tuned to some sensory or motor variables. This property allows one to assign to each neuron a preferred attribute (PA). The width of tuning curves and the distribution of PAs in a population of neurons tuned to a given variable define the collective behavior of the population. In this article, we study the relationship of the nature of the tuning curves, the distribution of PAs, and computational properties of linear neuronal populations. We show that noise-resistant distributed linear algebraic processing and learning can be implemented by a population of cosine tuned neurons assuming a nonuniform but regular distribution of PAs. We extend these results analytically to the noncosine tuning and uniform distribution case and show with a numerical simulation that the results remain valid for a nonuniform regular distribution of PAs for broad noncosine tuning curves. These observations provide a theoretical basis for modeling general nonlinear sensorimotor transformations as sets of local linearized representations. 相似文献
116.
A Unifying and Rigorous Shape from Shading Method Adapted to Realistic Data and Applications 总被引:1,自引:0,他引:1
Emmanuel Prados Fabio Camilli Olivier Faugeras 《Journal of Mathematical Imaging and Vision》2006,25(3):307-328
We propose a new method for the Lambertian Shape From Shading (SFS) problem based on the notion of Crandall-Lions viscosity solution. This method has the advantage of requiring the knowledge of the solution (the surface to be reconstructed) only on some part of the boundary and/or of the singular set (the set of the points at maximal intensity). Moreover it unifies in an unique mathematical formulation the works of Rouy et al. [34, 50], Falcone et al. [21], Prados et al. [46, 48, 49], based on the notion of viscosity solutions and the work of Dupuis and Oliensis [17] dealing with classical solutions and value functions. Also, it allows to generalize their results to the “perspective SFS” problem recently simultaneously introduced in [13,46,55]. While the theoretical part has been developed in [44], in this paper we give some stability results and we describe numerical schemes for the SFS based on this method. We construct provably convergent and robust algorithms. Finally, we apply our SFS method to real images and we suggest some real-life applications. 相似文献
117.
Klesges Robert C.; DeBon Margaret; Vander Weg Mark W.; Haddock C. Keith; Lando Harry A.; Relyea George E.; Peterson Alan L.; Talcott G. Wayne 《Canadian Metallurgical Quarterly》2006,74(2):295
The authors evaluated the effect of a brief tailored smoking control intervention delivered during basic military training on tobacco use in a population of military personnel (N = 33,215). Participants were randomized to either a tobacco use intervention (smoking cessation, smokeless tobacco use cessation, or prevention depending on tobacco use history) or a health education control condition. Results indicated that smokers who received intervention were 1.16 (95% confidence interval [CI] = 1.04, 1.30) times (7-day point prevalence) and 1.23 (95% CI = 1.07, 1.41) times (continuous abstinence) more likely to be abstinent than controls from smoking cigarettes at the 1-year follow-up (p p 相似文献
118.
Bradley T. Vander Zanden Richard Halterman Brad A. Myers Rob Miller Pedro Szekely Dario A. Giuse David Kosbie Rich McDaniel 《Software》2005,35(13):1275-1298
One‐way constraints have been incorporated in many graphical user interface toolkits because they are simple to learn, easy to write, and can express many types of useful graphical relationships. This paper is an evaluative paper that examines users' experience with one‐way constraints in two user interface development toolkits, Garnet and Amulet, over a 15‐year time span. The lessons gained from this examination can help guide the design of future constraint systems. The most important lessons are that (1) constraints should be allowed to contain arbitrary code that is written in the underlying toolkit language and does not require any annotations, such as parameter declarations, (2) constraints are difficult to debug and better debugging tools are needed, and (3) programmers will readily use one‐way constraints to specify the graphical layout of an application, but must be carefully and time‐consumingly trained to use them for other purposes. Copyright © 2005 John Wiley & Sons, Ltd. 相似文献
119.
Roland Bouffanais Michel O. Deville Paul F. Fischer Emmanuel Leriche Daniel Weill 《Journal of scientific computing》2006,27(1-3):151-162
This paper presents the large-eddy simulation of the lid-driven cubic cavity flow by the spectral element method (SEM) using the dynamic model. Two spectral filtering techniques suitable for these simulations have been implemented. Numerical results for Reynolds number Re=12,000 are showing very good agreement with other experimental and DNS results found in the literature. 相似文献
120.
Controlling an approximation model of a controllable infinite dimensional linear control system does not necessarily yield a good approximation of the control needed for the continuous model. In the present paper, under the main assumptions that the discretized semigroup is uniformly analytic, and that the control operator is mildly unbounded, we prove that the semidiscrete approximation models are uniformly controllable. Moreover, we provide a computationally efficient way to compute the approximation controls. An example of application is implemented for the one- and two-dimensional heat equation with Neumann boundary control. 相似文献