Estimation of creep crack growth rate in IN-100 based on the Q* parameter concept

Since the high-strength Ni-based superalloy, cast IN-100, is considered to be brittle at high temperatures, the stable creep crack growth region is limited. Therefore, technically, it is very difficult to perform creep tests and there are few experimental results on the creep crack growth behaviour of this material. We performed creep crack growth tests using Ni-based superalloy, cast IN-100, and derived the Q* parameter for this material, which characterizes the creep crack growth rate. Using this Q* parameter, we derived a law for the creep rupture life of this material.     

Measurements of 220 GHz atmospheric opacity on Mt. Fuji with a radiometer/radome system

We measured atmospheric opacity at 220 GHz at the summit of Mt. Fuji (alt. 3776 m) about one year in order to explore a feasibility of submillimeter-wave astronomical observations. For this purpose, a 220 GHz radiometer system enclosed in a radome (51×51×62 cm³) has been developed. The 220 GHz opacity was lower than 0.06 for a significant fraction (～ 45 %) of time from November 1994 to March 1995. Diurnal variation of the opacity at the summit of Mt. Fuji is so small that continuous observation at submillimeter-wave is possible through day and night. Yearly variation of the opacity is studied from water vapor pressure data measured at the weather station for the past 3 years. To prevent accumulation of ice and snow on the Gore-Tex membrane in the radome, the outer membrane was supplied with a thermal flux of 0.63 kW m^?2 and the adjacent metal radome surfaces were supplied with a flux of ～0.9 kW m^?2. We evaluate from the 220 GHz transmission data that this heat flux is sufficient to keep the membrane on the radome free of ice and snow during 83 % of the time in 5 winter months. The summit of Mt. Fuji appears to be a promising site for submillimeter-wave observations.     

Factors affecting the optical properties of Pd-free Au–Pt-based dental alloys

The optical properties of experimental Au-Pt-based alloys containing a small amount of In, Sn, and Zn were investigated by spectrophotometric colorimetry to extract factors affecting color of Au-Pt-based high-karat dental alloys. It was found that the optical properties of Au-Pt-based alloys are strongly affected by the number of valence electrons per atom in an alloy, namely, the electron:atom ratio, e/a. That is, by increasing the e/a-value, activities of reflection in the long-wavelength range and absorption in the short-wavelength range in the visible spectrum apparently increased. As a result, the maximum slope of the spectral reflectance curve at the absorption edge, which is located near 515 nm (approximately 2.4 eV), apparently increased with e/a-value. Due to this effect, the b*-coordinate (yellow-blue) in the CIELAB color space considerably increased and the a*-coordinate (red-green) slightly increased with e/a-value. The addition of a third element with a higher number of valence electrons to the binary Au-Pt alloy is, therefore, effective in giving a gold tinge to the parent Au-Pt alloy. This information may be useful in controlling the color of Au-Pt-based dental alloys.     

Optical properties and microstructures of Pd-free Ag–Au–Pt–Cu dental alloys

Nine experimental Pd-free Ag–Au–Pt–Cu dental alloys containing 10 at.% Pt and 10–35 at.% Au were prepared and their optical properties and microstructures were investigated by means of spectrophotometric colorimetry, optical microscopy, and electron probe microanalysis. All the alloys were annealed at 850 °C and mirror-polished to observe their reflectance curves in the visible spectrum and three-dimensional color coordinates. All the alloys were composed of a major phase of Ag–Au-rich matrix and a minor and almost colorless Pt–Cu-rich phase. It was found that the color of the alloys was substantially controlled by the Ag–Au-rich matrix and that with increasing Au/Ag atomic ratio from 0.130 to 0.996, the yellow-blue chromaticity index b ^* increased from 8.0 to 14.4, giving a pale yellow color. This systematic increase in yellowness was caused by a continuous shift of the absorption edge of reflectance curve toward longer wavelengths with increasing Au/Ag atomic ratio.     

Ethanol-tolerant Saccharomyces cerevisiae strains isolated under selective conditions by over-expression of a proofreading-deficient DNA polymerase δ

Ethanol damages the cell membrane and functional proteins, gradually reducing cell viability, and leading to cell death during fermentation which impairs effective bioethanol production by budding yeast Saccharomyces cerevisiae. To obtain more suitable strains for bioethanol production and to gain a better understanding of ethanol tolerance, ethanol-tolerant mutants were isolated using the novel mutagenesis technique based on the disparity theory of evolution. According to this theory evolution can be accelerated by affecting the lagging-strand synthesis in which DNA polymerase δ is involved. Expression of the pol3-01 gene, a proofreading-deficient of DNA polymerase δ, in S. cerevisiae W303-1A grown under conditions of increasing ethanol concentration resulted in three ethanol-tolerant mutants (YFY1, YFY2 and YFY3), which could grow in medium containing 13% ethanol. Ethanol productivity also increased in YFY strains compared to the wild-type strain in medium containing 25% glucose. Cell morphology of YFY strain cells was normal even in the presence of 8% ethanol, whereas W303-1A cells were expanded by a big vacuole. Furthermore, two of these mutants were also resistant to high-temperature, Calcofluor white and NaCl. Expression levels of TPS1 and TSL1, which are responsible for trehalose biosynthesis, were higher in YFY strains relative to W303-1A, resulting in high levels of intracellular trehalose in YFY strains. This contributed to the multiple-stress tolerance that makes YFY strains suitable for the production of bioethanol.